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We narrowed to 993 results for: Des

Showing: 381 - 400 of 993 results

  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ... and the Puromycin resistance gene as separate peptides. PX459 This is the vector backbone. This particular...Protocols Paper for more information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from... the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann, et al. ...Guide Pages Browse Our CRISPR Collection Find gRNA Design Tools ...

  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...select the optimal dye or fluorescent ligand when designing an experiment. You may find yourself defaulting...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include..., which is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's...fluorescent donors and acceptors in their product descriptions, or technical manuals. Specific applications...signal-to-noise ratios. Certain dyes have been specifically designed for use in super-resolution microscopy techniques...

  3. Validated gRNA Sequences

    Type
    Collection
    ...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA...the particular conditions of the experiment as described in the associated article (listed below by PubMed...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...within promoters. When possible, the categories described on Addgene's CRISPR Plasmids and Resources page... to indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable...

  4. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ... producing a wall of results that can only be described as bigly. But such a project should not be undertaken...Library Screening Why the kinome? While properly designed perturbations – in the case of screens using CRISPR...References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects...with CRISPR/Cas9 Get John Doench's Tips on gRNA Design Check Out News Ways to Use CRISPR for Single Base...

  5. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...lab earlier, to read that stack of papers on your desk, and to tackle some new experiments before the next...gathering that pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project...labels. Color code your Post-its and write brief descriptions on each. Place the notes sticking up out of ...colleagues. Start small by identifying the protocols, guides, or references that your labmates ask to borrow...

  6. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...and industry, often means moving from the lab to a desk, although there’s often technical career tracks ...

  7. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some systems including Cas9), Cas...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-... sequences in the CRISPR array that will become guides for future targeted degradation. Before integration...biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources...

  8. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally... 2022). So the next time you see an antibody description with the Fc domain as only providing structure...

  9. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

  10. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ...vast collection of empty backbones that have been designed, tested, and published by academic scientists....find the vector that fits your experiments, I've described below some of the most frequently requested vectors...protein with a specific resin (e.g. 6xHis containing pDEST-HisMBP). After purification the protein can be released...vectors. Consider your applications and experimental design. You can create a new strain with a disrupted gene...

  11. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...errors in the design of cloning strategies set experiments back days or even weeks. Primer design was done ... alleviate these problems through good software design. But what makes software good? Fortunately, that...we dug into the original literature, some of it decades old, to provide reliable feature annotations. Even...

  12. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ... What are epitope tags? Epitope tags are short peptides introduced at the N or C terminus of a protein...purification or detection of the tagged protein. These peptides consist of as little as 8 or as many as several...need monoclonal GFP antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential...Protein folding issues, steric hindrances, and destabilization can be brought about by the tag so it’s important...

  13. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...resources to provide them to their students. My desire to create a set of low-cost, easy-to-use educational...protein – you first need the DNA sequence that encodes it. That DNA sequence then needs to be incorporated...one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable to many schools...students. In our BioBits kit for example, we’ve designed FD-CF experiments that result in outputs that ...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ... experimental design. How-to guide (Link opens in a new window) for the molecular design and testing of...interesting in implementing intersectional experimental design. The Deisseroth Lab maintains a Standard Operating...Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1):45-59. PubMed (Link...

  15. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...purification is now a reality thanks to OptoNBs. Recently designed by the Toettcher Lab, OptoNBs are photoswitchable... put AsLOV2 because here it was less likely to destroy the nanobody’s function than if inserted into the...blue-light induced binding. The lab used this general design scheme to generate two more OptoNBs, one against...exciting because using light to control signaling provides greater spatiotemporal resolution than pharmacological...

  16. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...but can be minimized with high powered research designs. Unanticipated discoveries Finally, there are good...rates, should be reported as a new discovery that deserves confirmation with new data and a new, preregistered...Guidelines; preregistration to increase clarity in study design and analysis; removing publication bias with Registered...Science, Dr. Mellor worked with citizen scientists to design and implement authentic scientific research and...

  17. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...Harvard University. In my previous two posts, I’ve described the fundamentals of how to work with Drosophila...Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists to study....e. there won’t be positioning effects). When designing experiments where you want to express multiple...wondering, “How do I know which of my flies have the desired integration?” Not to worry! In flies, phenotypic...

  19. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...’s social media toolbox. Besides advice for using Twitter, this post provides suggestions for using LinkedIn...use Twitter as a scientist. While this post is designed with beginners in mind, there’s some great reminders...sheet from Science Media Savvy This tip sheet provides advice for tweeting and blogging as a scientist...

  20. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...target specific mRNA transcripts for degradation. Despite how easy these experiments are to physically set...to prevent the incorporation of non-specific nucleotides into the dsRNA leading to increased RNAi efficiency... Lab’s recent Nature Biotechnology paper, they describe TurboID and miniTurbo, which utilize non-toxic...switch to be expressed. In this case, the gene encodes the LacZ𝛼 protein. Once expressed, LacZ𝛼 complexes...
Showing: 381 - 400 of 993 results