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...References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw, C. N., Banks, K. G., Ho Sui, S. J. Fulton,...Depositor Collections Pleiades Promoter Plasmids Pleiades Promoter Project The Pleiades Promoter Project (... These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters MiniPromoter...Plasmids from the Pleiades Promoter Project - A regulatory toolbox of MiniPromoters to drive selective... through these human MiniPromoters. Browse the Pleiades Promoter Plasmids using the table below. Additional...

...viral vector guides and plasmids available at Addgene and other related resources.... Science Guides Viral Vectors...Vectors Viral Vector Guides and Plasmids Selecting a Viral Vector Type Introduction to Viral Vectors Resources...selection of in-stock viral preps available. Browse Guides and Plasmids Lentiviral Vector Guide | Lentiviral...the production of viral vectors that contain the desired genetic cargo. In turn, these viral vectors are...

...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of Cas5... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink,...

...Pietro De Camilli 194005 VPS13D1-1576-(Y1253S) VPS13D GFP CMV Spinocerebellar Ataxia Pietro De Camilli...Parkinsonism Pietro De Camilli 232865 mCherry-VAB(VPS13C) VPS13C mCherry CMV Parkinsonism Pietro De Camilli 232866...Parkinsonism Pietro De Camilli 232867 VPS13C^Halo-delta(ATG2C-PH) VPS13C Halo CMV Parkinsonism Pietro De Camilli ...Parkinsonism Pietro De Camilli 232869 mCherry-PH(VPS13C) VPS13C mCherry CMV Parkinsonism Pietro De Camilli 232872...Parkinsonism, early-onset Pietro De Camilli 224252 pDest17_hnRNPA1_allW HNRNPA1 His T7 ALS Tanja Mittag...145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin 1-170 SYNJ1...SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin 1-145 SYNJ1 GFP...

...monocot and dicot plants. Plant gene editing through de novo induction of meristems . A comprehensive multi-purpose...and collections are designed for creating plant expression vectors. ID Kit Description Cloning Type PI 1000000036...collections contain plasmids designed for genome editing in plants. ID Kit Description Gene Editing Type PI 1000000076...It also includes plasmids encoding silencing suppressors, such as p19. ID Plasmid Description PI Additional...contains many plasmid tools that were specifically designed for use in plants. These resources include tools...expression to reporter systems. Addgene’s collection includes plasmids containing promoters that drive general...promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Synthetic Biology ...

...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...Han, Y., Ogrodowski, P., Crippa, A., Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014)...., Boix, C., Perrouault, L., Tesson, L., Geny, S., De Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli...application (see: gRNA Design Software ). In addition to an optimized gRNA design, engineered Cas9s can... the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit...

...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...BrainSpan Atlas of the Developing Human Brain - includes developmental transcriptome information, prenatal...

...LuxSit-i and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...Structurally destabilized firefly luciferases : A set of three increasingly destabilized mutants which...mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion of...plasmids. ID Plasmid Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc...your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top Other Highlighted...

...X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014. Genome-Scale CRISPR-Cas9...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows... store a record of invading phages but also to destroy the phages upon re-exposure. More specifically,...complex binds to the foreign DNA and cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview...in vitro. In 2013, Cong et al. and Mali et al. described CRISPR-based genome editing in mammalian cell ... of new applications. The first CRISPR papers described two main categories of genome edits. First, the...very low off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR...

..., Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science . 2014 Jan 3;343(6166...oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed based on...also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids...digested using BbsI, and a pair of annealed oligos (design is indicated below) can be cloned scarlessly into...vector before the sgRNA scaffold. The oligos are designed based on the target site sequence (20bp) and need...using SAM: Addgene plasmids #61422 - #61424 were designed for transient transfection : 61422 : Expresses... references are below. Available plasmids are described below: 61591 : PX601; CMV-driven SaCas9; U6-driven...

... cell reprogramming factors and wait for cells to de-differentiate. However it may be difficult to decide...blog post Delivery Methods for Generating iPSCs provides an overview of several different reprogramming...find more information. Delivery Method Species Description Article PI Lentivirus Human Expression of human...

...used for gRNA design. Read more about how to design your gRNA . Synthesize and Clone Desired gRNAs Once ...multi-plasmid SAM system Activate Design and Clone Your gRNA Achieving your desired edit comes down to location...on proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...selected, it’s time to design your gRNA oligos and clone them into your desired vector. In many cases,...experiment, including selecting the edit type, designing and cloning a gRNA, delivering Cas and the gRNA... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment...

...CRISPR/Cas design (Link opens in a new window) Program for designing optimal gRNAs. Provides feedback on...window) This tool helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...window) CRISPR/Cas9 reagent generation; gRNA design; HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor...CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums. CRISPR...protoSpaceJAM is an all-around platform for CRISPR knock-in design at scale. It uses an open-source algorithm to automate... automate and optimize gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and...

...Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal...pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden Gate ...Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden Gate TALEN...TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination Golden Gate TALEN 2.0...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome ...with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI FusX TALEN Assembly System Contains... pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru and Chad...

...CMV GFP DEST - Lentiviral Gateway destination vector for gene expression pLenti CMV/TO Zeo DEST - Tet-inducible...lentiviral vector for ORF expression pDest-565 - Gateway destination vector for bacterial expression; His...Membrane localization pWZL-Neo-Myr-Flag-DEST - pDest vector that adds an N-terminal myristoylation...marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral Gateway destination vector, expression, CMV/...pLenti CMV/TO Zeo DEST - Tet-inducible lentiviral Gateway destination vector ...expression pLenti CMV GFP DEST - Lentiviral Gateway destination vector for gene ...needed for replication in the host cell and are designed for a user to insert a gene of interest. They ...

... pii: 56469. Edwin Chapman Neuropeptides GRAB biosensors for neuropeptides VIP, NPY, CCK, NTS, UCN, SST... orexin neuropeptides A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nat Methods...cerevisiae cpFRET Kit includes plasmids to construct custom biosensors ScEnSor Kit includes fluorescent reporters... Resources Fluorescent biosensors are proteins designed so that their fluorescent properties are sensitive... or organisms for long-term imaging and can be designed to specifically localize to cellular compartments...compartments or organelles. Addgene’s database includes many fluorescent biosensors that signal the presence ...environmental conditions. With proper experimental design, biosensors can enable investigation of a signaling...

...the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the genome of...characterized which allow researchers to express desired constructs in precise cell types and/or developmental...that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins...expression plasmids. Large collections or kits are described below and not included in this table. For a more...search Addgene's entire collection. ID Plasmid Description Gene/Insert PI Plasmids Expressing Worm Genes...strains. CRISPR plasmids from John Calarco's lab as described in Heritable genome editing in C. elegans via ...genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient Genome Editing...

...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in C. elegans... CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian...Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast...Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use in Xenopus . ...Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links to.... Zebrafish CRISPR plasmids designed for use in zebrafish....