We narrowed to 672 results for: FIE

1. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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   Blog Post

   ...Meeting seven other times I had subsequently changed fields and my cohort of friends and colleagues had moved...effort in the future to match people within similar fields (ASM Microbe has eight tracks), something we didn...

2. Transferable Skills Guide: Career Planning Resources

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   Blog Post

   ...and other online career resources Once you’ve identified places you’d like to work, get online and take... a personal connection. That way, once you’ve identified an appropriate job opening, you can send your...

3. Plasmids 101: Gateway Cloning

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   Blog Post

   ...and attR sites.   Gateway vectors contain modified versions of the att sites so that scientists can...thanks to the Gateway vectors’ design. They have modified versions of the attB, P, L and R sites that recombine...

4. CRISPR-based Genome Editing Systems For Editing in E. coli

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   Blog Post

   ...into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated it into a...Sun B, Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted ...

5. Save Time with Transient Plant Leaf Transformations

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   Blog Post

   ...method with Agrobacterium: If you are new to the field, the following are useful tips for your first transient... Li, Jian-Feng, et al. "The FAST technique: a simplified Agrobacterium-based transformation method for...

6. New Tools Enable CRISPRa for Neuroscience Applications

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   Blog Post

   ... must be screened to identify effective ones. Simplified in vivo delivery using SPH mice CRISPRa has largely...mouse (Zhou et al., 2018). This mouse expresses a modified version of the “Suntag” Cas9 activator that recruits...

7. Data Freedom: The Expansion of Data Sharing in Research Publications

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   Blog Post

   ... was necessary and reasonable. PLOS has since clarified their data sharing policy and recently announced... PLOS’s credit, the organization subsequently clarified what the policy intent was and provided an FAQ...

8. Hot Plasmids: Summer 2024

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   Blog Post

   ...with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), magnetic bead...methyltransferase DNMT3A to the genomic location specified by the DNA-binding protein. The ADD domain of ...

9. Antibodies 101: Introduction to Gating in Flow Cytometry

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   Blog Post

   ...virtually all kinds of experiments and research fields, for any experiment, your first three gates will.../SSC gate.   Single-cell gating Once you have identified and gated on your cells of interest, you will...

10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    Blog Post

    ...from general note taking tools, to more scientific field-based solutions or open source solutions. In comparison...of budget and ease of use of the software. We identified that when talking about ELN costs and limited...

11. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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    Blog Post

    ... over millimeter-long distances across multiple fields of view, whereas previous Brainbow technologies...useful for discriminating neurons within only one field of view. Tetbow can be introduced in vivo either...

12. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post

    ...vitro by measuring the fluorescence intensity of purified protein at different acid concentrations. Overviews...Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

13. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

14. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...collaborators, others you know in the field, and even people who aren’t in the field. One PI on the panel even suggested...

15. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...materials, and resources that you’ve thoroughly identified. Do online searches, and seek advice from your...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

16. Lentivirus ddPCR Titration

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    Protocol

    ...response element (RRE) in the target cells but can be modified for other targets. Primers and a probe against... on their particular sample. This protocol was modified from the publication Wang et al. (2018) . Before...

17. CRISPR 101: Off-Target Effects

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    Blog Post

    ...mutating a specific amino acid or introducing a specified edit). However, if your design does have wiggle...depend on a DNA mutation, off-targets must be quantified slightly differently. For a comprehensive approach...

18. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post

    ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

19. Is this the right place for me? 8 tactics for choosing a lab

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    Blog Post

    ...leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management...

20. Technique: Probe Phage Genomes for Host Binding Proteins

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    Blog Post

    ...assay, including examples of RBPs successfully identified using this method. Step 1: Prepare a random fragment...Elisabeth A. Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical...