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Showing: 381 - 400 of 665 results

  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...and expression, the recombinant antibody can be purified for use in a variety of applications. Sharing ... used as-is or the recombinant antibody can be purified from the supernatant (see our Recombinant Antibody...

  3. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... and downstream (3’) of the DNA segment to be amplified. When these reagents are combined in an appropriate... After the PCR is complete, the product can be verified based on size by gel electrophoresis . Protocol...

  6. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...you can order plasmids covering a wide range of fields from Addgene. What you might not know is that Addgene...Illumina-M13F bridging primer. These bridging primers are amplified and used to generate Illumina sequencing libraries...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...with MagIC-cryo-EM. Nucleosome-like particles identified by Topaz software (green circles), magnetic bead...methyltransferase DNMT3A to the genomic location specified by the DNA-binding protein. The ADD domain of ...

  8. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...virtually all kinds of experiments and research fields, for any experiment, your first three gates will.../SSC gate.   Single-cell gating Once you have identified and gated on your cells of interest, you will...

  9. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...vitro by measuring the fluorescence intensity of purified protein at different acid concentrations. Overviews...Zacharias, David A., et al. "Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live...

  11. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...materials, and resources that you’ve thoroughly identified. Do online searches, and seek advice from your...eliminate from your budget. Every cut you make signifies that you've made a conscious effort to eliminate...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ...and stack as you pour. If your agar partially solidifies in the bottle while you’re pouring, you should...stored at 4 ℃ until use. Once your plates have solidified and dried, you should test them to make sure ...

  13. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...leave prematurely? Do lab alumni stay in the same field taking projects with them and remaining close colleagues...colleagues of the PI? Or do lab alumni leave the field or get pushed out by the PI? What is the management...

  14. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...assay, including examples of RBPs successfully identified using this method. Step 1: Prepare a random fragment...Elisabeth A. Raleigh. "Biosynthesis and Function of Modified Bases in Bacteria and Their Viruses." Chemical...

  15. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...mutating a specific amino acid or introducing a specified edit). However, if your design does have wiggle...depend on a DNA mutation, off-targets must be quantified slightly differently. For a comprehensive approach...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail of the box in C. Image adapted from Andrews...whichever targets you like. Plus, indirect IF amplifies the signal since multiple secondary antibodies...

  18. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... grad school, you searched for and eventually identified a postdoctoral mentor. A good mentor typically...yourself to and interact with the members of your field, and present your research. Face to face meetings...

  19. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...Lentivirus Production and Transduction Protocols   Once purified, you must titrate your viral particles to determine...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...multi-color imaging (Figure 2). If you’re working with purified proteins in vitro, you can even use the same tag...649-mDia1 and 549-CP are both SNAP-tag fusions, purified and labeled separately with different dyes. b)...
Showing: 381 - 400 of 665 results