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We narrowed to 856 results for: HA

Showing: 381 - 400 of 856 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...facilities, tissue culture labs and every cell culturist has to deal with this problem. It is estimated that mycoplasma...this induces a noticeable color change. This method has high sensitivity but low specificity (Degeling, 2012...affect the cell growth and viability. If mycoplasma has made permanent damage to the cell culture it is irreparable...Institute of Biosciences and Technology in India. Jadhav has a Master's degree in Molecular Biology (University...

  2. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...developed for use in mammalian cells, this system has also been implemented in a variety of other organisms...Drosophila cells. Work by Wei et al. from Kang Shen’s lab has also shown that the Q system is also functional in...elegans. Subsequent work by the Luo and Potter labs has refined the Q system and introduced the second generation...multiple genes in synthetic systems. Recent work has even used all 3 in a single modular vector for ease...

  3. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing...that regulate a desired phenotype. In fact, CRISPR has already been used to engineer the genome of many ...to protoplast cells (plant cells whose cell wall has been removed). Another common delivery method is ...developed for plants and prime editing for plants has been developed for rice by Yiping Qi’s lab and for...

  6. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...Hack Day lunches These days everyone is busy and has a To-Do list long enough that there is no hope of...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams. ...

  7. Common Injection Routes in Mice

    Type
    Blog Post
    ... of practice to ensure the proper injection site has been located and to avoid potential off-target pokes...researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing...: intermediate Your decision Hopefully this post has provided a good introduction to common injection ...

  8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...the bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition...

  10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...DRSC/TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down...tools. The Vienna Drosophila Resource Center also has collections of flies for in vivo gene knock down ...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

  11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    ...methods for CRISPR activation, research in the area has continued to develop. These newer methods have not... of rearranging chromatin structure. This method has shown higher activation levels than SAM in some loci...-HSF activation domains used in SAM. This method has been shown to activate genes at 2- to 3-fold higher...

  12. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the...always look at your antibody datasheet to see if it has been tested in IF and if a protocol is available....an effective strategy. (This article on fixation has some more examples to help choose which fixative ...

  13. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting ...Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible...that is smaller and easier to deliver, but so far has few inhibitors identified.     New from the viral...

  14. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...biosensors, and generally as biological tools, our blog has you covered. Happy voltage sensing!   Figure...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of the sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have...the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ’s dominance ...

  16. Guide to Using Pooled Libraries

    Type
    Guide
    ...Toronto KnockOut (TKO) library from the Moffat lab has over 175,000 different gRNA-containing plasmids)....

  17. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...expensive, it may be helpful to find an antibody that has already been validated for flow cytometry through...Characterization through Open Science) initiative has now started producing flow cytometry characterization...overexpression. Additionally, if the cell line used has endogenous levels of expression, this can make the...TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop...as part of the YCharOS open science ecosystem. It has received in-kind support to produce this data from...

  18. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...research and PhD studies, there lies a hidden gem that has the potential to redefine the course of one's research...inspiration. Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint...

  20. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...cooperative education experience (co-op) is what has brought me, a second year bioengineering student,... that remote work would feel isolating, but that has not been the case for me at Addgene. There is a clear...
Showing: 381 - 400 of 856 results