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We narrowed to 1,034 results for: LAS

Showing: 381 - 400 of 1034 results

  1. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post
    ...Addgene.org Find plasmids in Addgene's COVID-19 collection Find SARS-COV-2 pseudotype virus plasmids from Addgene...variants. “We created a series of spike expressing plasmids which encode spikes found on all of the different...response. The Balazs lab was quick to deposit their plasmids at Addgene to accelerate ongoing COVID-19 research...research. “I hope that by sharing the plasmids other scientists would be able to expand upon the work and...SARS-CoV-2 501Y.V2 from neutralization by convalescent plasma. Nature 593:142–146. https://doi.org/10.1038/s41586...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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    Blog Post
    ...In fact, an entire classification scheme, known as the Baltimore replication classes, has been developed...replicate (Figure 2). Figure 2: Baltimore replication classes. Image credit: Louten, 2016 Viruses with DNA genomes... an RNA or dsDNA intermediary, depending on the class of virus. RNA genomes can be transcribed into mRNA... of RNA or DNA intermediaries, depending on the class of virus. From one, come many After a virion infects...the required proteins are packaged in separate plasmids and expressed via cells cultured with the vectors...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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    Blog Post
    ...for short. Addgene has already distributed this plasmid all over the world, and the question on everyone...identified NgAgo and showed that it can cleave plasmid and genomic DNA in a variety of mammalian cell ...homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and... contrast, RNA guides must be expressed from a plasmid or in vitro transcribed, and changes in gRNA secondary.... Epub 2016 May 2. PubMed PMID: 27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan...24531762. PubMed Central PMCID: PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, ...

  4. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    Blog Post
    ...studying four of those rare diseases will receive plasmids from Addgene worth a total of $5000.  Lowe Syndrome...involving the primary cilia. They’ll put a variety of plasmids to work to explore the effect of various OCRL1...there. This will help us acquire a lot of those plasmids so that we can try many different things in parallel..., will use GFP-ATP1A3, FLAG-ATP1A3, and ATP1B3 plasmids to explore the functional differences among mutations...experienced by AHC patients. Waardenburg Syndrome Last but not least, Meng-Shin Shiao (right) of Mahidol...mutations. More specifically, they’ve proposed to use plasmids with modified nickase Cas9 protein in order to...

  5. RNA Interference in Plant Biology: New Tools for an Old Favorite

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    Blog Post
    ...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr... patens Reveals a Dominant Role of Augmin in Phragmoplast Microtubule Generation. Plant Cell 24:1478–1493...Steinmetz LM, Drewes G, Huber W (2019) Biological plasticity rescues target activity in CRISPR knock outs....

  6. With Our New Viral Service, We're Taking CRISPR Further!

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    Blog Post
    ...receive transfer plasmid DNA that you can use along with lentiviral packaging plasmids to create your own...rough approximations of the total number of CRISPR plasmids for that particular organism available at Addgene...helped distribute many of these technologies in plasmid form and are proud to have facilitated their fast...fast adoption. However, in many cases the plasmids themselves are only the starting point for the production... use; after receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, ...

  7. Cultivating Community Science at BosLab

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    Blog Post
    ...Kotton Learn How Plasmid Sharing Speeds Science Resources at Addgene.org Order Plasmids from Addgene! ...individual and community science projects, we offer classes, workshops, book clubs, seminars, and social events...popular. People kept asking if we were teaching classes at the lab, so in December 2015 we held our first...started. For example, Timothy Stiles joined BosLab last year with a computer science degree and had zero...to engineer microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast...

  8. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited...deposited at Addgene was used to produce the glioblastoma model for this study (Ref 6)). In addition, it... immunology resources. If you have a great new plasmid you’d like to share with the scientific community...

  9. GCE4All: Making Genetic Code Expansion Accessible

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    Blog Post
    ...often incorporated into the cells via plasmid. Third, a second plasmid will be needed to introduce the gene...and synthetase introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new...the cell’s genome, instead of incorporation via plasmid or media. Theoretically, if you put all these together...Blue Flame award to identify highly requested GCE plasmids. But they’d also love scientist interested in,...and resources, and then checking out all of the plasmids and bacterial strains (available at Addgene for...

  10. Which Fluorescence Microscopy Techniques is Best for Me?

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    Blog Post
    ...biological tissue. To prove this, simply hold a flash light to the palm of your hand. Although white light... that when it reaches the interface between the glass coverslip and a sample in aqueous buffer it is completely...due to the refractive index mismatch between the glass and the water-like buffer the sample is immersed...fluorophores within a few hundred nanometers of the glass/water interface. Super resolution microscopy Super...sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus of the experiment...very gentle on the sample as the majority of the laser light is reflected away from the sample and excitation... Find Empty Fluorescent Protein Backbones Find Plasmids for Optogenetics ...

  11. Celebrating 15 Years of Scientific Sharing

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    Blog Post
    ...recognize! Distributing plasmids, viral service, and more For the first time in the last couple of years, ...to worry about succeeding at our core mission. Plasmid distribution is amazingly still growing at >9% ...distribution to ensure scientists everywhere can access plasmids despite the evolving export/import rules. Our ...Speaking of the website, we now have a UI/UX team. Last time I checked we were ranked as the 21,806th most...Addgene Resources on the Addgene website Find plasmids for your research  Learn about our viral service...

  12. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...Split-BioID plasmids Find CRISPR plasmids for labeling genomic regions Find CRISPR plasmids for purifying...literature and analyzing data from The Cancer Genome Atlas, Ramanathan et al. found evidence that suggests ...question that RaPID could help answer? The RaPID plasmids are available from Addgene! References Ramanathan...PubMed Central PMCID: PMC5886736. Find the RaPID Plasmids Here! McHugh, C.A., Russell, P., & Guttman, M...

  13. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post
    ... with the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...highlight their research and how they will use plasmids and viral preps from Addgene to further their ...BeHeard award, the ACD will use Addgene’s RESOLUTE plasmid collection to perform a functional screen in mammalian...therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection consists of human and murine solute ...contraction defects. The lab also plans to use CRISPR plasmids to generate deletions of candidate genes in euploid...research questions,” Hébert says. In the spirit of plasmid sharing and open science, Hébert plans on building...

  14. Developing Lab Management Software for Biology

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    Blog Post
    ...quality, growth, and legal status of thousands of plasmids a day, like we do at Addgene, pen and paper will..., of course, within the lab. In order to get a plasmid to your lab bench, we need to (this is a little...  someone has put in a request for a particular plasmid and now it’s time for our lab technicians to retrieve...tray or a freezer. Some are disposable, like a plastic tube. Talk with your lab team about how they would...to be situations where you learn biology terms (plasmid, 5-prime, CRISPR, growth strains, vector backbone...Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

  15. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

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    Blog Post
    ...evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR is a ground-breaking...compared to eSpCas9(1.1) and SpCas9-HF1. The HypaCas9 plasmids are available from Addgene here! Future possibilities... (2015): aad5227. PubMed PMID: 26628643. Find plasmids from this publication at Addgene. 2. Nishimasu..." Nature (2016).  PubMed PMID: 26735016. Find plasmids from this publication at Addgene. 5. Chen, JS...." Nature (2017). PubMed PMID: 28931002. Find plasmids from this publication at Addgene. Resources on... Resources on the Addgene Website Find CRISPR Plasmids Browse our CRISPR Guide Pages Find Genome Engineering...

  16. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ...antibodies contain a mix of different isotypes and subclasses. They each have their own advantages. Since monoclonal...species, like an anti-mouse IgG antibody. Some are subclass specific, like an anti-mouse IgG2a antibody. When...    Figure 1: Broadly reactive versus subclass-specific antibodies. Only a fraction of the antibody... antibody (A). To boost signal, consider using subclass specific secondary antibodies instead (B). ...antibody contains a mix of different isotypes and subclasses, use a broadly reactive secondary antibody to...antibody you may wonder, “Will the isotype, and subclass affect my experiment?” Well, it depends on the...

  17. Tips for Technical Support Calls

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    Blog Post
    ..., make sure to mention the plasmid catalog number, or at least the plasmid name in your initial query....from researchers, and the vast majority of our plasmids and pooled libraries were originally described...links to later papers that successfully used said plasmids. We provide these references as our way of saying...to keep in mind after you hit “send” or dial that last digit: An answer should come soon, but not instantly...Asked Questions Pages Learn What to Do with Your Plasmids Once You Recieve Them Get Help with the Addgene...

  18. Adapting Toehold Switches to Detect Zika Virus

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    Blog Post
    ... Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...field of synthetic biology. The first was a new class of programmable riboregulators, called toehold switches...detect the virus directly from infected monkey blood plasma without purification or concentration of the virus...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander...Experiment Deposit Your Plasmids with Addgene ...

  19. Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!

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    Blog Post
    ...100,000 different plasmids available from the repository (currently at 98,276 plasmids as of April 8, 2021...re now sharing over 100,000 reagents including plasmids, ready-to-use viral preps, pooled libraries, kits... 2021) 1.5 million plasmids shared in total (currently at 1,484,507 as of April 8, 2021) 5,000 depositing...addgene.org homepage! You can track the number of plasmids right below the search bar and more stats further...

  20. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post
    ...imperative to the health of an organism that both classes of genes function normally to maintain balance ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...
Showing: 381 - 400 of 1034 results