We narrowed to 526 results for: MEA;

1. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   ...with an anti-HA antibody and serves as a backup means of detection that’s independent of the reporter....

2. Visualizing Protein Turnover In Situ

   Type

   Blog Post

   ...I was studying a synaptic protein, I wanted to measure it’s levels in its normal neuronal context. In ...

3. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   ...Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single...

4. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   ...image represents the composite of fluorescent measurements from red, blue, and green channels overlaid ...

5. Plasmid Tools for Microbiome Studies

   Type

   Blog Post

   ...generations can be determined. The lab used DCDC to measure growth rate of E. coli for over 10 generations ...

6. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   ...the cell membrane where cell voltage can then be measured. Archon1 is  bright, exhibits good localization...

7. Reproducibility for Everyone: Lessons from an Open Science Collaboration

   Type

   Blog Post

   ...reproducibility workshops and organizational skills. Meanwhile, Addgene was also thinking about ways to address...

8. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   ...cell-adhesion proteins) targeting different organs. This means that exosomes not only promote microenvironment’...

9. Antibodies 101: Conjugation

   Type

   Blog Post

   ...2017) Simultaneous epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https...

10. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    ...are initiated before the cell has divided. This means that genes located near the origin will be over-...

11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    ... known as the resting potential (Vmem), can be measured as the imbalance of electrical charge between ...

12. Developing Lab Management Software for Biology

    Type

    Blog Post

    ...Apache, and MariaDB. Using tried-and-true tools means less time getting up and running and more time working...

13. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type

    Blog Post

    ... and arbitrary decision. Unfortunately, it also meant that, by the very nature of the way the experiments...

14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    ...biological systems. The CombiGEM platform provides a means to create barcoded gRNA libraries that can be used...

15. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    ...opposed to purified DNA or colony sequencing. This means that we are sequencing from the exact same stock...

16. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ...specifc, easily read sequences that can be used to measure their clonal abundance before and after a treatment...

17. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type

    Blog Post

    ...very large number of regulators. That basically means larger genetic circuits that could conceivably be...

18. Career Coaching for Scientists: Why and Where Do I Find One?

    Type

    Blog Post

    ...transition out of the academic sphere into a different meaningful career in science. Supervisors in the academic...

19. Protocol - Bacterial Transformation

    Type

    Protocol

    ...bacteria but also because bacteria are used as the means for both storing and replicating plasmids. Because...applied to the cell/DNA mixture to induce membrane permeability. To do this you will need to have access to ...

20. Protocol - How to Ligate Plasmid DNA

    Type

    Protocol

    ...each other. When the sticky ends are compatible, meaning that the overhanging base pairs on the vector and...average size genes and vectors, this ratio is really meant to refer to the molarity of DNA ends available for...