We narrowed to 403 results for: NAM
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TypeBlog Post...Sleeping Beauty plasmids piggyBac Although its name suggests otherwise, piggyBac was discovered in the...
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An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...genetics.118.301775 Ghanta KS, Doksin GA, Mir A, Krishnamurthy PM, Gneid H, Edraki A, Watts JK, Sontheimer ... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...produced from long dsRNA via an RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...fluorophores carry their emission peaks within their names, for example, BV421 and RB545. Fluorophores can ... -
Antibodies 101: Flow Cytometry
TypeBlog Post...to line up in single file in a process termed hydrodynamic focusing. This allows each cell to pass through... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post..., USA 39.7392°N 104.9903°W - Erin Golden Hi, my name is Erin and I have spent the last 15 years training... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog Post...DNA scissors: the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post... ideal number. First you generate four unique kanamycin-resistant plasmids, each containing a different... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post... this approach to both the PE2 and PE3 systems, naming the new systems PE4 and PE5, respectively. These... -
Antibody Validation for Flow Cytometry
TypeBlog Post...the target. Helpfully, this also includes clone names, which can allow you to search different providers... -
Delivery Methods for Generating iPSCs
TypeBlog Post... References 1. Sano, Masayuki, Minoru Iijima, Manami Ohtaka, and Mahito Nakanishi. "Novel Strategy to... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...localization and expression. This base editor was named ABEmax. In 2020, two papers were published describing... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post...carries a spacer targeting the gene of interest and kanamycin resistance E. coli carrying the phage recombineering... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...Sullivan, T. P., Razmpour, R., Gong, Y., Ng, C., Nammour, J., Maiz, D., Dujardin, S., Ramirez, S. H., Hudry... -
CRISPR Guide
TypeGuide...as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 (CRISPR Type II) systems...Bxb1 installs the donor sequence. Bxb1 versions named evoBxb1 or eeBxb1 are evolved versions of the recombinase...CRISPR imaging can visualize chromatin and RNA dynamics in live cells. Labeling can be done by fusing ... Fluorescent CRISPR systems have been used for dynamic tracking of repetitive and non-repetitive genomic...Cse1), Cas11 (Cse2), Cas7, Cas5 and Cas6e. As the name implies, this system is harnessed for antiviral ...Weissman, J. S., Qi, L. S., & Huang, B. (2013). Dynamic imaging of genomic loci in living human cells by... -
Sequencing Primers
TypeGuide...Biology Reference page . Universal Sequencing Primers Name Sequence (5' to 3') Description Direction BGH-R ...terminator Reverse Other Common Sequencing Primers Name Sequence (5' to 3') Description Direction 3'AOX1... -
Chemogenetics Guide
TypeGuide...Effector Molecules (PSEMs). PSAM domains were first named for the mutations that allowed them to respond to...Liu, J., Inoue, K. I., Kumata, K., Seki, C., … Minamimoto, T. (2020). Deschloroclozapine, a potent and ... -
Molecular Biology Reference
TypeGuide...Ethanol) 25 µg/mL Hygromycin B 200 mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg... or G V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code Codons (RNA... -
Guide to Using Pooled Libraries
TypeGuide...experiments. As you might have guessed from their name, pooled libraries are supplied as a mixed population... -
Modular Cloning Guide
TypeGuide... below to find MoClo kits in our collection. Kit name Type PI Description MoClo Toolkit Plant Expression...