We narrowed to 417 results for: NUC

... Nicholas Thompson is a PhD Candidate studying Nuclear Science and Engineering at Rensselaer Polytechnic...

...and libraries using strategies related to other nucleases as well as inhibitory and activating approaches...

...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting...

...2007). All that is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled...

...VTA) but wish to stimulate VTA terminals in the Nucleus Accumbens (NAc; a primary target of VTA projections...

...References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441...

..."DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed...

...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Scientists have...

... 37 °C for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded...

...targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control...primers/probe (HEX/VIC) 1 µL 9 µL 900 nM, 250 nM Nuclease-free water 4 µL 36 µL Total Volume 16 µL 144 µL...

...Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal tracing Important considerations...

...membrane-bound subcompartments, like the mitochondria or nucleus, will likely require a harsher lysis buffer than...

...from a specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate...

...Ligation Dilute 5μL of annealed oligos with 45μL nuclease-free water and quantify the concentration (should...

...Restriction enzymes are naturally occurring bacterial endonucleases that recognize a large range of DNA sequences...

... the plasmid should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

...phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically...

... on a rocking platform. (Optional) Counterstain nuclei with 500 µL of 300 nM DAPI working solution for...

...the plasmids should also be propagated in an endonuclease negative E. coli strain such as NEB stable. ...

...use a HEK293 line stable expressing Epstein Barr nuclear antigen (EBNA). Safety Warnings HEK293 cells are...