We narrowed to 423 results for: NUC
-
TypeBlog Post...and libraries using strategies related to other nucleases as well as inhibitory and activating approaches...
-
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...Mizuno, and Atsushi Miyawaki. "Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...2007). All that is required is that the proteins/nucleic acids/lipids of interest are fluorophore labeled... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...VTA) but wish to stimulate VTA terminals in the Nucleus Accumbens (NAc; a primary target of VTA projections... -
University of Florida Serotype Testing Panel for the Eye and Brain
TypeCollection...Transduction and Volumetric Spread in the CNS. Mol Ther Nucleic Acids . Sep 15;8:184-197. PMID: 28918020 AAV6(dbY-F... -
Viral Production
TypeCollection...responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) as a control for... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming and pluripotency. Nature, 2006. 441... -
Lentivirus Plasmids
TypeCollection...are size (i.e., a limited number of basepairs of nucleic acid can fit into a viral particle) and toxicity... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post..."DNA targeting specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed... -
Handling Plasmids from Addgene - Purifying Plasmid DNA
TypeProtocol... 37 °C for 5 min. Note: Ribonuclease A (RNase A) is a pancreatic ribonuclease that digests single-stranded... -
TALENs for Endogenous Zebrafish Genes
TypeCollection... NIH-sponsored effort to provide gene-editing nucleases to the zebrafish community. Target Gene Name TALENs... -
Lentivirus ddPCR Titration
TypeProtocol...targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are used as a control...primers/probe (HEX/VIC) 1 µL 9 µL 900 nM, 250 nM Nuclease-free water 4 µL 36 µL Total Volume 16 µL 144 µL... -
Using AAV for Neuronal Tracing
TypeBlog Post...Retrograde AAV: Making the journey from axon to nucleus Rabies and neuronal tracing Important considerations... -
Technical Design of a Western Blot
TypeBlog Post...membrane-bound subcompartments, like the mitochondria or nucleus, will likely require a harsher lysis buffer than... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection... AiE0027m_3xC3 SYFP2 Ventromedial hypothalamic nucleus (VMH) Hypothalamus 230531 pAAV-AiE2150m-minBG-SYFP2... -
Tetracycline Inducible Expression
TypeCollection...repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA). In normal conditions... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...from a specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate... -
Genetic Code Expansion
TypeCollection...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...multiple guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate... -
Plasmid Modification by Annealed Oligo Cloning (with Protocols)
TypeProtocol...Ligation Dilute 5μL of annealed oligos with 45μL nuclease-free water and quantify the concentration (should...