We narrowed to 749 results for: REV

...Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge tubes, Neptune 3745... plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells into each...11(8):783-4 (Link opens in a new window) . Last reviewed on: September 9, 2023...

...Optogenetics for light control of biological systems. Nat Rev Methods Primers, 2 , 55. https://doi.org/10.1038/...engineer new optobiology tools and improve upon previous tools through directed mutagenesis. Find optical... CIB1 450 LARIAT Inhibits protein function by reversibly sequestering targets into large, optically assembled...domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester and release proteins from cellular ...protein that forms photolabile homodimers with slow reversal kinetics and a UV-B absorption profile 282 References...

...focus on my core responsibilities, which typically revolved around the broader vision rather than the day-...mistakes and learn from them. I started at my previous company by taking on responsibilities such as ...

...quantify them with biosensors! In this blog we will review the basics of biosensors and how to use viruses...building blocks. Physiological factors Unlike the previously described sensors, this class measures either...

...these pesky germs into useful tools. Here, we will review viral basics and the types of viruses used as biological...human subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV...

... other fields or writing grant proposals to be reviewed by interdisciplinary panels.  And highly successful...Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online with...

...science. Well, I’ll give you an example. At my previous company, Ginkgo Bioworks, our software development... the morning! 9:00 AM--Technical implementation review. The developers discuss which front-end and back-end...

...allowing for the targeting of sites that were previously unavailable. Figure 1: The PAM sequence...specific location, it must be accessible by Cas9. Our previous blog post The PAM Requirement and Expanding CRISPR...

...constructs could lower genome editing efficiency. One previous strategy for multiplexing gRNAs used the RNA nuclease...observed slightly higher mutation rates than they’d previously reported in protoplasts (15% and 9% of target...

... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...

...collaborator Some of these people you meet will review your papers or grants in the future, and don’t ... are talking to is really in your direct area.  Review the conference schedule and attendance list. Plan...

...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

... the photoswitchable protein podcast segment Reversible control of photoswitchable proteins, which change...study, the Woolley lab showed that these photo-reversible protein-protein interactions can control subcellular...

...Berkeley at the time and an eLife ambassador, had previous experience organizing open access events and grassroots...Scientific Outreach at Code Ocean and through her previous work at Center for Open Science and Sense about...

...studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these...

...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...induce genome editing. Such a system has been previously described for transcriptional activation/repression...

...combines these approaches to enable rapid and reversible recruitment of chromatin complexes to any genomic...regulation and subsequent mathematical modeling. Its reversibility allows precise and quantitative analysis of ...

...The pre-embedding immungold technique was used to reveal the hM4Di coupled to mCherry (top) or HA tag (bottom...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell...

...interdisciplinary field of biology known as bioelectricity is revealing the important role of bioelectrical signaling ...gradients for control of growth and form." Annual review of biomedical engineering 19 (2017): 353-387. PubMed...

..., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along...