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We narrowed to 741 results for: REV

Showing: 381 - 400 of 741 results

  1. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... a DNA double strand break (DSB) in the genome wherever the user chooses. After the initial cut, the next..., Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Nalgene, 565-0010 (for viral preps, if prep was not previously filtered) Microcentrifuge tubes, Neptune 3745... plus one 'no virus' control well). Perform a "reverse transduction" by seeding 50,000 cells into each...11(8):783-4 (Link opens in a new window) . Last reviewed on: September 9, 2023...

  8. To Each HIS Own

    Type
    Blog Post
    ...post-purification cleavage step to remove the tag in order to prevent it interfering with protein function. In contrast...with the protein of interest (Bornhorst 2000). To prevent this, wash steps often include low concentrations...

  10. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...studies (Martins et al., 2018 and Xing et al., 2010) revealed precisely that for breast cancer. What are tumor...malignant forms and noticed that myoepithelial cells prevented progression to malignancy, concluding that these...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...calculated fluorescent distances correlating with the previously established physical map. In comparing pairs ...induce genome editing. Such a system has been previously described for transcriptional activation/repression...

  12. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ..., see image above). We then select forward and reverse primers for each sample with the help of DNA-editing...prepared with the primer stocks for forward and reverse reactions, respectively, and then shipped along...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...to light as antibodies became commercialized, revealing the unreliability of antibodies. There are three...reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody crisis states...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...enter (and thus infect) cells, this safety feature prevents the virus from replicating and spreading in non-target...Types of Viruses Viral Vector Elements Tips for Preventing Viral Recombination  ...

  20. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....number with links to the plasmid pages for your review. We’ll also mail you a deposit kit with instructions...
Showing: 381 - 400 of 741 results