We narrowed to 404 results for: SEF

...for every experiment, at least one will likely be useful. Optimal gRNA selection With some CRISPR experiments...

... concentration of recovered DNA as this will be useful for the ligation step. 3. Ligate Your Insert into...

...antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data often ...

...'t like speaking, they don't feel like they get useful feedback, and they don't feel like they've made...

...through multiple focal planes of a sample may also be useful for providing fast imaging along with optical sectioning...

...dark state to a red fluorescent state make them useful for 2-color imaging with green PA-FPs. Table 2:...

... that can pass through cell membranes can prove useful for live-cell imaging. Experimental context and...

...be added one by one. This approach is especially useful for combinatorial analyses. For example, if you...

...alter the genome and chromatin architecture, it is useful to regulate the time in which dCas9 binding occurs...

...Sanger sequencing). See Wang et al. (2013) for a useful reference.  Q14: I have used 200ng to 500ng of ...

...continued replication of the virus. While this can be useful, if too many neurons are labeled, it can be difficult...

...Ptashne lab and we hope to see many more of their useful reagents shared via Addgene. Narayan S, et al....

...-on system (pTRE-Tight-MitoTimer) to show its usefulness in cell culture to report changes in mitochondrial...

...maintain high representation CRISPR CRISPR is a useful tool for genetic screening experiments, due to ...genomic locus. CRISPR pooled libraries are also useful for loss-of-function screens, gain-of-function ...different high-throughput experiments. They are useful for probing cellular pathways and mechanisms in...bacteriophages or yeast. Surface display libraries are useful for epitope mapping, protein engineering, and ligand..., and then cloned into a given vector. They are useful for gain-of-function screens, pathway discovery...genes are involved in a phenotype. They are also useful for drug resistance screens and noncoding RNA studies...

...transfect. However, note that this method is most useful for cell lines that you can transfect at high efficiency...types by using cell-specific promoters, which is useful for in vivo studies. While we don’t discuss CRISPR...population. Additionally, clonal populations are useful for determining if your edit is homozygous or heterozygous...

...gamma-retroviral vectors have many aspects that make them useful research tools in a variety of cell types and models...their integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term...

...gene therapy but has, however, proven to be very useful for designing and producing rAdV-based vaccines...Adenoviral backbone plasmid that lacks E1, E3, and E4. Useful for larger inserts. pAdTrack Class of shuttle/transfer...

...lentiviral vectors have many aspects that make them useful research tools in a variety of cell types and models...expression of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines ...

... constant regions of other antibodies. This has useful research applications, and is covered in the following...environmentally stable and more cost-effective. They are useful when epitope specificity is not a concern or in...

...version of SpRY Though these PAM-flexible Cas9s are useful for precision edits, they can be used for virtually... have been paired with CRISPR systems to create useful tools for large scale edits. PASSIGE ( P rime- ...