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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: SIM

Showing: 381 - 400 of 658 results

  1. Bioinformatics at Addgene

    Type
    Blog Post
    ...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...to two fold for the final titer value, which is simply due to the noise of the assay. In other words, ...). However, because purified AAV preps contain similar and low levels of background protein and chemical...

  4. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...

  5. Hot Plasmids - November 2023

    Type
    Blog Post
    ...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

  6. How to Write a Scientific Review Article

    Type
    Blog Post
    ...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I...present it all in one place to get a sense of the similarities and differences among proteins in the class....

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition of...

  12. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...

  15. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

  16. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

  18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

  19. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...
Showing: 381 - 400 of 658 results