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Showing: 381 - 400 of 612 results

  2. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...it’s a combination of many factors unique to this set of tools and the community they serve. As difficult...validating and using an AAV in a specific experimental setup. Teams across Addgene have worked hard to ensure...compared to other AAVs preps from 2016–2023. B) Inset showing distribution of only GCaMP6, jGCaMP7, and...

  4. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...your “gating strategy.” This series of markers are set up sequentially, from broadest to most specific marker...Subtypes of immune cells are often defined by a subset of the markers used to identify them, such as “...

  6. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...Tempurature: Analyze your expression conditions by setting up a small-scale expression experiment to test ...StrR, TetR) Expression of insoluble proteins  Rosetta2 (DE3)* Chloramphenicol (pRARE) Good for “universal... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  8. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...PMCID: PMC3748948.  Khetrapal, Varnica, et al. "A set of powerful negative selection systems for unmodified...expression of a gene with an antibiotic resistance cassette. This leaves a “scar” in the chromosome, sometimes...vector contains sacB, an chloramphenicol resistance cassette, and the R6K origin of replication to allow replication...pDS132 (Philippe et al., 2004): Replaced the sacB cassette with one of three toxins from the Khetrapal paper...medicine and clinical infectious diseases at the Massachusetts General Hospital where he is now an attending...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ... this skill that you could strengthen. The right set of transferable skills, coupled with your technical...know you are bringing this asset to your job, you can become a valuable asset yourself.     Fig... point of view and can’t understand why they’re upset, conflict resolution is likely not one of your strengths...

  15. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...large pan-cancer CRISPR-Cas9 gene dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467...nuclease-mediated off-target effects.” Further, large-scale datasets of hundreds of genetic screens using genome-wide...

  16. Antibodies 101: Validation

    Type
    Blog Post
    ...RNA expression of your gene of interest across a set of human tissues.  From this data, you see that there...and (2) large-scale proteomic and transcriptomic datasets already exist.   Example: You want to use an antibody...

  17. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...antibody is used to visualize the proteins. In this set-up, multiple secondary antibody molecules bind the...another great option to enhance resolution. In this setup, gels have an increasing range of acrylamide content...

  20. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...use, and robust in performance. In its natural setting, CRISPR-Cas9 is an acquired immune system in bacteria...Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette Knockin by CRISPR/Cas9-Mediated Genome Editing...
Showing: 381 - 400 of 612 results