We narrowed to 727 results for: ace

...amplified to add 5′ and 3′ homology regions to the adjacent fragment or vector. 5′ overhangs are generated...higher temperature at which Gibson assembly takes place may limit formation of secondary structures at the...

...can see from Figure 2 that determining where to place your gate based on the dot plot would be complicated...Conventional vs Spectral Flow Cytometry Beyond Surface Labeling in Flow Cytometry Resource on addgene....

...information, however, did not improve at the same pace as the quantity and quality of the information. ...down, and zooming out — with an intuitive user interface.   The team approached the issue from multiple...

...endeavor. Due to the COVID-19 pandemic, students will face a reduced supply of internships as a result of the...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

...adjustments in the physical organization of your workspace and your workflow can have a huge impact. Test... experiments at odd hours, used more secluded workspaces, or politely asked to not be disturbed when they...

...legends who not only survive, but thrive, in the face of some of Earth’s harshest environments: corrosive...out rapidly in warm weather. Psychrophilic enzymes face very similar issues when forced to express in mesophilic...

...focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific exploration, ... opened in August 2017.  This flexible teaching space offers STEM programming, workshops, and customized...

...outside your organization. The mentor in your own workplace is able to: Help you identify specific skills...can tap for email discussions. It is also a great place to practice your mentoring skills. Online mentoring...

...in recombinant AAVs (rAAVs) with maximum “trunk” space for genetic cargo. Typically, when researchers speak...dependent switches control gene expression via switches placed in your cell type of interest. Careful combination...

...communication, be it in your academic lab or your workplace, is an ongoing process. It is never really done...you try any of these approaches in your lab or workplace, let me know. If you have come upon any other ...

...Figure 1). This light-induced excitation can take place in a tissue culture dish but can also happen in ... motor cortex (M2). Since M2 is located on the surface of the brain, to deliver light to the channelrhodopsin-expressing...

...understand so that we could possibly develop pharmaceutical interventions that would inhibit IL-36 release...intensity-based signaling biosensors to monitor intracellular signaling,” says Hébert. Previous research has...

... RNA editing. Fast facts – PAM requirement: protospacer flanking sequence – A, U, or C. Best for: targeting...development and use. Fast facts – PAM requirement: protospacer flanking sequence – none. Best for: targeting...

... et al. (2018). Of course, preprints don’t replace journals and other modes of scientific communication...journal and share it more widely!  Not everyone embraces preprints as part of their scientific communication...

...entails will help keep you motivated when you’re faced with yet another hurdle in your research journey...will always be room to improve. The next time you face a problem, try to be mindful of how you work through...

...still do), so we are used to the idea of general interfaces and the freedom to test out lots of combinations...Specificity — coupling must happen in the right place in living systems when there are lots of other biomolecules...

...collaboration. The first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations...dedicated website to host all the materials in one place and better serve researchers at all levels of the...

... on size is ultracentrifugation. Sedimentation equilibrium analytical ultracentrifugation yields an affinity...

... Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make....9 kb stuffer sequence that can be removed and replaced with an shRNA sequence of your choice. More information...

... to undergraduate students (1, 2), but educators face significant challenges in designing suitable projects...complementation should only occur when galactose replaces glucose as the carbon source. Student results ...