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We narrowed to 949 results for: ache

Showing: 381 - 400 of 949 results

  1. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...

  2. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This... growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral... Rachel Leeson...

  3. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...YNP, it took some time to map a route that would reach all of the remote areas, including one hot spring...poles to collect water samples from a distance. At each collection site, we took GPS coordinates, water ...was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity with...

  4. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...assays, in vitro transduction, and silver stain approaches we show that there is no difference in purity...so excited? For starters, we believe that this approach can be adapted by vector cores and laboratories...investigated 3 serotypes it’s possible that this approach will be beneficial for a host of other AAV serotypes...serotypes; it will be exciting to test this approach with different capsids. Most importantly, because ...

  6. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with a different...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...

  7. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Below, you will find some easy talking points for broaching this subject with your institution’s tech transfer...Agreements. By default, your TTO must sign an agreement each time a material is being requested or deposited....Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering nearly all future ...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...higher copy numbers since the bacteria have just reached stationary phase, but the cells have not started...

  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ... great introduction to the financial world. It teaches you about selling products which is an important...

  10. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...you don’t have a formal connection, you can just reach out to start the conversation. Many researchers ...establishing collaborations, so it doesn’t hurt to reach out and ask if they would be willing to host you...available to directly help you during your visit. Reach out to your bench mentor (if you have one) to communicate...of their way to help you with your research and teach you new techniques. Show up on time (don’t make ...

  11. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will...community organizations and workforce projects to reach audiences beyond our plasmid depositors and requesters...

  12. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...with a valid email address).  One may also simply attach important references that serve to further characterize...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

  13. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

  14. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues that are necessary...fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When β10 and...FlipGFP were redesigned so that they are parallel to each other and thus can no longer fit with β1-9. They...dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence...

  15. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...workflows are limited by costly library prep, as each sample requires an individual library. The plexWell...DNA-barcoding step that uniquely labels DNA from each well of a 96-well plate. Then, samples from several...procedure, the quantities of library molecules for each sample remain highly normalized across a wide range...individual input DNA samples are brought in line with each other before proceeding to library prep. Even with...

  16. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

  17. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ...teams of individuals who enjoy and are experts in each of the individual tasks required to develop the ... project gets left undone or is duplicated. With each component of the project completed by an individual...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...that other people in the team grow and develop to reach their full potential in the organization. This will...

  18. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...activities – did you volunteer to do science outreach and teach children about science? Or perhaps you have...employer. For example, my experience in science outreach and writing was how I got my current job as a ...curiositycollider.org/) , which she co-founded, and the Outreach Manager for Science Borealis (hyperlink http:/...

  19. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko points...are probably because a PI does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager...message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building ...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

  20. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...all experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help...Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which...enzymes may better suit specific experimental approaches. One example is CasX, characterized by the Oakes...
Showing: 381 - 400 of 949 results