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...users have recently begun to develop CRISPR-based approaches for studying gene function in these systems. ...use in a fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived...tissue-types. By bringing together CRISPR-based approaches and organoid models, toolkits like this one provide...trigger seed germination of parasitic plants, this approach was also used to build and validate a ratiometric...

..., I reached out to Amy with exciting news. I managed to secure a virtual summer program, teaching me the.... A capstone project is a major project given to each intern to complete and present on.    Figure...report shared my work by breaking down the process of each day, my background, observations, etc. On my final...

...University and entered a new role here at Addgene as an Outreach Scientist. I used to spend my days (and often ...This is particularly true in my position as an Outreach Scientist where I spend a large amount of my time...communicator that got me here! Thus far being an Outreach Scientist at Addgene has been awesome. The process...researchers gain doing PhDs (i.e. technical writing, teaching, presenting, analytical thinking, time management...

...chromosomal DNA, but they still rely on the host machinery to make additional copies. ori sequences are generally...lower temperatures and allows the replication machinery room to come in and get busy making copies.   ...incompatible because they will compete for the same machinery, creating an unstable and unpredictable environment...higher copy numbers since the bacteria have just reached stationary phase, but the cells have not started...

...disease front. Viruses survive by hijacking the machinery of the cells they infect. Once they enter a cell...delivered to the cell. The cell then uses its own machinery to replicate the viral genome and proteins. This... growing number of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral... Rachel Leeson...

...surface. Their toolkit includes a set of plasmids each containing an anti-GFP nanobody fused with a different...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with specific...Sontheimer and his group from the University of Massachusetts Medical School have developed a group of plasmids...

...assays, in vitro transduction, and silver stain approaches we show that there is no difference in purity...so excited? For starters, we believe that this approach can be adapted by vector cores and laboratories...investigated 3 serotypes it’s possible that this approach will be beneficial for a host of other AAV serotypes...serotypes; it will be exciting to test this approach with different capsids. Most importantly, because ...

...a smaller RNA tag, are less prone to photobleaching, and achieve better signal in vivo as compared to ...the second containing the growth promoting domain. Each half was fused with one of the LOVTRAP domains. ...dCas9 fused to cytidine deaminases like APOBEC. To achieve this they created a lentiviral vector system that...

...another method Transfection and transduction seem to achieve similar outcomes, so why and when should you opt...Second, what is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo...work is that you can swap out your virus type to achieve different biological outcomes. For example, some...targeting of that virus, which can’t be easily achieved with alternative methods. Ready, set, transduce...

... unexplored. Using new single-cell sequencing approaches, the lab was able to eliminate the need for axenic...YNP, it took some time to map a route that would reach all of the remote areas, including one hot spring...poles to collect water samples from a distance. At each collection site, we took GPS coordinates, water ...was separated into smaller samples of 5-10 cells each for sequencing, allowing for lower complexity with...

...Below, you will find some easy talking points for broaching this subject with your institution’s tech transfer...Agreements. By default, your TTO must sign an agreement each time a material is being requested or deposited....Reception and Master Deposit Agreements, your TTO signs each agreement just once, covering nearly all future ...University of California, Irvine University of Massachusetts, Worcester  University of Nebraska Medical Center...

...Presentation Experience”, “Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me... business friendly CV that would focus on my achievements, capabilities, courses and office skills. I ... great introduction to the financial world. It teaches you about selling products which is an important...

...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...ratio will need to be empirically determined for each new batch of 1 mg/mL PEI prepared. There may be ... used, volumes, pH adjustment etc. Consequently, each batch needs to be validated and the best ratio of...

...cell/DNA mixture on ice for 20-30 mins. Heat shock each transformation tube by placing the bottom 1/2 to...enough colonies for your next step. Remember that each of these shortcuts will reduce the efficiency of...cuvettes. Follow the manufacturer's instructions for each. I got no transformants. What went wrong? Check ...

...Place a sterile poly-D-lysine coated coverslip in each well of a 24-well cell culture treated plate. Seed... aspirate the media from the 24-well plate. Wash each well with 500 µL of PBS, remove the wash, and dispose...dispose of it in an appropriate waste container. Fix each well with 500 µL of cold 4% paraformaldehyde in ...

...while the alcohol used in the staining process bleached another, Klebsiella pneumoniae. After realizing...safranin, as a counterstain to help visualize the bleached bacteria. The chemistry behind Gram’s crystal ...Society for Microbiology (2005). https://asm.org/getattachment/5c95a063-326b-4b2f-98ce-001de9a5ece3/gram-stain-protocol...

...you don’t have a formal connection, you can just reach out to start the conversation. Many researchers ...establishing collaborations, so it doesn’t hurt to reach out and ask if they would be willing to host you...available to directly help you during your visit. Reach out to your bench mentor (if you have one) to communicate...of their way to help you with your research and teach you new techniques. Show up on time (don’t make ...

...guides – including an excellent series of posts by Joachim Goedhart on the Addgene blog – outlining various...with a valid email address).  One may also simply attach important references that serve to further characterize...better way to store and represent within-study photobleaching comparisons.  We very much appreciate feedback...Techniques - Which is best for me? A Practical Approach to Choosing the B(right)est Fluorescent Protein...

...2) β-strand 10 (β10), and (3) β-strand 11 (β11). Each part contains components and residues that are necessary...fluorescence. When these parts are separated from each other, the protein cannot fluoresce. When β10 and...FlipGFP were redesigned so that they are parallel to each other and thus can no longer fit with β1-9. They...dynamic range and high brightness. This reporter achieves approximately 100-fold increase in fluorescence...

...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will...community organizations and workforce projects to reach audiences beyond our plasmid depositors and requesters...