We narrowed to 494 results for: control
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TypeBlog Post...FRET measurements and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation...
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Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...MTA) approval Send plasmids to Addgene Quality control In this post, we’ll explain how to use our deposit... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...living cells where methylation of these sites was controlled, the reporter consistently showed significantly... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...conjunction with activator or repressor domains to control gene expression. Base editors can help you make... -
Neurodegeneration Research Collection
TypeCollection...neurons in the substantia nigra, one of the movement control centers in the brain. The cause of this neuron ...Symptoms include changes in behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty... -
Lentivirus Plasmids
TypeCollection...pLVTHM 2nd EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter. Didier Trono 11651... -
Technical Design of a Western Blot
TypeBlog Post...stains as loading controls: An alternative to high-abundance single protein controls in semi-quantitative... -
Guide to Using Pooled Libraries
TypeGuide... Perform Next Generation Sequencing (NGS) on a control sample (no selection) In parallel, apply library...cells Compare NGS results between experimental and control cells Generate a list of gRNAs that disappear with...selection while the other serves as a non-selected control. These two populations are then sequenced using...Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post... lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post...Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...more sensitive to trimethoprim when compared to control gRNA treated or untreated bacteria. Had the folA... -
Pooled Library Amplifications
TypeBlog Post... CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled Libraries Resources on Addgene.org Addgene's... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...action potentials, muscle contractions, and the control of voltage gated ion channels all depend on transmitting... -
Addgene's Cancer Collection Pages Connect the Research Community
TypeBlog Post...opposite task, keeping cells from dividing out of control. One may compare the roles of proto-oncogenes and...same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation... cellular level there is a universal theme - uncontrolled cellular proliferation. We have appreciated ...appreciated for some time the causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post... enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...of all distributed plasmids as part of quality control, but does not perform functional tests. The newest...