We narrowed to 494 results for: control

1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   ...editing in their livers, while non-injected and control gRNA injected mice had no detectable editing. Additionally...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   Blog Post

   ...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...

3. Plasmids 101: Knockout/Knock-In Plasmids

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   Blog Post

   ...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...

4. Making CRISPR Plasmids Using Fragmid

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   Blog Post

   ... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

6. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

7. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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   Blog Post

   ...conjunction with activator or repressor domains to control gene expression. Base editors can help you make...

8. Technical Design of a Western Blot

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   Blog Post

   ...stains as loading controls: An alternative to high-abundance single protein controls in semi-quantitative...

9. Guide to Using Pooled Libraries

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   Guide

   ... Perform Next Generation Sequencing (NGS) on a control sample (no selection) In parallel, apply library...cells Compare NGS results between experimental and control cells Generate a list of gRNAs that disappear with...selection while the other serves as a non-selected control. These two populations are then sequenced using...Screening Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens New Tool for...

10. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    Blog Post

    ...Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out...

11. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    Blog Post

    ...genome, it is not expressed as it is under the control of a rhamnose-inducible promoter. The resolution...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016...

13. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:...

14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ... lentiviral plasmid to express ubiquitin under control of a CMV promoter. Starting with two search terms...

15. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post

    ...more sensitive to trimethoprim when compared to control gRNA treated or untreated bacteria. Had the folA...

16. Pooled Library Amplifications

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    Blog Post

    ... CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled Libraries Resources on Addgene.org Addgene's...

17. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...action potentials, muscle contractions, and the control of voltage gated ion channels all depend on transmitting...

18. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post

    ...opposite task, keeping cells from dividing out of control. One may compare the roles of proto-oncogenes and...same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation... cellular level there is a universal theme - uncontrolled cellular proliferation. We have appreciated ...appreciated for some time the causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes...

19. 5 Tips for Troubleshooting Viral Transductions

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    Blog Post

    ... enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw...

20. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...of all distributed plasmids as part of quality control, but does not perform functional tests. The newest...