...FRET measurements and serve as a type of positive control. In the case of an unfavorable donor-acceptor orientation...

...MTA) approval  Send plasmids to Addgene  Quality control  In this post, we’ll explain how to use our deposit...

...resistance gene using Cre recombinase. If GFP is under control of an endogenous promoter, you can use expression...

...polymerase encoded in the E. coli genome (usually controlled by IPTG). For this IPTG-induced expression to...

...editing in their livers, while non-injected and control gRNA injected mice had no detectable editing. Additionally...

...Ming G-L, King JR, Song H, Sweatt JD (2013) TET1 Controls CNS 5-Methylcytosine Hydroxylation, Active DNA...

...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

...conjunction with activator or repressor domains to control gene expression. Base editors can help you make...

...to bind to an unallocated codon. Therefore many controls must be used to make sure that these conditions...conditions are true. Always express first with a control reporter gene – GFP for E. coli or mCherry-GFP for mammalian...

...stains as loading controls: An alternative to high-abundance single protein controls in semi-quantitative...

... CRISPR Preparing Pooled Libraries NGS Quality Control for Pooled Libraries Resources on Addgene.org Addgene's...

...action potentials, muscle contractions, and the control of voltage gated ion channels all depend on transmitting...

...opposite task, keeping cells from dividing out of control. One may compare the roles of proto-oncogenes and...same result – uncontrolled movement of the vehicle or, in the case of cancer, uncontrolled cell proliferation... cellular level there is a universal theme - uncontrolled cellular proliferation. We have appreciated ...appreciated for some time the causes of this uncontrolled proliferation, in particular the roles of proto-oncogenes...

... enzyme digest and compare it to some reliable controls. 2. Viral stocks may be sensitive to freeze thaw...

...of all distributed plasmids as part of quality control, but does not perform functional tests. The newest...

...of the Deposit Process Learn How We Do Quality Control at Addgene Resources on Addgene.org Join the ...

...more sensitive to trimethoprim when compared to control gRNA treated or untreated bacteria. Had the folA...

...adenoviral delivery of CRISPR/Cas9. Journal of Controlled Release 298:128–141 . https://doi.org/10.1016...

...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:...

...Sequencing Analyst intern. As a member of the Quality Control team, I help make sure the plasmids that go out...