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... the limitations is the size of GFP: ~240 amino acids or about 28 kDa. GFP and its homologues have a beta-barrel...glycines, serines and other small, non-aliphatic amino acids. However, long linkers increase the chance that ...fusions, our experience is that a linker of 4 amino acids (PVAT, when we use the AgeI site in the multiple...Download the fluorescent proteins 101 eBook Read our plasmids 101 blog series Resources on Addgene.org Browse...

...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...

...refers to the different protein compositions of AAV capsids, which determine how a virus enters the cell. Different...Manfredsson, F. P. (2017). Rationally Engineered AAV Capsids Improve Transduction and Volumetric Spread in the... the CNS. Molecular Therapy - Nucleic Acids, 8, 184–197. https://doi.org/10.1016/j.omtn.2017.06.011 Korneyenkov...AAV-Mediated Gene Transfer. Molecular Therapy - Nucleic Acids, 8, 13–25. https://doi.org/10.1016/j.omtn.2017.03.009...

...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...mRNA can boost efficiency over transfecting with plasmids in some cell lines, most likely due to DNA toxicity...

...to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While this strategy greatly...Recombinant AAV Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential....2020.618020 Additional resources on the Addgene blog Plasmids 101: FLEx Vectors Important Considerations When... ready to use AAV vectors at Addgene. Find AAV plasmids to use in your experiments. ...

...for mammalian RNAi Browse all plasmids for RNAi Check out Addgene's plasmids and resources for plant biology...harvested instead of microscopic isolation. The plasmids required to conduct an APT-based RNAi experiment... target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert Orr...

...detect various targets, such as proteins, nucleic acids, or glycans. Fluorophores can also be covalently...should have high affinity and specificity to nucleic acids, with minimal background staining. Additionally,...cell and protein analysis products. You can find plasmids deposited by Promega here.    References and ...Self-Labeling Protein Tags Fluorescent protein plasmids and resources A practical approach to choosing...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged... protein of interest or using non-natural amino acids to directly incorporate a fluorophore in your protein...self-labeling tag systems Tag Size (amino acids) Ligand Commercial ligands available Ease ...Various Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications...

...Resources at Addgene.org Find CRISPR Plasmids Find Synthetic Biology Plasmids Browse our CRISPR Guide Pages ...our CRISPR featured topic page Check out our Hot Plasmids Podcast Listen to our interview with synthetic...

...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...to check out the Trono Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to...

...other scientists save resources by sharing plasmids Find plasmids at Addgene ...With a collection of over 70,000 plasmids, kits, and viral service items we employ dozens of pieces of...

...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on...Resources on the Addgene Website Find Cepko Lab Plasmids at Addgene ...

...Addgene.org Find Fluorescent Protein Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization...

...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...for treating genetic diseases. In our summer Hot Plasmids post, we covered the Liu lab’s first demonstration...Addgene.org Read the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection...

...exhibitor booth in order to help scientists find the plasmids they need for their research, show labs how they...about all the amazing research being done with plasmids. I heard an impressive talk about the use of optogenetics...and resources by storing and distributing their plasmids with the scientific community. All of this speaks...

...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

...distributed 165,000 plasmids, including 27,000 CRISPR plasmids and 6,000 biosensors plasmids. Our most exciting...

...put to such great use. It’s just one of the many plasmids being distributed to and accelerating research...Knockouts Read Some Common Ways to Deliver CRISPR Plasmids to Mammalian Cells Resources on the Addgene Website...Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries ...

...about other Protein Tags in our Plasmids 101 Series. Get the Inntag Plasmids Tag endogenous proteins with... in a scientist’s toolbox. As we’ve covered in Plasmids 101, tags can help you determine localization ...

...ERK, or a backbone to use for custom KTR design, plasmids are available from Addgene! References Kudo, ...protocols, 13 1, 155-169. PMID: 29266096. Find the KTR plasmids here! Additional Resources on the Addgene Blog...Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors ...