We narrowed to 470 results for: mag

1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...selection of sybodies against membrane proteins. Image from Zimmermann et al., 2018. Ribosome display...

2. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...questions about our quality control process! The images in this blog post were created using Snapgene and...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

   ...using FP timers, researchers can look at a single image of a cell and understand how protein localization...

4. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...potential to be incredibly useful in therapeutics. Imagine deleting a problematic copy of a gene that causes...

5. The AAVantages of AAV in CRISPR Screens

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   Blog Post

   ...Aryee, M. J., Joung, J. K., Breakefield, X. O., Maguire, C. A., & György, B. (2019). High levels of AAV...

6. Common Injection Routes in Mice

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   Blog Post

   ...representation of common injection routes in mice. Image created with biorender.com.   Needle techniques...

7. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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   Blog Post

   ...tissues, stem cells serve to replenish/replace damaged cells that no longer function adequately [1]. Stem...

8. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ...different genetic loci within a single cell. One might imagine transfecting cells with both the nuclease-positive...

9. Addgene's Cancer Collection Pages Connect the Research Community

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   Blog Post

   ...mechanism. When a cell or its genetic material is damaged beyond repair, that cell will most often commit...

10. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    .... Spiking your media with supplements such as magnesium salts, buffering agents, and/or supplying additional...

11. Genome-wide Screening Using CRISPR

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    Blog Post

    ...genes that are essential for a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712...

12. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ...time for using your plasmids instead! All BLAST images are modified screen shots from the NCBI BLAST website...

13. SciComm: Taking Science to Elementary and Middle Schools

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    Blog Post

    ...which is likely a career path none of them had imagined for a scientist. I think that establishing this...

14. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...-1 locus. Figure 1: Schematic of enCHIP. Image from Fujita and Fuji, 2013. Prior to the development...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...outshines FRET — BiFC experiments only require that you image and measure the fluorescence of a single FP. Your...

16. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    Blog Post

    ...more immediate and dire than Zimmer could have imagined. Does this detract from the quality of the book...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...rolling mean gave us a crude insight into the magnitude of the effect COVID-19 had on our data. Because...

18. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...applicable to those positions you have already held. Imagine this situation: You’ve spent hours scouring a company...

19. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    Blog Post

    ...amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed PMID: 25307933...

20. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...work for all bacteria; may cause plasmid/host DNA damage. Choosing an antibiotic resistance Choosing the...