We narrowed to 760 results for: mpo
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TypeBlog Post...for plasmid quality control are: To identify important plasmid features To align NGS sequencing results...Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all...
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Understanding GPCR Signaling Using TRUPATH
TypeBlog Post...They further used TRUPATH to analyze a panel of compounds across the transducerome on a single GPCR, showing... the TRUPATH system. Conclusions Although the importance of GPCR pathways is well known and, as a result... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post... Timing Optogenetics offers a high degree of temporal resolution as receptors can be turned on and off... meet these requirements. TL;DR If precise spatiotemporal control of a small subset of neurons is necessary... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS...in high throughput screening for agrochemical compounds that have the potential to regulate the parasitization... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post... with my teammates acted as my safety net and trampoline all at once. I was able to do something that ...achieved my goal of helping everyone understand the importance of using analytics to make decisions on content... -
Year of the Camelid: Antibody Style
TypeBlog Post... raise awareness of the economic and cultural importance of these animals to human populations around ...through biomedical research. The Camelid family is composed of camels, llamas, and alpacas (and their undomesticated... -
Networking and Inspiring Young Scientists at Freshers Fairs
TypeBlog Post...throughout the year, including a Christmas Chemistry symposium and a career night. The society also visits high...engaging with young scholars. We believe it’s very important to develop a network of fellow scientists that... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...therapy - avoiding the use of viruses is also important for social and regulatory reasons. 1.) The transposon... in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required for... -
Management for Scientists: Managing vs. Leading
TypeBlog Post...” instead of levels because it emphasizes the importance of these very different roles. At any stage in...doing, managing or leading. All three roles are important to accomplishing any goal or finishing any project... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post... RNA templates to DNA but suffer from several important shortcomings including low processivity (ie. the... restricted spatial tagging), to confirm the composition of proteins found at the telomere, and hope it... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant lab ...every day, and this crisis has highlighted the importance of access to quality materials and data. Addgene... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...the Cre recombinase, are used to spatially and temporally control transgene expression. Lox sites are directional...sites lets researchers control the spatial and temporal activation of a transgene. Figure 2: FLEX... -
Intro to Our Summer SciComm Series!
TypeBlog Post...through manuscripts, seminars, or posters. It is important to note that it is the actual communication itself...quickly! The question The first, and most important question, you should ask for any science communication... -
Another Pathway into Cells: iTOP
TypeBlog Post...of their luciferase assay, they concluded that components in the buffer used for protein purification must...indicating a 70% frequency of biallelic mutations. Importantly, hESCs retained their stemness, indicating that... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...mentoring relationship really productive, the most important step you can take is to set goals to work on together...to help a Mentee reach goals. One of the most important contributions made by a Mentor is to help the ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post... the vascular wall. ApoB, the main structural component of atherogenic lipoproteins, shuttles lipoproteins...hypothesizes that this may be due to different lipid compositions of C. elegans membranes, which might beneficially... -
Creating accessible biology activities in schools with BioBits
TypeBlog Post...kits based on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free...FD-CF reactions affordable to many schools. Most importantly, experiments for FD-CF reactions can be fun and... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post... other hand absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm...equipment, and expected DNA concentration. It’s important to consider the advantages and disadvantages of... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...session you’re presenting is eye contact. “It’s important to keep your video on and look into the camera...networking within the app. For example, the NeuroSymposium had virtual tables that you can join to connect... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...physiologically-relevant systems, to catch all the important interactions. It’s also important to determine if the human interactors...