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Showing: 381 - 400 of 783 results

  2. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: a Cas9 nickase 101 blog post.  On the twelfth day of CRISPR, Addgene gave to ...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...model generation simpler, as described in an earlier post on generating CRISPR mouse models. For simple knockout...M. Quadros for assistance with editing this blog post. References Yang, Hui, et al. "One-step generation...Resources on the Addgene Blog Read Our Previous Post on CRISPR Mouse Models Learn about Homology Directed...

  4. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...where you can thrive and do your best work. This post is focused on choosing a lab, but almost all of ...blunders, setting target up to fail, shifting goal posts without telling the target Warning signs while ...Learn about careers in science communication Read posts on mentoring for scientists Resources on Addgene.org...around and accessible for advice and input? Will a postdoc or lab manager be your actual mentor? Is there ...attending career events or being involved in a grad or postdoc association? Will you learn how to run a lab if...a way to grow and learn. Graduate students and postdocs in particular must care about this. The training... than one lab when you choose a grad school or postdoc lab. Having a comparison can be very helpful in...

  5. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...are being up- or downregulated in a system, detect post-translational modifications, quantify protein levels...immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications In addition to protein...abundance, westerns detect changes to a protein’s post-translational modifications such as phosphorylation...

  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger, Addgene Advisory...genes involved in a phenotype of interest. This blog post will discuss differences between these approaches...CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org Brush up on Your CRISPR...

  7. Antibodies 101: Validation

    Type
    Blog Post
    ...results. But what is appropriately validated? This post will go over some of the general considerations ...antibody? Check out our Selecting an Antibody blog post! Learn more about Antibodies and their many applications... Antibodies 101: Introduction to Antibodies blog post!  Resources on Addgene.org Find plasmids encoding...

  8. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...This blog post was originally written by Caroline LaManna, published Mar 8, 2016. The updated and expanded.... For a more detailed review, check out our blog post: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems...prior to irradiation with UV light (365nm) and that post-UV exposure it showed cleavage activity similar ... the Addgene Blog Read Other Optogenetics Blog Posts Read Our Primer on Optogenetics by Derek P. Simon...

  9. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: updated CRISPR101 blog posts!🎵 On the 4th day of CRISPR, Addgene gave to me:...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Plasmids: Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale,... Keyence BZ-9000 fluorescence microscope 8 hours post-pulse. The distinct differences of red-to-green ...phosphorylation is one of the most abundant forms of post-translational modifications in cells and research...see the detailed plasmid kit page or read our blog post: Quick, Versatile Plant Transgenesis with GreenGate...Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...Addgene's First 10 Years Addgene’s Top 10 Blog Posts from 2014  ... in the presynaptic region of one neuron and postsynaptic region of a different neuron, come into proximity...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    ...researcher at Lunenfeld-Tanenbaum Research Institute, posting about Addgene’s 20th anniversary on Twitter. In...!” — Julien Béthune, Professor at HAW Hamburg, posting about his Blue Flame Award on Bluesky. In 2016,...

  12. To Each HIS Own

    Type
    Blog Post
    ...required the incorporation of a cleavage site and a post-purification cleavage step to remove the tag in ...information, sign up to receive alerts for antibody posts from our blog!  Resources and References  Resources...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme. Fast facts – PAM requirement...cleavage). You can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement...

  14. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...degradation of important cellular RNA molecules with post translational modifications. One of the most common...picked your perfect guide; now check out our blog posts on choosing the best Cas9 enzyme to pair with your...

  15. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... an in-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review ...the other a crRNA array plus a GFP tag. Four weeks post-infection, there was strong expression of Cpf1 and...

  16. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...science career related blog posts Read our science communication blog posts Resources on Addgene.org ...widely. The way you communicate your science at a poster is going to vary depending on the conference you... in an academic lab setting, where student and postdoc turnover happens regularly and it behooves everyone... words like this when starting rotations or my postdoc would have simplified the first weeks or months...you discover during grad school or during your postdoc that you enjoy and have a propensity for cross ...

  18. What's New in CRISPR - November 2021

    Type
    Blog Post
    ... subscribe to our CRISPR blog content.   In this post we will cover: Miniature CRISPR-Cas systems SEND...Download the CRISPR 101 eBook Find our CRISPR 101 blog posts Read our CRISPR cheat sheet Resources on Addgene.org...

  19. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...suggests changes in RC2H1 protein expression and post-transcriptional regulation of SM1v1-containing RNAs... by IRE binding proteins, IREB1 and IREB2, which post-transcriptionally regulate iron metabolism pathway...

  20. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...continue where the guide left off - namely, a blog post devoted to the other affinity reagents.  Affinity... antibody?  That is the question… or is it? This post isn’t meant to convert anyone to downsize their ...
Showing: 381 - 400 of 783 results