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TypeBlog Post...projects. The idea behind the platform was simple: any person working on COVID-19 could file a request on our...collaborations beyond COVID-19 One year on, our biggest lesson running the platform has been the realization that...
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25 Work From Home Tips for Scientists
TypeBlog Post...Taking Care of Your Behavioral Health. Work on personal development Take the myIDP (individual development...a challenging period for everyone for numerous reasons, and you shouldn’t expect yourself to be as productive... -
Cultivating Community Science at BosLab
TypeBlog Post...and some home-made equipment. It was humble in comparison to the labs you’d find in Kendall square, but...at BosLab Not all of our members start out with personal projects in mind. Thus, to encourage more involvement... -
How to Lead a Great Meeting
TypeBlog Post...Project Meetings: You do not have to include every person who could potentially be impacted by a project....team meetings, provide agenda sections for each person to add a bullet or two about what they are working... -
Advice for Scientists Starting a Lab
TypeBlog Post...." She also recommends to seek out advice from seasoned PIs who can offer support. “It is an exciting ...Email. Nothing prepared me for what a PI without a personal assistant has to deal with. My inbox currently... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...never picking up the phone you don’t get to know a person. These interactions could either help you in your...an MBA in finance and a B.S. in accounting from Babson College. He is a certified public accountant (CPA... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post...precision genome editing workflow. Digital PCR comparison to other validation methods As mentioned, these...PMC3773023. 7. Vouillot, L., Thélie, A. & Pollet, N. Comparison of T7E1 and surveyor mismatch cleavage assays... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog Post...inactive state, and cheap. Unless there’s a specific reason you can’t use it for your ELISA and Western, I’... Rachel Leeson... -
From Lab Bench to Leadership: Transitioning into Management as a PhD
TypeBlog Post...disengagement. By tailoring my leadership approach to each person, I was able to create a more productive and cohesive...similar transitions can provide you with insights, lessons from their experiences, and practical advice for... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog Post...classification system, CasID, to help facilitate direct comparison of different enzymes. CasPEDIA is meant to be...isn’t always the winner!). Fig. 3 – Comparison of different Cas enzymes using CasPEDIA. Adapted... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post...For safety and production reasons, multiple plasmids with many components are used to package lab grown...will discuss them together. Gene expression The reason for most viral experiments is to deliver a genetic... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable base editing of ...KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu ... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post...mesophilic systems are the psychrophilic enzymes. The reason behind this isn’t really hard to guess. Think about...for all the Indian iGEM teams to interact with personnel from the industry and brainstorm with each other... -
Early Career Researcher Toolbox: Social Media for Scientists
TypeBlog Post...started working in a new lab and I was the only person in the lab. When I saw a request for an Addgene...scientific conference, whether you are attending in person or monitoring that conference via social media.... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...the first author of this article, Dr. Douglas Richardson, recently contributed a relevant post to the ...Addgene blog. Find the SRpHi Biosensors here. Richardson et al. Nat Communications. 2017. PubMed PMID:... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...fluorescent protein can be identified by a head-to-head comparison of several fluorescent proteins of the same spectral.... In figure 1 we provide an example of such a comparison between cyan fluorescent proteins using widefield... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson... -
Antibodies 101: Affinity Reagents
TypeBlog Post...entirely new affinity reagent. Fig. 1: A comparison of an antibody (left) to several alternative ... Rachel Leeson...