We narrowed to 470 results for: Lif

1. Transferable Skills Guide: Managing a Team

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   ...undoubtedly negatively impact your work and your life. It’s up to you to decide which one you want to...

2. Fluorescent Proteins 101: Fluorescent Protein Timers

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   ...protein or protein fusion. This one protein method simplifies cloning and makes your experimental setup less...

3. Truncated gRNAs for Regulating Gene Expression

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   ...Lance-Byrne is a graduate student at the University of California, Santa Cruz, where her research is focused on...

4. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   ...Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene blog...

5. Hot Plasmids: Summer 2025

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   ...Top: TIRF-SIM imaging of COS-7 cells expressing LifeAct fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification...

6. Hot Plasmids: Winter 2025

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   ... an enhanced pU6cm promoter, viral replicon amplification of cargo, and heat treatment.  Figure...

7. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...critical to the experiment, they definitely made my life easier!  In this post, I’ll share some AAV production...

8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   ...bees when it comes to fruit flies Drosophila start life as fertilized eggs that develop into embryos and...

9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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   ...I chose the 96 most useful things for day-to-day life in a plant lab, so it includes everyone’s favorite...

10. Capturing a Moment with Marc Zimmer’s “The State of Science”

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    ...future rabbit holes. For the non-scientists in your life, the book may be the introduction they need to grasp...

11. DNA Purification Without a Kit

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    ...directly into a PCR buffer, where DNA or RNA can be amplified. Compared to a commercial magnetic bead-based ...

12. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...

13. Summer SciComm Series: Modes of Communication

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    ... drive the kinds of communications you create. Wildlife biology and ecology can make for beautifully compelling...

14. SciComm: Taking Science to Elementary and Middle Schools

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    ...becomes a scientist. I gave a timeline of my science life, starting back to Mr. Kelley, my 7th grade biology...

15. Isolating Genomic Regions of Interest with the CRISPR System

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    ...array is modulated by DUX4-induced MBD3L proteins." Elife 7 (2018): e31023. PubMed PMID: 29533181. PubMed ...

16. Tips for arabidopsis transformation

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    ...plant genome using CRISPR/Cas9 Using pSiM24 for simplifying plant genetic engineering Using arabidopsis in...

17. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ...et al. "A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

18. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    ...B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1 (2017...

19. AAVs in Retinal Gene Therapy

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    ...and not another genetic disease more prevalent or life-threatening (cystic fibrosis, hemophilia, muscular...

20. AAVs CREATed for Gene Delivery to the CNS and PNS

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    ... creates a template that’s suitable for PCR amplification using pre-designed primers. By using this library...