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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 650 results for: CHI;

Showing: 601 - 630 of 650 results

  5. Lab to Office Culture Shock

    Type
    Blog Post
    ...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...

  11. Bioinformatics at Addgene

    Type
    Blog Post
    ... programs to a uniform language.  The modern architecture of Nextflow included built-in support for containers...

  21. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... introduce a frame-shift mutation. This can be achieved using a restriction enzyme that recognizes a unique...

  25. CRISPR Guide

    Type
    Guide
    ...Purify Genomic Regions The enChIP (engineered DNA-binding molecule-mediated ChIP) system, developed by the...the DNA, to create a DSB enChIP En gineered DNA-binding molecule-mediated ChIP , using a tagged-dCas9+gRNA...the ways the CRISPR components are leveraged to achieve specific goals, such as targeting multiple genomic...HDR) pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing...gRNAs into a single plasmid — though some have achieved targeting in the double digits. These multiplex...nickase system is utilized in many CRISPR systems to achieve specific genetic edits. The dCas9 molecule binds...or G, Y = C or T SpRYc - SpRY PAMs plus NNG; a chimeric version of SpRY Though these PAM-flexible Cas9s...

  29. Antibody Guide

    Type
    Guide
    ... Special considerations One type of ChIP assay, called native ChIP, does not use cross-linking and instead...accessible to antibodies. Detection of sdAbs is usually achieved by conjugation of a signaling molecule directly...can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to distinguish...detect simultaneously. Conjugation The process of attaching signaling molecules to an antibody is done through...Immunoprecipitation (IP) Chromatin immunoprecipitation (ChIP) Visualization - used to visualize protein location...either X-ray film in a darkroom or an image capture machine. Special considerations The primary antibody must...immunoprecipitation (IP) and chromatin immunoprecipitation (ChIP) allow you to isolate specific proteins or protein...
Showing: 601 - 630 of 650 results