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We narrowed to 778 results for: Dos

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Showing: 601 - 630 of 778 results

  7. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...off"). These Cre-off switches may be referred to as DO (Double-floxed Orientation) and could be used to ...

  11. A Look at Addgene's QC Process

    Type
    Blog Post
    .... Fun fact! Some older plasmids in our collection do not have a full sequence, as they were deposited ...

  13. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ... provided you with a few ideas to consider if you do run into a roadblock and encourage you to check out...overcome this issue, we recommend using a range of doses of lentivirus when transducing a target cell line...transduction experiment. Media choice, reaction vessel, dose of virus, volume and timing of transduction will...

  14. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...Despite their advantages, mammalian expressions systems do require demanding cell culture conditions compared...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...been used to treat 10 infants with this disease. To do so, their T-cells were grown in vitro, their mutations...to be untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the...

  24. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool from the past... are turned on or off in a given area and the can do so in a very specific, precise manner. Previously... are bound to the coding region are displaced and do not rebind because they are transported back to the...their quest to understand lncRNAs: What can lncRNAs do by themselves? The Rinn lab has developed a novel...regulation has roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell ...reticulum (ER), mitochondria, cytoskeleton, and endosomes. These fusion proteins can act as markers for ...Microtubules mCherry-alpha-tubulin Early Endosome Vacuolar Compartment mCherry-Rab5 BFP-Rab5 ...

  25. Bacterial Expression Systems

    Type
    Collection
    ... 2025. Catalog items are updated more frequently. Do you have suggestions for other plasmids that should...

  27. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...buffer as the one in the gel box (do not mix different buffers and do not use water). Microwave for 1-3...instructions on how to do this, visit the Gel Purification page. Tips and FAQ How do you get better resolution...min until the agarose is completely dissolved (but do not overboil the solution, as some of the buffer ...to the running buffer when you run the gel. If you do not add EtBr to the gel and running buffer, you will...the DNA in your sample lanes. For more details on doing diagnostic digests and how to interpret them please...typically used to separate 5 - 500 bp fragments. How do you get better separation of bands? If you have similarly...

  28. Common Injection Routes in Mice

    Type
    Blog Post
    ...referred to as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation...

  30. Plasmids 101: Degron Tags

    Type
    Blog Post
    ... protein abundance. Finally, some degron tags can do more than just degrade! Read on to find out more....
Showing: 601 - 630 of 778 results