We narrowed to 728 results for: STO

1. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ...significantly higher than the same prep that has been stored in the freezer. If you plan to freeze a batch of...

2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...patterns in chromatin interactions. The chromatin story told by CAPTURE 2.0 is important, but it’s not the...

3. Tips to Make the Most of a Scientific Conference

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   Blog Post

   ...research, gathered up your data into an exciting story, and are ready to present your findings at a conference...

4. Common Injection Routes in Mice

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   Blog Post

   ...experimental therapies or pain relief, straight to the stomach. This technique involves administering medication...

5. Magnetic Control of Proteins: More than a Dream

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   Blog Post

   ...MagLOV. Andrew was thrilled to see that MagLOV’s “astonishing” magnetoresponse was clearly visible whether ...

6. AAV Vector Quality Control: Going the Extra Mile with NGS

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   Blog Post

   ...addition to genome identification, we developed custom open-access Python scripts that can perform a deeper...

7. Viral Vectors 101: Voltage Indicators

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   Blog Post

   ...a genetic component. The VSD components have historically come from a variety of sources – voltage-gated...

8. Addgene's Cancer Collection Pages Connect the Research Community

   Type

   Blog Post

   ...be to follow up on these promising results and customize the small molecules for a higher affinity fit....

9. Google Forums Round Up: First Impressions of NgAgo

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   Blog Post

   ...in AT-rich regions of the genome or just after a stop codon (as required for C-terminus epitope tagging...

10. Experimenting with New Careers while in Grad School

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    Blog Post

    ...my blog helped immensely when CINSO started brainstorming the resources we wanted to provide online. ...

11. Tips for Using BLAST to Verify Plasmids

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    Blog Post

    ... BLAST options and for our suggested blastx customization. Similar to nucleotide sequences, proteins often...

12. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...containing buffer, polymerase and dNTPs, can be stored frozen: just defrost and add your primers and template...

13. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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    Blog Post

    ...encodes Cas9 and contains a site for inserting a custom gRNA.  While this methodology has proven to be ...

14. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    Blog Post

    ....1709035114 Rubin BE, Diamond S, Cress BF, Crits-Christoph A,  He C , Xu M, Zhou Z, Smock DC, Tang K, Owens...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...in large enough quantities (and the correct stoichiometric ratios) to generate useable data. What’s more...

16. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...known pathogenic alleles, or introduce a premature stop codon to render an RNA nonfunctional. In their quest...

17. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ... 2020. We fit our model for each region using stochastic gradient descent in Pytorch, which gave us the...

18. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...complex biological reagents; many factors, like storage buffers and conditions (3,5,8), injection titers...

19. Course-Based Undergraduate Research Experiences (CUREs)

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    Blog Post

    ...becoming just the fifth repeat Nobel Prize winner in history. Interestingly, he also adds to the disproportionately...

20. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...change your protein of interest.   Fig. 2: Stop re-cutting with PAM and guide RNA disrupting mutations...

21. MXS Chaining

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    Blog Post

    ...Subcellular localization 1 TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag...

22. DNA Purification Without a Kit

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    Blog Post

    ...Before you reach for that silica spin column, stop to consider some ways to purify DNA without a kit....

23. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...you find yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting...

24. Lentiviral Vector Uses and Overview

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    Blog Post

    ...applications of lentiviral vectors! Lentiviral vector history and components In the early 1990s, researchers ...

25. Plasmids 101: Colony PCR

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    Blog Post

    ...cultures of your positive clones. If you want to store your clones longer term, just streak the colonies...

26. CRISPR Antimicrobials

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    Blog Post

    ...targeting a process essential to most bacteria, custom CRISPR antimicrobials can target specific sequences...

27. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    Blog Post

    ...of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine, Department of Microbiology...

28. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ...always get done, but you’ll have time to read a story to your baby or run that extra two miles which is...

29. Lentiviral Vector Guide

    Type

    Guide

    ...VSV-G due to wide infectivity) VSV-G (vesicular stomatitis virus G protein) is the most common envelope ...supernatant containing the viral vectors is removed and stored or centrifuged to concentrate. You can use crude...line protocol . Browse Addgene's collection of in-stock lentiviral preps . CRISPR Genomic Screens Genome-wide...on addgene.org Lentiviral Plasmids Collection In-Stock Lentiviral Preps Addgene's Viral Prep Service Viral... The viral envelope gene; typically vesicular stomatitis virus G glycoprotein (VSV-G), an envelope protein...

30. Transferable Skills Guide: External Collaborations

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    Blog Post

    ...keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I just had...