We narrowed to 849 results for: HA

1. Hot Plasmids - August 2020

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   ...with low affinity variants of TEV. The new system has been demonstrated to work for evolution of other ...

2. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   ... original CAPTURE method is that a new cell line has to be created for each genomic loci of interest. ...

3. An Inside Look into Addgene's Dev Team

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   ...There are many moving parts to our software, which has grown with the company. It would be easy to assign...

4. Designing Your Chalk Talk for the Academic Job Interview

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   ...experiments or a laundry list of molecules to screen has unclear utility. You cannot predict what will happen...

5. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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   ...two flanking repeats. In cells where recombination has not occured, methanol induces the production of the...

6. Tips for a 1st time AAV user (by a Rookie AAV user)

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   ...how to create the iodixanol gradient layers, and has some great pointers that while help you master iodixanol...

7. Simplify Cloning with in vivo Assembly

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   ..., TOP10, and Mach1. No common lab cloning strain has failed so far. What is most important is the transformation...

8. Transferable Skills Guide: Managing a Team

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   ...what to delegate and to whom (make sure that person has time for it!). Then schedule a meeting to discuss...

9. AAVs in Retinal Gene Therapy

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   ...well suited for larger transgenes. Unfortunately it has limited tropism in the retina, primarily infecting...

10. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...be used to determine how long a pool of FP timers has been expressed, either in whole cells or as a pool...

11. Negative Can Be Positive: Open AAV Data with Addgene

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    ...transduced by each serotype. Working at Addgene has shown me this was far from an isolated case. Our ...

12. Bioinformatics at Addgene

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    ... and continuous development of in-house software has been crucial for us to keep pace with our current...

13. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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    ...technology potential enabled by CRISPR-transposons has yet to be fully realized. Many thanks to our guest...

14. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    ...something called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore...

15. Lentiviral Vector Uses and Overview

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    ...expression is sufficient. Lentiviral vector technology has come a long way since the 1990s, both in terms of...

16. CRISPR Antimicrobials

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    ...caveat that phages are very diverse, and each phage has a narrow bacterial host range. To further potential...

17. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...measles, mumps, and rabies. Research on Ebola virus has been limited, in part, due to the necessity for working...

18. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    ...enabled numerous cell biology assays. However, there has been a pervasive view that most FPs in a cell will...

19. Plasmids 101: Control Plasmids

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    ...transfection reagents or the transfection process itself has any cytotoxic effects on the target cells. Another...

20. Negotiating Work and Life: How to Find the Joy

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    ...sometimes put work ahead of life Sometimes, work has to come first. That means you and/or your spouse ...

21. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ...reports which POI band is stronger. The right gel has exactly the same POI bands as the left gel, but because...

22. Twenty Years of Sharing: Expanding Accessibility

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    ...Looking forward Over the past twenty years, Addgene has made huge strides in increasing the accessibility...

23. Twenty Years of Sharing: Addgene's Viral Vector Service

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    ...Addgene have worked hard to ensure that the service has grown steadily, helped by a partnership with the ...

24. CRISPR 101: Validating Your Genome Edit

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    ...effects, you can consider using a version of Cas9 that has been specifically engineered for reduced off target...

25. Filming Science Videos in the Age of Social Distancing

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    ...inch wireless monitor. Wireless video technology has gotten very impressive, but due to costs and availability...

26. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...Bassik lab created a human knockout library that has 10 gRNAs per gene and targets all ~20,500 protein-coding...

27. Replacing Paper: Tips for Choosing an Electronic lab Notebook

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    ...Product Marketing Manager at sciNote. Today, every lab has its own habits and approaches to record keeping. ...

28. Transferable Skills Guide: External Collaborations

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    ...problem or a need for something, usually someone else has realized this too. Begin with informal conversations...

29. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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    ...re-cutting the target sequence once the desired edit has been introduced. We found that blocking mutations...

30. 10 Steps to a Perfect Science Talk

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    ... It makes a great point when I then ask “and who has deposited to the repository?” When most or all of...

31. Thoughts on the Future of Research 2015 Boston Symposium

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    ...Academies define a postdoc as “An individual who has received a doctoral degree (or equivalent) and is...

32. How to Negotiate a Successful Lab Start Up Budget

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    ...means they don’t have the money. If the department has a strict budget, or a lack of resources, then ask...

33. Plasmids 101: E. coli Strains for Protein Expression

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    ... BL21 (DE3) pLysE* Chloramphenicol (pLysE) pLysE has higher T7 lysozyme expression than pLysS; expression...

34. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    ...available at Addgene: psPAX2-D64V. This construct has the D64V mutation, a single amino acid change (from...

35. Antibodies 101: Beyond Surface Labeling

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    ...selecting the primary antibody host species, as it has to be different from the other antibodies in your...

36. Transferable Skills Guide: Conflict Resolution

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    ...organization, because confirming that every person has read and understood every communication that gets...

37. CRISPR-mediated Plant Base Editors

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    ...editors (ABE) What if you have another gene Y, which has a defective allele Ym and you need to convert an ...

38. Fluorescent Proteins 101: When GFP lets you down

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    ...deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum around 488 nm and it emits ...

39. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    ...where the vector that you want to use is very large, has a high GC content, contains a lot of repeats--any...

40. Plasmids 101: Common Lab E. coli Strains

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    ...OpenWetWare's E.coli genotype resource. Additionally, NEB has a great list of genetic markers for your reference...