We narrowed to 920 results for: IRE;

1. Addgene Begins Distribution of Recombinant Antibodies

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   Blog Post

   ...order ready-to-use antibodies for your experiments directly from Addgene. How Addgene is Reimagining Antibody...

2. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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   Blog Post

   ...demonstrate that these results were, in fact, due to impaired endocytosis. In parallel assays designed to test...

3. Hot Plasmids: FRET-Based Biosensors

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   Blog Post

   ...sensor for monitoring calcium versus zinc should require the simple swapping of binding domains, in practice...

4. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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   Blog Post

   ... great if you could make neural silencers that require less power. Those are two of the biggest ones. ...

5. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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   Blog Post

   ...its brightness, and decrease the amount of light required for dimerization. She additionally showed that...

6. Addgene Depositors Get More Citations

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   Blog Post

   ... exploring how innovative tools influence the direction of scientific research. View her research on her...

7. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

   ...limited by costly library prep, as each sample requires an individual library. The plexWell™ workflow ...

8. Creating accessible biology activities in schools with BioBits

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   Blog Post

   ...resources to provide them to their students. My desire to create a set of low-cost, easy-to-use educational...

9. "Build Your Plasmid": The Game - Play to Teach

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   Blog Post

   ...students had fun while applying most of the concepts required by the course and some were so enthusiastic that...

10. Learning Technical Skills at a Lab Central Workshop

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    Blog Post

    ...Clean up  I found this kit easy to use because it required only about 15 minutes of my hands on time and ...

11. Viral Vectors 101: Inverted Terminal Repeats

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    ...ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...

12. What Good Citizenship Can Do for Reproducibility in Science

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    Blog Post

    ...and Joule. In September 2017 she moved away from direct editorial work to focus on a broader role as Vice...

13. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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    Blog Post

    ...capability of sfCherry approximately 20 fold by directed evolution. They then optimized the length of both...

14. Visualizing Genomic Loci with CRISPR-Sirius

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    Blog Post

    ...different chromosomes in living cells, the system requires high-copy chromosome-specific loci in the genome...

15. DAP arrays for multiplex gene editing

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    Blog Post

    ... use, they developed a generalizable method to acquire the most effective DAP array with a given tRNA ...

16. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

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    Blog Post

    ...Addgene. Other plasmids and pAAVs can be ordered directly from Addgene. The Podgorski Lab has provided a...

17. 25 Work From Home Tips for Scientists

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    Blog Post

    ...to your community. For those of you who have the desire and ability to, some communities are looking for...

18. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post

    ...a powerful research tool, but their proper use requires careful amplification and maintenance of good ...

19. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    Blog Post

    ...vehicles, synthetic biology, biosecurity, and the entire 2018 saga of “what’s happening in genetics this...

20. Four Factors that Differentiate the Stem Cell Field

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    Blog Post

    ...When our Director of Biology, Lianna Swanson, suggested that I might be able to attend the 10 Years of...

21. Addgene Welcomes John Doench to the Advisory Board

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    Blog Post

    ...to the Advisory Board. John Doench is Associate Director of the Genetic Perturbation Platform at the Broad...

22. Resume Writing for Non-academic Science Careers

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    Blog Post

    ...Experimental Medicine. She is the Community Relations Director for Curiosity Collider (hyperlink https://curiositycollider.org...

23. A Practical Approach to Choosing the B(right)est Fluorescent Protein

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    ...Plasmids for Subcellular Localization Browse the entire Fluorescent Protein Collection ...

24. Top Requested Lentivirus and AAV of 2016

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    Blog Post

    ...mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs from Bryan Roth’...

25. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...proteins. Affinity purification methods typically require a protein to be tagged, which limits these approaches...

26. 7 Tips to Secure a STEAM Internship This Summer

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    Blog Post

    ...Furthermore, companies across most industries tend to hire their former interns to fill entry-level positions...

27. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    ...plasmids, glycoproteins and other proteins that require post-translational modification may not be easily...

28. Behind the scenes: Addgene’s new search engine and more

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    Blog Post

    ...are many ways a user can input their query — by directly typing terms in a search bar, by ticking some ...

29. 15 Years of Addgene: The Top 15 Plasmids

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    ...lab that contains the gag and pol genes. It also requires pRSV-Rev and the envelope expressing plasmid pMD2...

30. Early Career Researcher Toolbox: Social Media for Scientists

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    ...results we hoped for, but it did open up a new direction for the project. I was invited to participate ...

31. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    ...Although this method shortens construct size, it requires coexpression of Csy4 with Cas9 and the polycistronic...

32. How to Make Friends and Meet People at a Scientific Conference

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    Blog Post

    ...the person you are talking to is really in your direct area.  Review the conference schedule and attendance...

33. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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    Blog Post

    ...is helpful to find peers that share a similar direction or problem. For example, a group of people who...

34. Addgene: The First Twenty Years

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    Blog Post

    ...to be young again! Addgenie Marcy (now our Sr. Director of Production) pie-fully celebrating Addgene's...

35. Antibodies 101: Conjugation

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    ... 2012). And, you can also conjugate antibodies directly to beads for purification purposes.  Coupling...

36. dTAG - You're it!

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    Blog Post

    ...Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids for endogenous gene tagging in...

37. Transferable Skills Guide: Public Speaking

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    ...collaboration and growth opportunities, and gives your entire audience a chance to comfortably engage with you...

38. Transferable Skills Guide: Cross-team Communication

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    ...not so simple Just as our lab used jargon that required context to understand, I came to realize that ...

39. Plasmid Tools for Microbiome Studies

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    Blog Post

    ...transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP). Thus, cells can be both...

40. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    ...endothelial cells from women who are missing an entire X chromosome (TS iPSCs), the lab observed abnormal...