We narrowed to 1,018 results for: REN
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TypeBlog Post... this new technology as helpful as we have! References and Resources Additional resources on the Addgene...Addgene blog: Flies 101: Catching flies in the lab References Abril, U.N.O., et. al. (2022) A plasmid-based...
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Enabling high school research at the Journal of Emerging Investigators
TypeBlog Post...JEI. Chapter 3 - JEI target audience and their current readership. Encouraging students to do science ...biology protocols Check out our molecular biology reference pages ... -
Addgene's eBook Collection
TypeGuide...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help!...you're not sure where to start or need a handy reference, Addgene's eBooks are here to help! 0 of 0 Selected... -
SciComm with the Experts at Science in the News Part 1
TypeBlog Post... the News hosts a podcast, seminars, a blog, conferences, and more for non-scientists. These programs ...Communicating with Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...Top7 receives the Nobel Prize. Created with BioRender.com. Top7 gets its name from “topology of protein...David Baker, Demis Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog Post...research. Listen to learn more about Dr. Kwapata’s current work and thoughts on the need for more scientific...biotech research in Malawi 11:41-14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44... -
PEI Calculator for Planning AAV Packaging Transfections
TypeBlog Post...the calculator in the comments section below. References 1. Choi, Vivian W., et al. "Production of Recombinant...Adeno‐Associated Viral Vectors for In Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed... -
Performing In Vivo CRISPR Screens Using the FITS Approach
TypeBlog Post...activated T cells, precluding analyses of T cell differentiation beginning with naïve T cells. The Framework...improving CD8+ T cell responses to cancer. References and resources Milling, L. E., Markson, S. C.,... -
AAV Packaged on Request is Here!
TypeBlog Post...repository to be packaged in your choice of five different serotypes. We are so excited for all the ways... The AAV Packaged on Request banner. We currently offer serotypes AAV1, AAV2, AVV5, AAV8, or AAV... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog Post... Cervical cancer screening in Ghana Gladys is currently working to enhance cervical cancer screening in...screening for HR HPV alongside the cytological tests currently in use. Gladys will use plasmids containing a ... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...available when they are validated and ready. “Currently, the kit only includes two versions of Cas9, nuclease... system, see Yamamoto and Sakuma’s protocol. Reference: Multiplex genome engineering in human cells using... -
Plasmids 101: How to Name Your Plasmid in 3 Easy Steps
TypeBlog Post...letter abbreviation of the mutated amino acid currently at that position. In contrast, unmutated or Wild... Matthew Ferenc... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...sustainability problem, how can one little change make a difference? Individual changes to our behavior can lead ...energy, so even one less run a week makes a big difference. Clean out freezers so you can make room and ... -
Make a Splash: Notions of Scientific Impact Are Evolving
TypeBlog Post...detailing Nobel Prize-winning research typically weren’t among the most cited. And, as scientific discourse...made of. Increasingly, journal publications alone aren’t enough. Scientists are sharing what they call “... -
A Scientist's Guide to Ebola
TypeBlog Post...virus works its way into immune cells in ways that aren’t fully understood. Much of the action depends on...relevant scientific literature. -In light of the current Ebola crisis, Science and Science Translational... -
Making Career Development an Integral Part of the Science Training System
TypeBlog Post...talent in science related careers. Failure to strengthen the talent pipeline sets the world up for reduced...training programs. I believe in the academic apprenticeship system as a training ground for scientists,... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post... buyer to a seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does...you listed during checkout. This invoice will reference your Addgene order number and PO number that you... -
Inntags: Innovative Protein Epitope Tagging
TypeBlog Post... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small... -
Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton
TypeBlog Post...Research currently under way in the Kotton Lab. One of Darrell's favorite projects - differentiating surfactant... -
What's New in CRISPR - Spring 2019
TypeBlog Post...hisashii, BhCas12b that is functional at 37 °C but preferentially nicks the non-target DNA strand. The lab then...approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these anti-Cas12a proteins... -
Why Do I Need an MTA Anyway?
TypeBlog Post...or choose to auto-approve MTAs. Whatever your preference, we are here to make it fast and easy. Fast....org or +1 617-225-9000, or check out our MTA reference pages. So go ahead and use Addgene plasmids ... -
Bringing Sustainable Practices to the Lab: Innovations
TypeBlog Post... emphasizes Accountability, Consistency, and Transparency. This label scores products by their environmental...pipette tips in a high-throughput and efficient way? Grenova, for example, offers automated, benchtop, high ... -
Reflections and Looking Ahead
TypeBlog Post...find what you need and to bring it in line with current Web Content Accessibility Guidelines (WCAG). These...developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende... -
Phage Directory: From Phage Therapy to a Repository of Phage Information
TypeBlog Post...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... -
Who Gives a Tweet? 9 Facts About Scientists on Twitter
TypeBlog Post...never clicked for me. Recently my interest was renewed in part due to FOMO but mostly because of this.... And don’t forget to follow us @Addgene. References Ke, Q., Ahn, Y., & Sugimoto, C. R. (2017). A ... -
Scientific Sharing in the Time of COVID-19: Databases and Resources
TypeBlog Post...strives to match up scientists across fields with different technical knowledge that could help fight COVID... both clinical care and public health for this current COVID-19 pandemic, and outbreaks that have yet ... -
What's New in CRISPR - March 2020
TypeBlog Post...controls nuclear localization (LINuS). Blue light renders the NLS accessible and the complex enters the nucleus...cycle, asymmetric cell division, and cellular differentiation, but it takes several weeks to delete or deplete... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog Post...the efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce...enhance genome editing capabilities in Drosophila. References: Multiple new site-specific recombinases for ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...Charpentier. I was joined by Addgene Scientist Matt Ferenc (one of our resident CRISPR experts) at the 2014...system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate on what was a “curiosity... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post... just find one that naturally occurs? This is different than the engineered approach used to make eSpCas9...are available from Addgene. Happy CRISPRing! References Harrington, Lucas, et al. "A thermostable Cas9... -
SunTag and Fluorescent Imaging
TypeBlog Post...SunTag? Tanenbaum et al. performed a variety of different experiments in their paper, and so can you! In...we’ll save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post...includes an antibiotic resistance cassette and you aren’t introducing an additional antibiotic resistance...plasmids at Addgene Read our molecular biology reference Watch molecular biology protocol videos ... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog Post...comparison to each other? The gRNAs must target different strands to create a DSB, but this can be accomplished...Shuqi Yan designed experiments to test nickase preferences using HEK293 cells. They started by comparing... -
Build Your CRISPR Vocabulary
TypeBlog Post...nontarget strand. Protein Cas: Different CRISPR systems involve different proteins, but many of them are... -
We're Updating Our Hold for Publication Policy
TypeBlog Post...costly for us to process and store materials that aren’t distributed or for our teams to keep searching,...materials are both excellent ways to provide transparency, support reproducibility, and accelerate science... -
New CRISPR Web Resources and #12DaysofCRISPR Recap
TypeBlog Post...some our most popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR...already has five blue flame plasmids covering three different epigenetic effectors. The diversity of tools is... -
Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development
TypeBlog Post...meiosis in biology. Lots of it is researching the different aspects of the subject you're writing and then...developing new online courses about unique topics that aren’t already offered by eScience Labs. “We work with... -
Bricking Science: Portraying Scientific Reality Through LEGO
TypeBlog Post...standards. Bricking science reminded us of our inner children who have no preconceived obstacles, optimistically...potential, and who sometimes wish they could live differently than we do day to day. We discovered that it ... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...strain or a mutated version of YGOI to test the different phenotypes of the specific mutations. For these..., or knocking down YGOI through RNA-mediated interference (e.g. L4440) and observing the downstream effects... -
10 Ways to Share Your Science!
TypeBlog Post...likely happening in your community, and, if they aren’t, think about launching your own. Close the gap ...anyone looking to make their science matter. If you aren’t ready for a training or one isn’t available yet...