We narrowed to 903 results for: nes

...lot of AAV particles are needed to get a few good ones into a cell to produce expression. The higher the...

...as informed consent (How do we prevent stem cell lines from becoming the new HeLa cells?), genome engineering...

...heard. Here's a template for team meetings. One-on-Ones: Managers should set up regular check-ins with their...

... be done with RNAi in terms of determining what genes are involved in biological process X, you can do...

...those who notice and embrace the “odd and extreme openness” of the lab (i.e projects like the Personal Genome...

... plasmid features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and...

...Lastly, an AAV vector was used to multiplex edit 3-4 genes in a primary culture of mouse neurons and in vivo...

...arrangement of the B-B’ and C-C’ palindromic sequences determines the orientation of the ITR, which can either ...

...function. By using AAVrg in Cre transgenic mouse lines, you can access and manipulate an even further defined...

...ahead for success. Every student should use these guidelines consistently across a number of years as you ...

... alternative initiation, so they mutated the methionines at position 14 and 21 to create GEmCherry2. The...

.... A phage encoding the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate ...

... videos? In this post, we invite you behind the scenes in our content writing process, and to even get...

...Some of these synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA...

...educational content conflicted with plain language guidelines.  Which is why, at the Society for Neuroscience...

...for its immense importance, it may well be what determines whether you get the job after your next interview...

...for bioinformatic data mining. As we continue to harness the natural diversity of CRISPR systems, only time...

....  Before the conference Make sure you have a business card – even students and postdocs. There are many...

...Department at CSU. When not pursuing scientific or business enterprises, he can be found climbing mountains...

...discussed how the regulation of bacterial ORIs determines plasmid copy number within the bacterial cell...

...often have mentoring programs. The Healthcare Businesswomen’s Association hosts an excellent group mentoring...

...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable...

...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular...

...heavy chains. In the lab, this is the region that determines what secondary antibody you should use when performing...

...fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore that are susceptible to redox...

...cloning strategy. Once you've identified potential clones after the screening, sequence the plasmid. Many...

...all-in-one acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition...

... the slide deck (or reminding them to put their phones away), or maybe I was assuming something was intuitive...

... Professor Schuman’s lab showed that FUNCAT’s usefulness can be extended to determine the turnover of ...

...how software can be structured to allow for the messiness of biology. These examples are intentionally trivial...

...generating too many trainees per PI” “Lack of awareness about how the system operates and functions” “...

...limit of roughly 0.1%. Thus, rare, pre-existing clones that are present at less than 0.1% of the total...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...exceptions to our standard workflow.  Table 1: Guidelines for the data fields in the deposit spreadsheet...

... 500 times the number of plaques than the linear ones. The 52-piece circular assembly of the T7 genome...

...S. (2019). DREADDs: The Power of the Lock, the Weakness of the Key. Favoring the Pursuit of Specific Conditions...

...scientists must genotype each animal to identify the ones with the desired genotype. Inducible and conditional...

...maintaining the quality of reagents, especially shared ones.  The importance of QC Plasmids are a fundamental...

... via plasmids or by the creation of stable cell lines and these cells are then grown in biotin-containing...

...t electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful...