...24700457. PubMed Central PMCID: PMC4077948.  Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and...

...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations...

...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage...

... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more...

...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine...

...plasmid. Additionally, the restriction enzyme site(s) allow for the cloning of a fragment of DNA to be ...information and a more extensive strain list. Strain Vendor(s) Genotype BL21 Invitrogen; New England BioLabs E. ...datasheet or the plasmid map to confirm which antibiotic(s) to add to your LB media or LB agar plates. Antibiotic..., or T K G or T M A or C N A, T, C, or G R A or G S C or G V A, C, or G W A or T Y C or T Amino Acids ..., UUC Proline Pro P CCU, CCC, CCA, CCG Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC Threonine Thr T ACU, ACC...SV40 NLS PKKKRKV or PKKKRKVG Protein C EDQVDPRLIDGK S Tag KETAAAKFERQHMDS SB1 PRPSNKRLQQ Webpage and Blog...

...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look...

...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former...

...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent...

... Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. ...

...that the output text files can be hard to read. It´s suggested that you open the above text files, copy...

...applied research since their first use in the early 90’s to genetically modify primary cells. Amongst the different...

...-100 nucleotides long with the desired alteration(s) located in the center of the sequence. Since lambda...

...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ...

...of AAV serotypes, indicating the optimal serotype(s) for transduction of a given organ. Tissue Optimal... OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N. Mol. Ther. 2004 ...

...self-inactivating” (SIN) after integration. Packaging plasmid(s) Envelope plasmid Addgene’s packaging and envelope...one transfer plasmid one or two packaging plasmid(s) one envelope plasmid After media change and a brief... Retroviral integration site selection. Desfarges S, Ciuffi A. Viruses. 2010. Jan;2(1):111-30. PubMed ...

...avenues in healthcare.   References 1. Vinay, Dass S., et al. "Immune evasion in cancer: Mechanistic basis...

...10.1039/c8en01381k Sridharan, R., Zuber, J., Connelly, S. M., Mathew, E., & Dumont, M. E. (2014). Fluorescent...

....17912/DDVH-BG64 Tzur YB, Friedland AE, Nadarajan S, Church GM, Calarco JA, Colaiácovo MP (2013) Heritable...

....3078.  PMID: 22446880; PMCID: PMC3337962. 8. Zhao S, et al. Cell type-specific channelrhodopsin-2 transgenic...