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Find three ways to purify genomic DNA, plasmid DNA, or PCR products without a commercial kit. Also, find a method for regenerating silica columns for reuse.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

Learn how you can use Synthego's ICE tool to analyze your CRISPR genome editing experiments.

Learn about the advantages of secondary nanobodies from the Gorlich lab over traditional antibodies. Applications include one-step fluorescent immunostaining.

Learn about the many resources you can use for career development as a graduate student or post doc.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

Learn about aptamer-based fluorphores and how they can be used to brighten up your experiments.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Learn how to design a CRISPR screening experiment targeting enhancers.

The basics of Fluorescence Resonance Energy Transfer (FRET), including tips and parameters to consider when using FRET for your experiments.

Ten tips to help scientists negotiate their work/life balance from Joanne Kamens, Addgene's Executive Director.

A dive into troubleshooting and optimization steps you can take to improve your western blot or identify and fix issues within the assay.

Learn about what sequencing technologies the quality control team at Addgene uses to maintain a high standard of plasmid and viral vector quality.

Curious about what's new in the Addgene repository this spring? Check out our latest Hot Plasmids post!

Expansion microscopy allows for light microscope imaging of fixed tissues while overcoming the 250 nm resolution limit.

Learn about how to identify and develop creativity in the lab - a skill that's valuable in and out of the sciences!