Hot Plasmids Spring 2024
Type
Blog Post
...many closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes...
We narrowed to 441 results for: %s
-
-
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future... -
Site Directed Mutagenesis by PCR
TypeBlog Post...minor extension can usually ensure that the 3’-base(s) do not form secondary structures. The introduction... -
How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post... tactic, and puts you ahead of most start-up P.I.’s. It doesn’t have to be complicated, or difficult to... -
27 Hot Plasmids from 2016
TypeBlog Post...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...24700457. PubMed Central PMCID: PMC4077948. Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine... -
Luciferase Plasmid Collection
TypeCollection... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. ... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...that the output text files can be hard to read. It´s suggested that you open the above text files, copy... -
Quick Guide to All Things Lentivirus
TypeBlog Post...applied research since their first use in the early 90’s to genetically modify primary cells. Amongst the different... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...-100 nucleotides long with the desired alteration(s) located in the center of the sequence. Since lambda... -
Finding Your Perfect Job After University
TypeBlog Post...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ...