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TypeBlog Post...PMID: 25895059 Barnea, et al. Proc Natl Acad Sci U S A. 2008. PubMed PMID: 18165312 CIDAR MoClo parts ...or clusters. Veggiani, et al. Proc Natl Acad Sci U S A. 2016. PubMed PMID: 26787909 EasyClone 2.0 Yeast...Hanson SM, Rodríguez-Laureano L, Albanese SK, Gradia S, Jeans C, Seeliger MA, Levinson NM, Chodera JD. bioRxiv...
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Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...24700457. PubMed Central PMCID: PMC4077948. Yan, Kelley S., et al. "The intestinal stem cell markers Bmi1 and... -
Harnessing Bacterial Toxins for Allelic Exchange
TypeBlog Post...antibiotics! References Espah Borujeni, Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation ... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...Addgene. Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations... -
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods
TypeBlog Post...: 19363495. 2. Wang JW, Wang A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post... approachable relationship for the mentee, where (s)he feels truly understood and supported, and can more... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...Common gene mutations found in E.coli strains Gene(s) Description Functional Consequence dam DNA adenine... -
Luciferase Plasmid Collection
TypeCollection... in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 64784 pGL3-Basic-IRES Firefly Insertion... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...1:24 - 29:31 - Reproducibility Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former... -
Technologies Enabled by NanoLuc® Luciferase
TypeBlog Post...27786307. PubMed Central PMCID: PMC5476805. 8. Inagaki, S., et al. (2017) Genetically encoded bioluminescent... -
Is this the right place for me? 8 tactics for choosing a lab
TypeBlog Post...do in the future. Questions to ask: Will someone(s) train you technically? Will the PI/supervisor look... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... Kang SH, Bae YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. ... -
CRISPR Guide
TypeGuide...23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou,...Ishiguro, S., Gao, L., Hirano, S., Okazaki, S., Noda, T., Abudayyeh, O. O., Gootenberg, J. S., Mori, H...Chandrasekaran, S. S., Perry, N. T., Schaepe, J., Du, P. P., Lotfy, P., Bassik, M. C., Bintu, L., Bhatt, A. S., & ...PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Scheiman, J., Vora, S., Pruitt, B. W., Tuttle, M., Iyer, E. P. R., Lin, S., Kiani, S., Guzman, C. D., Wiegand..., E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E....Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung... -
Quick Guide to All Things Lentivirus
TypeBlog Post...applied research since their first use in the early 90’s to genetically modify primary cells. Amongst the different... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...-100 nucleotides long with the desired alteration(s) located in the center of the sequence. Since lambda... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...that the output text files can be hard to read. It´s suggested that you open the above text files, copy... -
Finding Your Perfect Job After University
TypeBlog Post...Being a scientist in my late 20’s, new graduates often ask me for advice on careers available to new ... -
Modular Cloning Guide
TypeGuide...Laboratory Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized... Gruetzner R, Ehnert TM, Werner S, Jones JD, Patron NJ, Marillonnet S. A golden gate modular cloning toolbox...vectors specific for tobacco ( N. tabacum ) or potato ( S. tuberosum ). Joint Modular Cloning (JMC) Toolkit ... assembly of single and multi-gene constructs for S. cerevisiae expression. Multiplex Yeast Toolkit (MYT...the MoClo-YTK (Dueber) for genetic engineering of S. cerevisiae , enabling larger multi-gene constructs...transcription units for protein secretion in yeasts S. cerevisiae and P. pastoris (a.k.a. K. phaffii ). ... plasmids for genome integration in fission yeast S. pombe . These can be used with plasmids from the ... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...10.1039/c8en01381k Sridharan, R., Zuber, J., Connelly, S. M., Mathew, E., & Dumont, M. E. (2014). Fluorescent... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...avenues in healthcare. References 1. Vinay, Dass S., et al. "Immune evasion in cancer: Mechanistic basis...