We narrowed to 717 results for: CaS

...fluorescent ligands to HaloTag PCV-Cas9, Cas9-PCV HUH-tag Cas9 plasmids with N- or C-terminal PCV2 HUH-tag...

...will be blocked during protein folding, in which case you should try tagging the opposite end.  If neither...disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... aren’t any good antibodies available. In these cases, having a tagged version of the protein is hugely...

...' decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future of...or are being compelled at the state level, in the case of some public institutions) to cancel plans to ...communication of their plans (many postdocs - and in some cases, faculty - have found out about what their institution...

...entirely, and our software needs to handle these cases. The following code examples show how software can...sample and get it ready for shipping. In the first case, we naively assume that everything will go perfectly...we can interchange them easily as needed. In this case, the physical considerations of the laboratory make...

...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...enough to get a sufficient titer; this is often the case with large genes. This is due to inefficient packaging...inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase ...

...those used for breeding heterozygous mice. In this case, your parental mouse should be hemizygous at the...similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...explained more in Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want ...

...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit.... "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics...blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts...

...other things, science communication comprises broadcasting, science writing, and certain aspects of art...audio or visual person. Why don’t you record a podcast or put some effort into making a video? Use your...Communication Posts Browse our Career Page Listen to Our Podcast Episode on Science Communication Additional Resources...

...they’re knowingly selling a bad product (in which case, you should probably stop using that company). Frequently...frequently asked by customers (and I expect this is the case at most companies). Always go back to the source...that could take weeks to resolve adequately. Occasionally there are some really off-the-wall questions...

...always maintain a backup replicates of each clone in case of contamination. We do not normally use antibiotics...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...potentially large number of single clones obtained (on occasion we have prepared two 48-well plates of clones)...

... matches the copy number of your sample. In that case, an RQ value of one (or, more realistically, between...ddPCR may be a better option for more challenging cases.  Price point If budget is a concern, look for...number and it may not be able to identify trends in cases where the copy number variation is two or less (...

...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing monoclonal ...same hybridoma developed by Evans, et al. In other cases, providers have moved to producing anti-Myc [9E10...

...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

...compared the number of plasmid orders and new COVID-19 cases for each week by location. We observed that as expected...drop dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining...map showing the plasmid orders and new COVID-19 cases each week. Statistical Modeling Note -- If statistical...

...options available to today's science communications: podcasts, blogging, videos, lectures, exhibits, social ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...can increase exponentially with complexity. Podcasts Medium Easy for audience to engage while multitasking...

...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...

...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the primary...indiscriminately, causing competition for binding sites. In cases like these, antibodies can be directly conjugated...simply isn’t available for a specific target. In cases such as this, an epitope such as c-Myc, GFP or V5...

...worry about vector replication at all. In these cases, transient transfection may be sufficient for your...transfection experiments. Reporters Finally, in some cases a researcher may choose not impose selection on ...organism your experiment takes place in. In many cases, you won’t need to worry about the expression of...

...Alongside there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house...knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise unobtainable...patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take the lead and...

...approach known as multimodal therapy. Sadly, in many cases the cancer is diagnosed so late that these treatments...success in treating mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy...and used throughout the world. New mesothelioma cases could be greatly decreased through a ban on asbestos...