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We narrowed to 71 results for: CaS

Showing: 1 - 20 of 71 results

  1. CRISPR Plasmids - Cascade-Cas3

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    ...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with BioRender.com... CRISPR-Cas and Cascade for large genomic deletions. CRISPR...comprised of a combination of Cas5, Cas6, Cas7, Cas8, and Cas11. Cascade is directed to target DNA using... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3 Mammalian...recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling DNA using its helicase properties...this, some CRISPR-Cas3 plasmids take an all-in-one approach and include Cas3 and Cascade on one single plasmid... Resources Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates ...

  2. Plan Your Experiment

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    ...utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be delivered...target cells translate the mRNA encoding Cas to produce the Cas enzyme, and the enzyme then forms a complex...directly combine purified Cas protein and in vitro transcribed gRNA to form a Cas-gRNA complex. You then ... joining (NHEJ) at the cut site Cas9 or Cas9 nickase High-fidelity Cas enzymes increase specificity Dual-nickase...components (Cas enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the...generally only compatible with smaller Cas enzymes, like S. aureus Cas9. For more information, see our blog...deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly into your target cells. In this case, your ...

  3. CRISPR History and Development for Genome Engineering

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    ...prevent the CRISPR-Cas complex from binding to DNA. Others inhibit the recruitment of Cas proteins to the...allowing multiple Cas9s to be used together. Researchers have engineered mutant Cas enzymes with alternative... Fu Y, Sander JD, Reyon D, Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using ...immune system - utilizing various CRISPR-associated (Cas) genes to not only store a record of invading phages...upon re-exposure. More specifically, specialized Cas proteins snip foreign DNA into small fragments approximately...stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process the CRISPR loci... Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material...

  4. CRISPR Plasmids - CRISPR Transposases (CAST)

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    ...two main classes of CASTs are type I and type V-K. Type I systems use a type I Cas effector, composed ...composed of several separate Cas enzymes that form a complex called Cascade ( C RISPR- as sociated c omplex for...target site. Type V-K systems use a type V-K Cas effector, Cas12k, and three transposase components (TnsB,...transposases (Tns) with the precise DNA-targeting of CRISPR-Cas systems, allowing for the integration of large fragments...fragments of DNA at specific locations. The Cas effector lacks nuclease activity, instead recruiting the... CRISPR CASTs CRISPR Plasmids: CRISPR Transposases (CASTs) Mammalian...transposases (CASTs) for insertion of donor DNA. CRISPR...

  5. CRISPR Guide

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    ...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade (see Cas3...Base Editing with CRISPR CasPEDIA: A Functional Classification of Cas Enzymes Cas Enzymes and PAM Sequences...application. Cas9 Orthologs While SpCas9 is the most commonly used Cas enzyme, additional Cas9 orthologs ...editing with Cas3. Browse Plasmids: Cascade-Cas3 Want more information on the wide variety of Cas enzymes?...from. Many variations of Cas9 exist, along with other Cas enzymes like Cas12a, which are available for...paired with the appropriate Cas enzyme. Specific enzymes, such as Cas12a or Cas13 , can improve multiplexing...Other natural Cas orthologs include Hsp1Cas9 (from Helicobacter sp. MIT 11-5569 ) and Nme2Cas9 (from Neisseria...

  6. CRISPR References and Information

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    ... range of Cas enzymes (Cas9 and Cas12) and organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer ...probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology predictor...design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes. Developed by the Manuel...National Laboratory at the National Cancer Institute. Cas-OFFinder (Link opens in a new window) Identifies ...a new window) Labs. Off-Spotter: tool for CRISPR/Cas design (Link opens in a new window) Program for designing... S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your input...vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB...

  7. CRISPR Plasmids - Tagging

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    ...proteins. This CRISPR/Cas plasmid-system consists of two components: A vector containing Cas9 and a validated...marker. The first deposited plasmids in this CRISPR-Cas tagging system were tested by tagging transcription...into human FBL locus Jorgensen Lab SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's...GFP targeting vector. Jorgensen Lab SapTrap CRISPR/Cas Toolkit Kanemaki Lab Auxin-Inducible Degron Tagging...Kanemaki's lab has developed a simple and scalable CRISPR/Cas-based method to tag endogenous proteins by using ...Auxin-inducible degron (AID) tagging with CRISPR/Cas to generate conditional alleles of essential nuclear...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA...

  8. CRISPR Plasmids and Resources

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    ...Engineering Cut Wild-type Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate... generate single-strand DNA breaks. Prime Edit Cas nickase enzymes fused to reverse transcriptase install... DNA without promoting DSB formation. Delete Cascade-Cas3 generates large genomic deletions. Insert CRISPR...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin..., not DNA, across multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  9. CRISPR Plasmids - dCas9-FokI

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    ... CRISPR dCas9-FokI CRISPR: dCas9-FokI...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  10. Rinehart Lab Phosphoprotein Reagents

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    ...for using iSPI, rEcoli XPS, and pSerOTS-C1* (V70) (ca. 2022) from the Rinehart lab: Methods for iSPI, rEcoli...29920078 (Link opens in a new window) Improved Reagents (ca. 2015) described in: Pirman, N. L., Barber, K. W....window) Protocol for using the Improved Reagents (ca. 2015) from the Rinehart lab: Users Guide for Improved...Phosphoprotein Reagents (DOCX, 525.9 KB) Reagents (ca. 2014) described in: Heinemann, I. U., Rovner, A....Link opens in a new window) Protocol for Reagents (ca. 2014) from the Rinehart lab: Users Guide for 2014...Phosphoprotein Reagents (PDF, 691.3 KB) Original Kit (ca. 2011) described in: Park, H., Hohn, M. J., Umehara...

  11. CRISPR Plasmids - Single-Strand Break (Nick)

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    ...Mammalian Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand...of the double-strand breaks created by wild-type Cas enzymes. To use a nickase mutant, you will need two...homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse, sort, or search...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  12. Fluorescent Protein Guide: Biosensors

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    ...Sensor for Ex Vivo and In Vivo Imaging of Ca 2+ Dynamics in High-Ca 2+ Organelles. Cell Chem Biol. 2016 Jun...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin...indicators An Expanded Palette of Genetically Encoded Ca 2+ Indicators. Science. 2011 Sep 8. Robert Campbell...Bcl-2-mediated alterations in endoplasmic reticulum Ca 2+ analyzed with an improved genetically encoded ...Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca 2+ indicators based on computationally redesigned...calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator with enhanced two-photon absorption....green, red, or blue/green) Imaging intraorganellar Ca 2+ at subcellular resolution using CEPIA. Nat Commun...

  13. Plant Plasmids and Resources

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    ...Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base editors, gRNA...MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9-coding gene, zCas9i, and an...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. ... an intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre...CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants - Niko Geldner. CRISPR-Cas9 vector...development. The TapRoot - Podcast (Link opens in a new window) - The Taproot is a podcast to understand how scientific...plasmids expressing different functional variants of Cas9 and empty gRNA backbones. A few examples of useful...

  14. CRISPR Plasmids - Empty gRNA Vectors

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    ...that of the Cas protein present on the plasmid. gRNA plasmids that do not co-express a Cas protein require...more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems... CRISPR requires that you have to express both a Cas protein and a target-specific gRNA in the same cell.... Single plasmids containing both the gRNA and a Cas protein act as an all-in-one vector, but their function... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate... guide RNA expression cassettes and a Cas9 nuclease/nickase expression cassette using the Golden Gate ...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...

  15. CRISPR Plasmids for Genomic Visualization

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    ...CRISPR/Cas plasmids for labeling and visualization of genomic loci.... inactive Cas9 (dCas9) fused to a fluorescent marker like GFP, researchers have turned dCas9 into a customizable...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  16. CRISPR Plasmids - Double-Strand Break (Cut)

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    ...Zebrafish Xenopus Parasite Resources Fully functional Cas enzymes will introduce a double-strand break (DSB...: Overview of NHEJ and HDR following cleavage by Cas9. Browse, sort, or search the tables below for CRISPR...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  17. Zhang Lab CRISPR Page

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    ...Lab General Cloning Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian...Return to top References SpCas9 plasmids Multiplex Genome Engineering using CRISPR/Cas Systems. Cong L, Ran...hosts contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable...efficiency. SpCas9 and SpCas9n with 2a-Puro and 2a-EGFP are also available. 2. SpCas9 (or SpCas9n, D10A nickase...Protocols 2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing ...plasmid expressing SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase...plasmids contain two expression cassettes, a human codon-optimized SpCas9 or SpCas9n, and the single guide RNA...

  18. CRISPR Plasmids - Base Edit

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    ...the fusions, modifying the linker region between Cas variant and deaminase to adjust the editing window...are created by fusing Cas9 nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase...editors are available with multiple Cas9 variants including high fidelity Cas9’s. Further advancements have ...base editors, the evolved TadA domain is fused to a Cas9 protein to create the adenine base editor. Both ... CRISPR-X from the Michael Bassik lab , in which Cas9 is fused to activation-induced cytidine deaminase...CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you ...

  19. Worm Expression Resources

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    ...found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids... via a CRISPR-Cas9 system. Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that...feeding worms bacteria expressing dsRNA or use CRISPR/Cas9 gene editing to easily engineer specific transgenic...worm CRISPR resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous...that incorporate a self-excising drug selection cassette (SEC). More information regarding this kit can...targeting vectors for tag insertion using CRISPR/Cas9 and the selection of C. elegans strains. CRISPR ...that expressed from pHO4d-Cas9 from Michael Nonet. Protocol can be found at the (Link opens in a new window...

  20. Zebrafish Plasmid Collection

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    ...Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...Knock-in tagging - Michel Bagnat lab. Targeting cassettes encoding fluorescent proteins for tagging genes...use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated...
Showing: 1 - 20 of 71 results