We narrowed to 991 results for: Des

...larger Antibody Plasmid Collection , which also includes other types of affinity reagents such as scFvs...Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity Recommended...

...10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed . Description Use of the OPEN method requires OPEN pools (available...using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature...

...identify possible gene function. In this post, I will describe a useful and cost-effective method that our lab...and probe with live cells The following is a description of a technique published by Simpson, Sacher, ...ends) and ligate into a bluntly digested vector designed for protein overexpression in E. coli, such as...the original plate to identify which phage gene encodes that protein! First, block your membranes with ... can lead to high background that obscures the desired signal. We have successfully corrected this by ...

...required, through mitochondrial transfer. In the two decades since this transfer was first proposed, researchers..., 2025). These new sensors use quite different designs, leading to unique advantages. While most red GECIs...two-photon microscopy, and fiber photometry. Plus, both designs offer promising templates for future improvements...al. (2025) under CC BY license. The paper includes other great feats of materials engineering as ... relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside of well-equipped...

...protein analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN ... Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot ...blocking buffers, and incubation times. If the vendor includes publications that cited the antibody, consider...abundance In addition, the thickness of the band provides information about the relative abundance of the...

...protein (Mu- GAM). While many base editors are designed to work in a very narrow window proximal to the... CRISPR Guide Viral Preps CRISPR Protocols gRNA Design Tools CRISPR Blogs CasPEDIA Content last reviewed...

...Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in various serotypes suitable... Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV in various serotypes suitable...

...Voigt Clifford Wang Ron Weiss Synthetic biology describes the rational engineering of biological systems...Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors Zinc Finger Consortium ...

... the work, shared the credit, and we both won accolades that would launch our careers in science. Fast...helped assemble a crack team of Addgenies that includes: Michelle, the Senior Scientist who coordinates... a happier person. What does a good team need? Besides that all-important sense of purpose, there are ...Slack constantly. Whether your teammates are two desks away or on the other side of the world, communication...self-interest ahead of the team’s goals, then that team is destined to do poorly. To some extent, it doesn’t even ...

...critical research infrastructure that has taken decades to establish.These funding decisions will have ...community for years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in ...educational resources: Our eBooks, videos, science guides, and protocols remain freely accessible to all ..., helping teams to be successful in their work despite limited resources. These resources become even ...

...right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself having...want to consult Tables 1 and 2 in our Technical Design of a Western Blot post to make sure you have the...your membrane! Membranes look the same from both sides, so it’s quite easy to think lane 1 is‌ lane 8. ... with smaller tasks, or simply enjoy some well-deserved down time while waiting for your timer to ding...things you’ll want to consider (see our “technical design of a western blot”) post, most imaging issues can....2011.06.001 More resources on the Addgene blog Technical Design of a Western Blot   More resources on addgene.org...

... and the Puromycin resistance gene as separate peptides. PX459 This is the vector backbone. This particular...Protocols Paper for more information). V2.0 This designation separates pSpCas9(BB)-2A-Puro (PX459) V2.0 from... the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann, et al. ...Guide Pages Browse Our CRISPR Collection Find gRNA Design Tools ...

...select the optimal dye or fluorescent ligand when designing an experiment. You may find yourself defaulting...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include..., which is an important consideration for assay design (Breen et al., 2016). When selecting dyes, it's...fluorescent donors and acceptors in their product descriptions, or technical manuals. Specific applications...signal-to-noise ratios. Certain dyes have been specifically designed for use in super-resolution microscopy techniques...

... producing a wall of results that can only be described as bigly. But such a project should not be undertaken...Library Screening Why the kinome? While properly designed perturbations – in the case of screens using CRISPR...References: 1. Doench, John G., et al. "Optimized sgRNA design to maximize activity and minimize off-target effects...with CRISPR/Cas9 Get John Doench's Tips on gRNA Design Check Out News Ways to Use CRISPR for Single Base...

...label of their choice according to the methods described by George et al. (2004). The ACP and SFP Synthases...respectively) may be expressed and purified as described previously in Stachelhaus et al. (1998) References...

...lab earlier, to read that stack of papers on your desk, and to tackle some new experiments before the next...gathering that pile of lab notebooks and binder on your desk. Arrange them in chronological order (or by project...labels. Color code your Post-its and write brief descriptions on each. Place the notes sticking up out of ...colleagues. Start small by identifying the protocols, guides, or references that your labmates ask to borrow...

...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...and industry, often means moving from the lab to a desk, although there’s often technical career tracks ...

... the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some systems including Cas9), Cas...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-... sequences in the CRISPR array that will become guides for future targeted degradation. Before integration...biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources...

...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally... 2022). So the next time you see an antibody description with the Fc domain as only providing structure...

...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...