We narrowed to 475 results for: FLU

1. Hot Plasmids and Viral Preps - May 2021

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   Blog Post

   ...characterized them by ELISA, homogeneous time resolved fluorescence, and suspension bead assay (Figure 2). After...

2. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...protein coding genes in the human genome with fluorescent proteins. For each gene, we needed to design ...

3. Addgene's Cancer Collection Pages Connect the Research Community

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   ...through several different applications such as fluorescent protein fusion studies and the generation of ...

4. PITChing MMEJ as an Alternative Route for Gene Editing

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   ... transgenes. It’s important to note that the fluorescence level observed will be dependent on both the...

5. Anatomy of a Plasmid Page at Addgene

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   ...associated, such as when a plasmid contains certain fluorescent proteins. Finally, for many plasmids you can ...

6. Genome-wide Screening Using CRISPR

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   ... are enriched using either drug selection or fluorescence-based cell sorting and screened for a particular...

7. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...regulation of gene expression including growth or fluorescence. There are a few examples of engineered RNA ...

8. Viral Production

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   Collection

   ...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre....

9. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

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   ...need to use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the...

10. Course-Based Undergraduate Research Experiences (CUREs)

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    ...Histidine tag, malate dehydrogenase, and green fluorescent protein. Biochemistry and Molecular Biology Education...

11. CRISPR 101: Making a Knock-In Cell Line

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    ...through the cell cycle stages. If cells are overly confluent or don’t have sufficient nutrients in their media...

12. Uncovering Ancestral Bias in CRISPR Research

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    ...history of racial bias, which still impacts and influences current research. Understanding the depth and...

13. Tips for arabidopsis transformation

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    ...and C terminally tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (...

14. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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    ... signal amplification in gene expression and fluorescence imaging." Cell 159.3 (2014): 635-646. PubMed...

15. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    ...critical to stem cell survival. Knowing which genes influence the survival of our cell culture is the foundation...

16. Tips for Screening with Yeast Two Hybrid Systems

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    ...protein-DNA and protein-RNA interactions. The 1980s saw a flurry of discovery in the field of eukaryotic transcriptional...

17. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ... is received in the lab, although this number fluctuates throughout the year (see graph below). Addgene...

18. QC Sequencing Technologies at Addgene

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    ...electrophoresis. Capillary electrophoresis reads a fluorescent label on the terminating nucleotide and records...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...you want to fuse your protein to any tags or fluorescent markers? If you’re creating a multigene construct...

20. Plasmids 101: Shuttle Vectors

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    Blog Post

    ...instead to use a vector with a reporter, such as a fluorescent protein, to visualize vector uptake. These reporters...