... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...  Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your...refers to the number of cysteine sites the nanobody has available for conjugation to a specific label (see...

... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...using the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that ...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

... following IV injection. Compared to AAV9, PHP.B has a >40-fold increase in CNS transduction and transduces...adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other Ly6 gene... line of viral vector permissiveness While PHP.B has impressive CNS transduction in some strains of mice...

...fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are also a variety of plant selection...putative T1 transformants: When your T1 is on soil and has recovered from the stress of selection you can clip...cell fate maintenance in the stomatal lineage and has made countless transgenic plants in the process. ...

...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

...months, COVID-19 has had a tremendous impact on our world -- as of June 4, 2020, COVID-19 has caused an estimated...contains requests from 5/22/14 to 5/19/20). Each paper has between 1–32 plasmids and a total of 2–2,590 requests...

...agents. Directly conjugating the primary antibody has the additional benefit of permanent linkage. This...steps following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak... the scope of this post.  We hope that this blog has provided you with a useful overview of the IP method...

...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...

...studying mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult...editing (PE), initially developed in human cells, has been uniquely challenging to apply in dicotyledonous...

...depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb...., or reagents and equipment. Hopefully this post has provided some insight on these differences in viral...

...et al., 2016; Duret et al., 2019). But the field has sometimes seen controversy, and the interactions ...yet to be demonstrated (or invented), its behavior has already been successfully reproduced by several other...tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

...properties of modified histones, a genomic region that has mono-methylated and acetylated histones, but not ...magic and Poisson statistics, each sub-population has, on average, one distinct enhancer disrupted. As ...unhealthy cells. Until recently, the human genome has been full of dark matter: enhancers, lncRNAs, repetitive...

...the State Museum has been the best supporter and most productive partnership CINSO has had. Volunteer! ...provide online. Twitter is an excellent resource that has gained a lot of traction with researchers. It is ...

...cells where binding between the proteins of interest has occurred. Although the original Y2H systems utilized... number of different bait and prey vectors. This has been shown to be as effective as using five independent...transmembrane proteins, a split ubiquitin system has been devised (8). References 1. Casadaban M.J.,...

...but the introduction of type V Cpf1-driven systems has added another option to the CRISPR toolbox. Cpf1’...frequency of desired HDR edits, but the possibility has not yet been experimentally confirmed. To prevent...adaptation of Cas13 to RNA targeting and RNA editing, has further shown the diversity of CRISPR-based systems...

...plasmid we have at Addgene. Each sample at Addgene has four layers of location which can all be extrapolated...hold up to 96 tubes (see image below). Each plate has numbers and letters to give coordinates, from A1 ...replicate sample. The transformation After the DNA has been received and stored, it is put into E. coli ...

...cleavage with some of these peptides, no consensus has been reached on which peptide works best. Likely ... is easiest to start with a plasmid that already has the multicistronic element and reporter cloned in... unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic...

...extraordinary collection of talent, of human knowledge, that has ever been gathered at the White House - with the ... not an excuse. Every psych test I’ve ever taken has declared me an introvert, and I identify as one.  ... on a leaderless team. Sometimes the leader just has to be the person to say “OK, guys, we need to have...anything out the discussion themselves, then one has to question whether that person should be on the ...

... Lab technician / Research assistant Typically has a Master's or Bachelor's degree Generally a short-term...talking to people, though, try to be conscious of who has your best interests at heart. A great piece of advice...vulnerable about my struggles and what I don’t know yet has helped me cultivate stronger relationships with my...academia or industry, so feel free to explore! Addgene has an entire page dedicated to different career options...