Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 856 results for: HA

Showing: 401 - 420 of 856 results

  1. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...research and PhD studies, there lies a hidden gem that has the potential to redefine the course of one's research...inspiration. Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint...

  3. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...preparation in E. coli. Addgene depositor Indu Maiti has created a new and versatile binary Ti vector for ...mosaic virus, the constitutively active M24 promoter has been modified with duplicated enhancer domains and...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...keynote speaker, Dr. Henry Bourne (shown in photo), has been speaking out and writing about issues in our...the same time, he pointed out, academic research has some big problems resulting from past practices and...

  6. Advice for Choosing a Research Project

    Type
    Blog Post
    ...research project can take several years to complete and has multiple benchmarks. Think about what your personal...Have a look at what others have done recently that has been a big hit, and especially look at the new tools...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...overexpression. Tim Formosa, at the University of Utah, has built a complete collection of yeast tagging modules...terminator-(selection marker). Additionally, Dr. Formosa has deposited six plasmids with a multiple cloning site...

  10. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...your research. RNA editing advantages RNA editing has multiple advantages over more traditional DNA editing...will preferentially edit an adenine if the template has a cytosine mismatch at that position. PspCas13b-...edited.   As discussed above, the ability to edit RNA has multiple advantages, including reversibility and ...

  12. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...agents. Directly conjugating the primary antibody has the additional benefit of permanent linkage. This...steps following the incubation step. The wash buffer has an optimal pH and ionic strength that breaks weak... the scope of this post.  We hope that this blog has provided you with a useful overview of the IP method...

  13. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...Synthego’s blog. CRISPR-based genome engineering has revolutionized the gene editing field by making experimental...often under-appreciated or over-looked.  Synthego has developed a new tool called ICE (Inference of CRISPR...increased flexibility and scalability, the ICE software has two analysis formats: sample by sample analysis, ...

  14. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ... a primary antibody. B) Each arm of the antibody has an antigen binding site for a total of 2 binding ...  Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need to express your...refers to the number of cysteine sites the nanobody has available for conjugation to a specific label (see...

  15. MXS Chaining

    Type
    Blog Post
    ... cloning. MXS Chaining is one such technique and has been used to produce complex fluorescent reporter...using the newly generated MCS. MXS-chaining also has the advantage of being directional, meaning that ...culture systems (their original goal). Their work has demonstrated that the MXS-chaining method can be ...

  16. Viral Production at Addgene

    Type
    Blog Post
    ...Addgene has a growing catalogue of viral preps available to request. In this post, we’ll walk you through...below. AAV Production AAV distributed by Addgene has been produced either in-house by Addgene scientists...tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination. In ...

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ... its introduction in 2015 by the Boyden Lab, ExM has been steadily growing in popularity with an abundance...target concentration during expansion. One group has found that using Fab fragment secondaries, rather...experimental approaches. Since it arrived on the scene, ExM has helped researchers gain a deeper understanding of...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... codon table — a chart every molecular biologist has seen before, showing how DNA sequences are translated...lost from the population. A bacterial chromosome has a single origin of replication (ori), where DNA replication... you. Besides the risk to the researcher, Zeocin has been shown to cause mutations even in cells with ...

  19. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...studying mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult...editing (PE), initially developed in human cells, has been uniquely challenging to apply in dicotyledonous...

  20. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...depending on the virus. AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb...., or reagents and equipment. Hopefully this post has provided some insight on these differences in viral...
Showing: 401 - 420 of 856 results