We narrowed to 877 results for: HA

...top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and acrylamide...Choosing your primary antibody Once the membrane has been blocked, it is stained with a primary antibody...the vendor’s website and choose an antibody that has been validated for immunoblotting. Different immunoassays..., cytoplasm and nucleus.  We hope that this blog has provided you with a good overview of the western ...

...last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the genome engineering world by storm owing...that regulate a desired phenotype. In fact, CRISPR has already been used to engineer the genome of many ...to protoplast cells (plant cells whose cell wall has been removed). Another common delivery method is ...developed for plants and prime editing for plants has been developed for rice by Yiping Qi’s lab and for...

...sharing plasmids has become simplified through Addgene. Looking back, Addgene’s journey has been a tough ...

...Hack Day lunches These days everyone is busy and has a To-Do list long enough that there is no hope of...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams. ...

...DRSC/TRiP- Functional Genomics Resources website has in vitro reagents and tools to help you knock down...tools. The Vienna Drosophila Resource Center also has collections of flies for in vivo gene knock down ...finishing his immunology PhD at Harvard University. He has had nearly a decade of experience working with Drosophila...

...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...the bacterial Tn7 transposon system. This system has two parts: a plasmid expressing the Tn7 transposition...

...looked hard at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion...high-throughput design and analysis of CRISPR experiments. It has certainly boosted our productivity in the Biohub....

...methods for CRISPR activation, research in the area has continued to develop. These newer methods have not... of rearranging chromatin structure. This method has shown higher activation levels than SAM in some loci...-HSF activation domains used in SAM. This method has been shown to activate genes at 2- to 3-fold higher...

... of practice to ensure the proper injection site has been located and to avoid potential off-target pokes...researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing...: intermediate Your decision Hopefully this post has provided a good introduction to common injection ...

...Triton X-100, Tween-20, or saponin. Each detergent has different chemical properties that will impact the...always look at your antibody datasheet to see if it has been tested in IF and if a protocol is available....an effective strategy. (This article on fixation has some more examples to help choose which fixative ...

...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting ...Staphylococcus lugdunensis that recognizes a NNGG PAM and has comparable activity to SaCas9. A new yeast inducible...that is smaller and easier to deliver, but so far has few inhibitors identified.     New from the viral...

...available VSDs has been successful even at the single neuron level in vitro. In vivo monitoring has also been...biosensors, and generally as biological tools, our blog has you covered. Happy voltage sensing!   Figure...

...vectors. Addgene depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR ...of the sequence between the microhomologies.MMEJ has certain advantages over HDR. Some species don’t have...the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify due to NHEJ’s dominance ...

...expensive, it may be helpful to find an antibody that has already been validated for flow cytometry through...Characterization through Open Science) initiative has now started producing flow cytometry characterization...overexpression. Additionally, if the cell line used has endogenous levels of expression, this can make the...TIM-1 (Single pass type-1 membrane protein), which has been designated CD365 in the most recent HLDA workshop...as part of the YCharOS open science ecosystem. It has received in-kind support to produce this data from...

...buffer such as TE. You will now have plasmid DNA that has been purified away from the bacterial proteins and...

...bacteria in real time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome... can colonize the mammalian intestinal tract and has been used for 100 years to clinically treat enteric...

...about whether Natronobacterium gregoryi Argonaute has any DNA editing functionality Collecting preclinical...extension also tells readers if a paper they’re reading has been linked in a ReFigure making it easy for others...

...research and PhD studies, there lies a hidden gem that has the potential to redefine the course of one's research...inspiration. Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint...

...cooperative education experience (co-op) is what has brought me, a second year bioengineering student,... that remote work would feel isolating, but that has not been the case for me at Addgene. There is a clear...

...manipulated, its anatomical origin, if cell line has ever been the target of an ‘-omics’ study or is resistant...can be used in some cases, but much of this work has required manually going through the pdfs of the 26,000...