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We narrowed to 430 results for: HAL

Showing: 401 - 420 of 430 results

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Tips for CRISPR Gene Editing in Mice

Type

Blog Post

...stranded break at the target site, allowing the two halves of EGFP to recombine by homology directed repair...
Important Considerations in Optogenetics Behavioral Experiments

Type

Blog Post

...than ICSS. The test chamber is divided into two halves that are distinguished visually through different...
Tips for Using FRET in Your Experiments

Type

Blog Post

...pointing out a group of students in a university hall but not knowing if these students know or interact...
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Type

Blog Post

...distance is only 10 bp, the efficiency already drops by half and after only about 30 bp, it is no longer feasible...
Harnessing Bacterial Toxins for Allelic Exchange

Type

Blog Post

... revolutionized allelic exchange. Even now, only half our lab has switched over to pTOX. However, we feel...
Hot Plasmids: Summer 2024

Type

Blog Post

...myriad cell types and subtypes, and a familiar challenge for anyone who has done histology on brain tissue...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...capacity and speed of cells. (a) With each division half of the dye molecules are lost and the fluorescence...
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

Type

Blog Post

... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...
CRISPR 101: Homology Directed Repair

Type

Blog Post

...CRISPR forum moderated by the Zhang lab CRISPR Challenges: Standardization and HDR CrispyCrunch Optimizing...
Transferable Skills Guide: Conflict Resolution

Type

Blog Post

...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...
Filming Science Videos in the Age of Social Distancing

Type

Blog Post

...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...
New Optogenetic Tools for Cytoskeleton and Membrane Control

Type

Blog Post

... Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., & Hehnly, H....
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Type

Blog Post

...soluble and insoluble proteins, which can be a challenge for classic antibody based affinity purification...
Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Type

Blog Post

...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...
Viral Vectors 101: AAV Variables That Matter

Type

Blog Post

...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https:/...
Plasmids 101: Dimers and Multimers

Type

Blog Post

... PMID: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of...
Plasmids 101: Repressible Promoters

Type

Blog Post

...solve the problem of broad QF expression-induced lethality in flies. Riabinina et al. also developed chimeric...
A Control for All Seasons

Type

Blog Post

...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...
9 tips for a successful postdoctoral experience

Type

Blog Post

...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...

Showing: 401 - 420 of 430 results

Results per page:

Tips for CRISPR Gene Editing in Mice

Important Considerations in Optogenetics Behavioral Experiments

Tips for Using FRET in Your Experiments

3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

Harnessing Bacterial Toxins for Allelic Exchange

Hot Plasmids: Summer 2024

Antibodies 101: Beyond Surface Labeling

SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

CRISPR 101: Homology Directed Repair

Transferable Skills Guide: Conflict Resolution

Quick Guide to Near-Infrared Fluorescent Proteins

Filming Science Videos in the Age of Social Distancing

New Optogenetic Tools for Cytoskeleton and Membrane Control

Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

Viral Vectors 101: AAV Variables That Matter

Plasmids 101: Dimers and Multimers

Plasmids 101: Repressible Promoters

A Control for All Seasons

9 tips for a successful postdoctoral experience

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