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We narrowed to 8 results for: HAL

Showing: 1 - 8 of 8 results
  1. Optogenetics Guide

    Type
    Guide
    ...the cryptophyte Guillardia theta ). Halorhodopsins Halorhodopsins are light-gated, inward-directed chloride...chloride pumps isolated from halobacteria. Wild-type halorhodopsin, known as NpHR (from Natronomonas pharaoni...light-driven inward chloride pump from Haloarcula (Halobacterium) salinarum (strain Shark), used to silence...Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense and its variants are commonly used ...reinhardtii Other Channelrhodopsins Anion Channels Halorhodopsins Archaerhodopsins Leptosphaeria Rhodopsins These...variant; increased photocurrent amplitude 517 Halorhodopsins Name Description Peak excitation λ (nm) Jaws...silence neuronal activity 632 Halo/NpHR Light-driven inward chloride pump from Natronomonas pharaonis ,...
  2. CRISPR Guide

    Type
    Guide
    ...less widely used in bacteria due to technical challenges, bacterial CRISPR libraries have been developed... S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G....., Gootenberg, J. S., Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp...790. PMID: 38216671 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...Communications . 9 (11), e1007749. PMID: 30575746 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...cells. Science . 343 (6166), 84–87. PMID: 24336571 Shalem, O., Sanjana, N. E., & Zhang, F. (2015). High-throughput...Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015). CETCh-seq: CRISPR...
  3. Lentiviral Vector Guide

    Type
    Guide
    ...Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production Your Lentiviral Plasmid...https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh, H. E. G., Bolandian, M., Dorostkar, R., Jafari....org/10.1016/j.omtm.2020.10.012 PMID: 33768124 Marshall, H. M., Ronen, K., Berry, C., Llano, M., Sutherland...https://doi.org/10.1089/hum.2012.229 PMID: 23311447 Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott...
  4. Adenovirus Guide

    Type
    Guide
    ...provide the deleted adenoviral genes. The biggest challenge of the HCAdV vector production is to ensure no... protect nonhuman primates against ebolavirus challenge . Journal of Virology, 85 (9), 4222–4233. https...
  5. Molecular Biology Reference

    Type
    Guide
    ...site for the next nucleotide (denoted as ddNTPs), halting synthesis after their incorporation. Sanger sequencing...converted back to a regular base and thus do not halt the reaction. Illumina NGS, therefore does not require...
  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...short coding sequences of channelrhodopsins, halorhodopsins, and other optogenetic genes makes them suitable...) and a gRNA together into an rAAV capsid is challenging due to its limited packaging capacity, especially...
  7. Chemogenetics Guide

    Type
    Guide
    ...Slaviero, A. N., Silvagnoli, A. D., Crespo, E. L., Schalau, R., Gott, M., Tree, M. O., Dunbar, G. L., Rossignol...
  8. Plan Your Experiment

    Type
    Guide
    ...generally higher than HDR pegRNA optimization can be challenging Prime Edit Repress or Interfere (CRISPRi) Reduce...
Showing: 1 - 8 of 8 results