We narrowed to 433 results for: Hal

1. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   ...pointing out a group of students in a university hall but not knowing if these students know or interact...

2. Hot Plasmids: Summer 2024

   Type

   Blog Post

   ...myriad cell types and subtypes, and a familiar challenge for anyone who has done histology on brain tissue...

3. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   ...capacity and speed of cells. (a) With each division half of the dye molecules are lost and the fluorescence...

4. New Optogenetic Tools for Cytoskeleton and Membrane Control

   Type

   Blog Post

   ... Ononiwu, F., Pal, D., Manikas, J., Taveras, C., Hall, N. A., Silva, J. D., Freshour, J., & Hehnly, H....

5. CRISPR 101: Homology Directed Repair

   Type

   Blog Post

   ...CRISPR forum moderated by the Zhang lab CRISPR Challenges: Standardization and HDR CrispyCrunch Optimizing...

6. Harnessing Bacterial Toxins for Allelic Exchange

   Type

   Blog Post

   ... revolutionized allelic exchange. Even now, only half our lab has switched over to pTOX. However, we feel...

7. Transferable Skills Guide: Conflict Resolution

   Type

   Blog Post

   ...lack thereof): Information sharing gets more challenging the bigger an organization gets, and creating...

8. Quick Guide to Near-Infrared Fluorescent Proteins

   Type

   Blog Post

   ...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...

9. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   ...small operation, so sometimes it can be a big challenge. But ask any filmmaker or videographer and they...

10. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    ...distance is only 10 bp, the efficiency already drops by half and after only about 30 bp, it is no longer feasible...

11. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type

    Blog Post

    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...

12. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    ...soluble and insoluble proteins, which can be a challenge for classic antibody based affinity purification...

13. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    ...fragments of the chromosome, which was technically challenging. Other homology based technologies We've described...

14. A Control for All Seasons

    Type

    Blog Post

    ...possible due to a lack of available materials, lethality of knockdown, or other factors, consider using...

15. Viral Vectors 101: AAV Variables That Matter

    Type

    Blog Post

    ...Adeno-Associated Viral Vector-Mediated Gene Delivery to the Hypothalamus. Human Gene Therapy, 21(6), 673–682. https:/...

16. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    ... PMID: 32338220. Crozat E, Fournes F, Cornet F, Hallet B, Rousseau P. Resolution of Multimeric Forms of...

17. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    ...solve the problem of broad QF expression-induced lethality in flies. Riabinina et al. also developed chimeric...

18. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type

    Blog Post

    ...such as in the model flowering plant Arabidopsis thaliana (Li et al., 2013; Zhang et al., 2018) and the ...

19. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    ...double helix. In the 1960s, Har Gobind Khorana, Marshall W. Nirenberg, and Robert W. Holley cracked the...

20. 9 tips for a successful postdoctoral experience

    Type

    Blog Post

    ...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...