We narrowed to 768 results for: LIS

...plasmid carrying the required gene.  The table below lists some of the most commonly used selection markers...said element. This link provides a more extensive list of yeast auxotrophic markers and includes the associated...

...group of mentees to get more “bang for the buck”. Listen to our podcast interview with Harvard Medical School... the internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings...

...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...

...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

...experiments The first thing you will need is to get the list of effective gRNA sequences targeting your genes...pool. (Adapted from Wong et al., 2016). With a list of gRNA targeting sequences, you can then readily...

... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt...(2014).   We talked with Voigt about this collision that’s taking place between CRISPR technology ...

... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...

...at the Faculty of Sciences of NOVA University in Lisbon, Portugal. She leads the Glycoimmunology group ...approved by the FDA in 1939 for the treatment of botulism and Lambert-Eaton myasthenic syndrome (LEMS) because...

...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). ...

...but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available... you to fill in module fragments from a dropdown list for every module. Both the modules and the components...

...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...