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We narrowed to 771 results for: LIS

Showing: 401 - 420 of 771 results

  1. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...BASU, is derived from a BirA* homolog found in B. subtilis. BASU was  compared head-to-head with the E. coli...IREB2, which post-transcriptionally regulate iron metabolism pathway transcripts. By using RaPID, Ramanathan...

  2. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...tissues. Although antibody production is well established, it’s also a process that’s difficult for individual...their RANbodies, Yamagata et al used previously published nanobody sequences that targeted the following...

  3. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...incorrect; take it instead as valuable feedback.  Listen. Think about the questions you’ve received, read...read the comments, seek feedback from others, and listen quietly as people discuss their experiences. If...

  4. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...affinity reagents.    Types of Affinity Reagents  (Listed in no particular order.) Fab fragments are created...distinction of being the only affinity reagent on this list derived from nature. Single-domain antibodies are...

  5. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...cytometry’s standard approach is the direct method. ELISAs, immunohistochemistry, and immunocytochemistry ...Introduction to Immunofluorescence Antibodies 101: ELISAs Antibodies 101: Introduction to Antibodies Resources...

  6. Addgene: The First Twenty Years

    Type
    Blog Post
    ...was frustrated by the difficulty of accessing published plasmids from other scientists. She, Kenneth Fan...others could request from — Addgene!  Addgene was established as an e-commerce company, which helped it stay...

  7. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...point, decide early on what to do in a namespace collision. It may even be worth it to have extra columns...solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence, insert, ...

  8. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...consider—we have loaded those we are aware of in our checklist for tissue and cell research. Using cells from...of this literature in our health and medicine checklist. A common worry for researchers wanting to consider...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    ... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... transgenics. A few of the most common uses are listed below. Cre-dependent gene expression – placing...

  12. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...for the administrative side of the grant to be established. Take time to read and fully understand the RFA...achieving each aim. A funded proposal typically lists the deliverables, explains 1-2 potential pitfalls...

  14. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...tool that you are looking for and check our curated list of biosensors too, as another laboratory may have...designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock, L. E., & Eren, A. M. (2024). ...

  16. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available... you to fill in module fragments from a dropdown list for every module. Both the modules and the components...

  17. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...surprised by how multi-purpose it can be. Let’s review a list of MoClo applications. Genetic circuits Most things... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing...

  18. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ... an increase in the complexity. In 2018, they published a paper profiling four base overhang ligation ...fragments may be near the upper limit of what can be accomplished with Golden Gate Assembly—even when getting ...

  19. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...up clear tasks for each member of the team to accomplish so that many elements of the project were able...Addgene Blog Read other Synthetic Biology Posts Listen to Our Interview with Previous Collin's Lab Member...

  20. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...annotation for 90 bacterial elements. By Ppgardne at English Wikipedia, CC BY-SA 3.0, https://commons.wikimedia.org...both polyadenylation and termination. Out of those listed, the SV40 late polyA and rbGlob polyA are thought...
Showing: 401 - 420 of 771 results