We narrowed to 526 results for: MEA;

1. Hot Plasmids - October 2020

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   ...proliferation and had a greater number of red cells as measured by flow cytometry. Cells grown in high IL-3 showed...

2. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ...small size of Acr proteins (~50-200 amino acids) means they can be delivered in vivo using AAV vectors....

3. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ..., Rinaldi)! But leaving a PhD program doesn’t mean you’ve failed. “I got a job as a research assistant...

4. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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   ...and ABE7.10. They quantified genomic editing by measuring the reduction in protein expression of these six...

5. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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   ...that give the strongest biotinylation signal, as measured via Western blot, and that result in proper localization...

6. Lab Automation at Addgene

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   ...about more lab updates as we develop them. In the meantime, if you have any great lab automation tips and...

7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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   ...involve identifying a therapeutic gene, finding a means to deliver it and identifying a suitable route of...

8. Progress Towards a PAM-Free CRISPR

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   ...enzyme recognizes a PAM sequence in your site doesn’t mean it’s suitable for your experiment. If you’re looking...

9. Viral Vectors 101: Chemogenetics

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   ...selective effector molecules (PSEMs). Higher potency means lower drug doses, which decreases the risk of off...

10. Design Tips for Prime Editing

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    ...updated our blog post on Prime Editing, and that meant rereading many of the original papers reporting ...

11. Plasmids 101: The Wide World of Natural Plasmids

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    ...dangerous, they also provide researchers with new means to deliver DNA and other molecules to host organisms...

12. Making CRISPR Plasmids Using Fragmid

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    ...cleaves outside of its recognition sequence. This means that each component can be designed with an overhang...

13. Plasmids for Endogenous Gene Tagging in Human Cells

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    ... for delivery to the cells. As a precautionary measure, it’s also good to make a few single base pair ...

14. Antibodies 101: Stripping and Reprobing Western Blots

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    ...remove some of your sample —and harsher stripping means more sample loss. If you are still optimizing your...

15. Engaging with science and society at pgEd

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    ... of genetics and our consistent stream of events mean that there is never a dull day in the office. And...

16. Overcoming the Challenges of Lentiviral Production

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    ..., at Addgene we typically concentrate 100-fold meaning that a 10 cm2 dish preparation of virus will only...

17. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    ... poster should be legible from a distance. This means big font and fewer words. Further, the poster should...

18. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...faster in the following test and skew results measuring locomotion. Mice may need to be given some time...

19. Mouse Modeling, Part 2: Breeding and Crossing Mice

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    ...alleles, such as Cre, function when hemizygous, meaning that only one chromosome needs to contain the transgene...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...independently of dCas9 targeting (Galonska 2018). This means that off-target loci – in neighboring chromatin ...