We narrowed to 532 results for: PAN

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   ... any of the error-prone repair that normally accompanies Cas9 cutting. We still don’t know the mechanism...

2. Addgene Welcomes John Doench to the Advisory Board

   Type

   Blog Post

   ...barely understood.” Doench’s graduate work helped expand our understanding of how miRNAs regulate their ...

3. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...engineering, sequencing, and translation of results to companies.” Take home message Starting a lab can be a challenging...

4. A Novice's Perspective on Neuroscience - SfN2017

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   Blog Post

   ...neuroscience trade. There were, of course, many companies selling antibodies, microscopes, and dissection...

5. A Deep Dive into BioBuilder

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   Blog Post

   ...experiments.  As a project-based curriculum, BioBuilder expands learning outcomes by emphasizing the importance...

6. Antibodies 101: Monoclonal Antibodies

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   Blog Post

   ...antibodies. Finally, the selected hybridoma is expanded as a clonal population (Holzlöhner et al., 2017...

7. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

   Type

   Blog Post

   ..., the Sidhu group at the University of Toronto expanded the specificity landscape of the SH3 proteins ...

8. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   ...grant proposals to be reviewed by interdisciplinary panels.  And highly successful scientists need to communicate...

9. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   ...following articles are just a small cross-section spanning 20 years of research. DNA Cross-Bridging Shapes...

10. Building and Validating Recombinant Antibodies: A Case Study

    Type

    Blog Post

    ...make them publicly available. The lab has also expanded their recombinant affinity reagent tools to include...

11. Hot Plasmids - February 2022

    Type

    Blog Post

    ...Pertubation Platform at the Broad Institute have expanded the genetic screening toolkit by optimizing a ...

12. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type

    Blog Post

    ...and learn from them. I started at my previous company by taking on responsibilities such as mentoring...

13. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type

    Blog Post

    ...read flow plots and have designed your first flow panel, you’ll load your samples into the cytometer and...

14. Viral Vectors 101: The AAV Retrograde Serotype

    Type

    Blog Post

    ..., 2022) and substantia nigra (Tervo, 2016). Discrepancies in transduction efficiency can have a major ...

15. Viral Vectors 101: Viral Applications

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    Blog Post

    ...AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells Additional Resources...

16. How Addgene Creates Content

    Type

    Blog Post

    ...with microscopy and flow cytometry experts for the panel recommendations. A few discussions in the review...

17. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    ...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

18. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    ... have time to really connect with others whose company they enjoy on a professional and personal level...

19. Plasmids 101: Yeast Vectors

    Type

    Blog Post

    ...vector propagation. This post, along with a future companion post on mammalian vectors, will catch you up on...

20. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type

    Blog Post

    ... Provide perspective on core values at another company/department Give more candid feedback and be able...