We narrowed to 653 results for: PPL
-
TypeBlog Post...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...
-
Technique: Probe Phage Genomes for Host Binding Proteins
TypeBlog Post...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...extensively characterized and validated, with the application data from IPI and other labs easily viewed in...2021). Directed Evolution: Methodologies and applications. Chemical Reviews, 121(20), 12384–12444. https... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...regions flanking the target site). The best applications for dsDNA inserts include: large insertions ...Uses in Recombineering Substrate Best Suited Application Recombination Frequency Special Considerations... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...sequencing is laborious, time consuming and cannot be applied to heterogenous populations (Bell et al. 2014)....Genotyping Options Technique Detection Limit Application Advantages Disadvantages GUIDE-Seq 0.1% Cell-based... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct comparative... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...editing typically needs to be optimized for each application, and the efficacy varies widely — it still almost... unedited strand (Chen et al., 2021). The team applied this approach to both the PE2 and PE3 systems, ... -
Technical Design of a Western Blot
TypeBlog Post...published antibodies, check the manuscript and supplementary figures for validation data, or follow the references...it includes validation data relevant to your application — and double-check that lot number while you’... -
New Western Blot Protocol Video!
TypeBlog Post... Western blot and share tips for this tricky application. You can check out all of our protocols ... -
It’s CRISPR Week at Addgene!
TypeBlog Post...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress... -
Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes
TypeBlog Post...Callaway For researchers in countries with applicable export control exceptions for rabies and VSV-... -
The Codon Guide to Synthetic Biology
TypeBlog Post...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points... -
Genome Engineering, CRISPR, and Research in Singapore: Interview with Wei Leong Chew
TypeBlog Post...investigator. Coming up with new project ideas, applying for grants, and mentoring. Chapter 5: Wei Leong... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are very grateful for each and... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...novel, the principles that created it have been applied to numerous other protein design goals, both from... -
Bringing Renewable Energy to Addgene
TypeBlog Post...offers options of between 14% - 100% electricity supplied from renewable sources and we have chosen to participate... -
Addgene Protocols Go Viral: AAV Purification and Titration
TypeBlog Post... published an article describing a sucrose supplementation strategy to improve yield. In addition, as ... -
Deck the Lab 2022!
TypeBlog Post... spent a year hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They ... -
Podcast: A Malawian Professor's Path to Biotech Research
TypeBlog Post...Seeding Lab's Positively Instrumental Awards Ceremony Apply to Recieve Equipment through Seeding Labs Resources... -
Call for Guest Bloggers
TypeBlog Post... Molecular biologist with Biogen interested in applying CRISPR for mouse genome editing. Post: Generating...