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We narrowed to 651 results for: PPL

Showing: 401 - 420 of 651 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...indiscriminate ssDNA cleavage activity which cleaves the supplied fluorescently labelled ssDNA substrates. Upon ...fluorescence can be visualised. Another approach was to apply the same concept to lateral flow strips that contain...

  2. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the production...variety of functions via different fusions. Some applications of PUF protein fusions reported in the literature...

  3. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...sequencing. While this may be unnecessary for many applications, it is the only comprehensive method available...you’ve tried everything listed here. For some applications, this is ok, but it still must be controlled...

  4. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...choosing a lab, but almost all of these guidelines can apply to any workplace or job. It amazes me when the most... Is the work (topic and/or technical aspects) applicable to industry if this path interests you  (e.g....

  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...Franklin L., et al. "Revisiting phage therapy: new applications for old resources." Trends in microbiology 23.4...functional genomic and metagenomic screening." Applied and environmental microbiology 74.5 (2008): 1649...

  7. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...sequencing is laborious, time consuming and cannot be applied to heterogenous populations (Bell et al. 2014)....Genotyping Options Technique Detection Limit Application Advantages Disadvantages GUIDE-Seq 0.1% Cell-based...

  8. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review of neuroscience...PMC3225502. 4. Mattis J, et al. Principles for applying optogenetic tools derived from direct comparative...

  10. Technical Design of a Western Blot

    Type
    Blog Post
    ...published antibodies, check the manuscript and supplementary figures for validation data, or follow the references...it includes validation data relevant to your application — and double-check that lot number while you’...

  12. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  18. Deck the Lab 2022!

    Type
    Blog Post
    ... spent a year hoarding used tip boxes for a lab supply tree and set a challenge to my lab mates. They ...
Showing: 401 - 420 of 651 results