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We narrowed to 741 results for: REV

Showing: 401 - 420 of 741 results

  1. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can.... Branda, CS and Dymecki, SM. Talking about a Revolution: The Impact of Site-Specific Recombinases on ...

  2. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...based methods for viral titration consider using a reverse transcriptase mutant in a separate control reaction...over-inflate results. A packing vector deficient in reverse transcriptase will produce low levels of virus ...

  3. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ... histones, the more compacted it becomes. This prevents the cellular machinery from physically interacting... further reading and viewing The Epigenetics Revolution: How Modern Biology is Rewriting Our Understanding...

  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...photostability — 25-fold over mVenus and 4-fold over the previous best performer mGold — while maintaining high ... Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR...

  6. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...These benefits of Cas3 do come at a cost — the previously mentioned issues with expressing all Cascade ...evolutionary classification of CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... et al., 2021; Kattnig et al., 2016), and some previous attempts at magnetic control of biological systems...exist at all. Andrew and Maria were encouraged by previous reports that described magnetic responses of cryptochrome...

  10. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...before entering the hood is another great way to prevent contamination. This sounds rather trivial, but ...closed when they aren’t in immediate use. This will prevent spills, but, if any spills do occur, they should...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

  12. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture...proteins at endogenous loci, shedding light on previously unknown protein functions. References Schwartz...

  15. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...by encapsulating a single enzyme. Compared with previous research, which required protein compartments ...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ... the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, Cu= Cuneate tract, dCST=...large field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features...

  20. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...submitted by researchers like you. Reports are reviewed by Addgene scientists for clarity and accuracy...recombinant AAV2 particle aggregation and methods to prevent its occurrence during vector purification and formulation...
Showing: 401 - 420 of 741 results