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We narrowed to 782 results for: SCR;

Showing: 401 - 420 of 782 results

  1. Viral Production at Addgene

    Type
    Blog Post
    ...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

  2. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...enrichment (SELEX), some 1010 oligonucleotides could be screened for their affinity to a wide range of non-nucleotide... as the aptamer is bound to a protein fluor. Transcribing and translating a protein fluorophore also takes...

  3. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...the results of studies on reducing antibiotic prescriptions for respiratory tract infections. The video ...provide more than enough time for researchers to describe their science, and for viewers to decide if reading...

  4. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...calendar invite over to the r/Science team with a description of our AMA and Joanne’s preferred time to start...receive via email. The cool features are too many to describe here, but I have over 2,000 notes which Evernote...

  5. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ... lines you are using, it is best to start from scratch. Obtain a vial of the desired cell line from a ...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...positive control, the internal control vector, was described earlier. Once you are sure your conditions are...come from multiple, independent sources. We'll describe these a bit more below, but the papers cited in...

  7. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive) conflict...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

  8. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ... from a gene synthesis company such as IDT or Genscript, and cloning them sequentially into the chosen...combining the AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield...

  9. Five Popular Model Organisms

    Type
    Blog Post
    ...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...unicellular organisms, they can also be used to easily screen multiple therapeutic compounds in one experiment...

  11. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...) to remove mutated exons from the dystrophin transcript. Unfortunately, the oligonucleotides only modestly...in one experiment, they found the exon-skipped transcript constituted 59% of total dystrophin mRNA, similar...

  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...”, “colony”, etc. This will help save time by screening out irrelevant candidates, and, more importantly...importantly, it’ll help you define salary costs. 4. Ascribe a cost structure associated to these tools, skills...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants, identifying... insertion, a combined endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to...

  14. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... appealing to researchers.  TALENs In 2010, transcription activator-like effector nucleases (TALENs) were... which is a fusion between Cas9 and reverse transcriptase. Using an elongated template + guide RNA called...

  15. Important Considerations When Using AAVs

    Type
    Blog Post
    ... but many of these tips apply to all tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location...and can immediately undergo replication and transcription. However, with scAAV the effective genome size...

  17. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    .... Bell, Charles C., et al. "A high-throughput screening strategy for detecting CRISPR-Cas9 induced mutations...Learn to Use Cas9 Activators Perform Genome-Wide Screens with CRISPR Generate Mouse Models with CRISPR/Cas9...

  18. Typing CRISPR Systems

    Type
    Blog Post
    ...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...-Aslan, A., & Marraffini, L. A. (2015). Co-transcriptional DNA and RNA Cleavage during Type III CRISPR-Cas...

  20. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... tested and will help healthcare professional prescribe and identify potential beneficiaries. Collaborative...Collection Pages Plasmid Grant to Aid Cervical Cancer Screening in Ghana Modulate Cancer Signalling Resources...
Showing: 401 - 420 of 782 results