We narrowed to 665 results for: SIM

... by a ribonuclease called Dicer. In a great simplification of the process, Dicer chops off the loop of...t always cleave the mRNA — the bound miRISC can simply block translation machinery. The composition of...

...need to know the exact quantity of your POI, but simply whether its concentration changes in response to...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...

...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

...program to design primers for Gibson Assembly. For a simple example of using Gibson assembly, imagine that ...size, and purify the DNA fragments. Finally, you simply incubate these three fragments together along with...

...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...

...advantages and disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads...predominant state of registers in a sample based on a similarity metric that we defined in our paper. In our opinion...

...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism...

...Using in vivo optogenetic stimulations, they simultaneously recorded the electrical impulses of discrete.... PubMed Central PMCID: PMC6010559. Wiegert, J. Simon, et al. "Silencing neurons: tools, applications,...

... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...

... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...

...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...

...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...

...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here...

...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit...

...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...