We narrowed to 658 results for: SIM
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TypeBlog Post...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...
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How to Negotiate a Successful Lab Start Up Budget
TypeBlog Post...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need. Prioritizing resources in your budget The simple practice of putting together a resource budget ... -
Recombinase-based State Machines Enable Order-dependent Logic in vivo
TypeBlog Post...advantages and disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads...predominant state of registers in a sample based on a similarity metric that we defined in our paper. In our opinion... -
PiggyBac-ing Through the Genome Editing Field
TypeBlog Post...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post...Using in vivo optogenetic stimulations, they simultaneously recorded the electrical impulses of discrete.... PubMed Central PMCID: PMC6010559. Wiegert, J. Simon, et al. "Silencing neurons: tools, applications,... -
Finding Your Science Policy Path
TypeBlog Post... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items: A simple place to look for science policy folks is on Twitter... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ... -
Important Considerations When Using AAVs
TypeBlog Post... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog Post...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...cells Cy5NTAc 32 25-45 GFP (fluorescein is similar) Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post... extraordinary ability category The EB-1A - is similar to national interest waiver criteria however the...beneficial during a career search. Doing these simple things will make it easier for you to find and ... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of... -
Using AAV for Neuronal Tracing
TypeBlog Post...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers from the Janelia... -
New Videos: Addgene Lab Tips
TypeBlog Post..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure... -
Twelve Amazing Guest Blog Contributors!
TypeBlog Post...Innovation. An Integrin Antibody Toolkit from IPI Simon Moe, from Promega, who wrote a guide to selecting...