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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 658 results for: SIM

Showing: 401 - 420 of 658 results

  1. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

  2. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...

  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...

  7. Important Considerations When Using AAVs

    Type
    Blog Post
    ... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism...

  8. Finding Your Science Policy Path

    Type
    Blog Post
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...

  11. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...CD45 expression turns out to be weak. Table 2: A simple flow panel to detect live, transduced, and CD45...reduce it whenever possible. Finally, we designed a simple panel to analyze transduced HEK 293T cells. Here...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...://doi.org/10.1371/journal.pone.0016765. Moore, Simon J., et al. “EcoFlex: A Multifunctional MoClo Kit...

  14. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48 38-70 Intact Cells ...80% and 10%. C Cy3NTA and Cy5NTA bind His tags similarly but with differing R0 values, thereby enabling...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...Cas9 (Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of...

  18. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons where...Allen Mouse Brain Connectivity Atlas website. A similar approach was used by researchers from the Janelia...

  19. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ..., add the various reagents to a single tube and simply flick the tube several times. This will help ensure...
Showing: 401 - 420 of 658 results