Skip to main content

We narrowed to 670 results for: SIM

Showing: 401 - 420 of 670 results
  1. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...
  2. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...
  3. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...
  4. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...
  5. Plasmids 101: Biotinylation

    Type
    Blog Post
    ... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...
  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...
  7. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...
  8. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...leaving behind any accessory DNA sequences [2], [3]. Simply put, the piggyBac™ transposon containing a selection...cytotoxic for proliferating cells and provides a simple tool for conditional cell ablation.,” Proc. Natl...
  9. Important Considerations When Using AAVs

    Type
    Blog Post
    ... and anterograde transport, but they also have similar transport characteristics (velocities of movement.... 3. Aschauer, Dominik F., Sebastian Kreuz, and Simon Rumpel. "Analysis of transduction efficiency, tropism...
  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is...Mismatch Cleavage Assay Not Provided 5% Inexpensive, simple Low-throughput, low sensitivity Sanger Sequencing...
  11. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ... assists PI with admin duties Staff scientist: similar role to a postdoc but more permanent Visiting scholar...because they had close relatives suffering from similar diseases. Others were inspired by the imminent ...
  12. Finding Your Science Policy Path

    Type
    Blog Post
    ... short science policy career and this seemingly simple question is a moving target. There are a multitude...pandemic has foisted upon us. Action Items:  A simple place to look for science policy folks is on Twitter...
  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...discussed below. The software could also be used as a simple tool to rapidly find one or a few gRNAs suggested...screen and the appropriate phenotypic readout, similar mapping could be rapidly performed by targeting...
  14. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...infections have resulted in the onset of cancer. Similar patterns of cancer development are observed in ...cell deaths that help activate tumor responses. Similarly, some radiotherapies have caused increased death...
Showing: 401 - 420 of 670 results