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...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated ...fluorescence microscopy and FACS. In an additional approach, plasmids such as pACBSR-hyg and pFLP-hyg will...community organizations and workforce projects to reach audiences beyond our plasmid depositors and requesters...

... our purposes? In search of simple biological machines R bodies can repeatedly extend and contract...using R bodies: simple, force-generating protein machines that can extend and retract over many microns ...with a fluorescent protein remain functional. Attaching the fluorescent protein fusions allowed us to ...the bacteria apart; however, they still remain attached to the ciliate. It’s thought that they function...

...teams of individuals who enjoy and are experts in each of the individual tasks required to develop the ... project gets left undone or is duplicated. With each component of the project completed by an individual...questions. If you are perceived as too busy to be approachable mistakes will be made that could have been prevented...that other people in the team grow and develop to reach their full potential in the organization. This will...

...activities – did you volunteer to do science outreach and teach children about science? Or perhaps you have...employer. For example, my experience in science outreach and writing was how I got my current job as a ...curiositycollider.org/) , which she co-founded, and the Outreach Manager for Science Borealis (hyperlink http:/...

... amongst the team so getting everyone talking to each other cannot be undervalued.” Connie Cepko points...are probably because a PI does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager...message Starting a lab can be a challenging process. Each PI emphasizes the importance of slowly building ...Advice for Choosing a Research Project 9 Tips to Achieve Success in Academia  ...

...all experimental approaches, knowledge of both the advantages and limitations of each Cas enzyme can help...Using a combined random and directed mutagenesis approach, the Anna Cereseto Lab created pX-evoCas9, which...enzymes may better suit specific experimental approaches. One example is CasX, characterized by the Oakes...

...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

... drawbacks with these approaches that makes it challenging for users to achieve targeted diversification... by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria...is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in...

...communication specialist, Rachel, to write. Identify expertise While Rachel had antibody experience, ...they have expertise. Other times, we may need to reach out to an external expert to help us create accurate...about! You can also suggest blog topics or even reach out about writing a guest post (email [email protected]...

...were tested individually in 4 marmosets each. Brain potency for each individual is reported in Figure 4B ... be best served by a dual vector, split intein approach.   Q3: What’s the best method for titering AAV...backbone and so will very likely cross-react with each other. Multiple labs have tried to transfer PHP....

...for expression of the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs... functional VH and VL sequence was not known for each of the mAbs, given the investment it would take ...community with the plasmid sequences available from each plasmid webpage. Importantly, the methods developed...are also secondary antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1...

... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...assays Multiplex assays involve multiple antibodies, each with a different signaling molecule, that all need... Rachel Leeson...

...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing... pregnancy, the researchers were able to examine each individual blastomere to determine its genotype....’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...2016)). During Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

...reproducibility workshops as Director of Scientific Outreach at Code Ocean and through her previous work at...Generating material to empower others to practice and teach reproducibility An early milestone for the group...instruct the workshop at the conference, but because each contributor made the materials accessible, anyone...were assessed and could improve over time. They achieved this by administering pre- and post-workshop surveys...

... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...scientists actually loving the MTA?! Visibility and outreach Scientists know about Addgene and our visibility...increasing internationally, thanks to evolving outreach tactics, increasing business development efforts...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

...finishing your thesis as it is for helping your team reach an important milestone at work.   While I was in...always found that taking a conscious or active approach can be hugely helpful not only for developing ...can start by paying attention to how your mentors reach solutions and ask them to explain their logic. Or...about technical know-how and more about how you approach new challenges.     Know your strengths! Research...

...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

...that they’ll be compatible with your instruments, each other, and your target. I love FPbase’s Spectra ...Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you to detect enzyme activity...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

...originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of... If you want a plasmid for free, you can always reach out to the PI or corresponding author of the paper...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...