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We narrowed to 949 results for: ache

Showing: 401 - 420 of 949 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ... drawbacks with these approaches that makes it challenging for users to achieve targeted diversification... by the Dueber Lab at Berkeley, provides a new approach for generating novel genetic variants in bacteria...is capable of mutagenesis in E. Coli, but this approach requires special bacterial growth conditions in...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...were tested individually in 4 marmosets each. Brain potency for each individual is reported in Figure 4B ... be best served by a dual vector, split intein approach.   Q3: What’s the best method for titering AAV...backbone and so will very likely cross-react with each other. Multiple labs have tried to transfer PHP....

  3. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...for expression of the mAb heavy and light chain, each from a constitutively active CMV promoter. Constructs... functional VH and VL sequence was not known for each of the mAbs, given the investment it would take ...community with the plasmid sequences available from each plasmid webpage. Importantly, the methods developed...are also secondary antibodies that are specific to each subclass. Approximately 70% of mouse mAbs are IgG1...

  4. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    ... which is then read by a detector panel in the machine. Fluorophores are light sensitive, so antibodies...idea to make sure that laser is available in your machine before you make your purchase. Picking a single...assays Multiplex assays involve multiple antibodies, each with a different signaling molecule, that all need... Rachel Leeson...

  5. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type
    Blog Post
    ...viral vector. One example are bacteriophages that attach to bacterial membranes and inject their genetic...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...infected bacterium can either exist as transient extrachromosomal DNA, like a plasmid, or it can integrate into...bacterium to make a small “bridge” or sex pilus that attaches to the recipient cell drawing it close. Once in...

  6. How Addgene Creates Content

    Type
    Blog Post
    ...communication specialist, Rachel, to write. Identify expertise While Rachel had antibody experience, ...they have expertise. Other times, we may need to reach out to an external expert to help us create accurate...about! You can also suggest blog topics or even reach out about writing a guest post (email [email protected]...

  7. Antibodies 101: Conjugation

    Type
    Blog Post
    ...that they’ll be compatible with your instruments, each other, and your target. I love FPbase’s Spectra ...Alkaline Phosphatase (AP). Various substrates for each of these enzymes allow you to detect enzyme activity...to your antibody. This chemical coupling can be achieved through several different mechanisms. Most take...al., 2015). However, there are certainly other approaches that involve modifying the antibody sequence ...

  8. The time and cost required to make a plasmid

    Type
    Blog Post
    ...originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes...screens and the more traditional “one at a time” approach. According to his math, it takes five hours of... If you want a plasmid for free, you can always reach out to the PI or corresponding author of the paper...or changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well...

  9. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...systematically mutated it. Using a robotic picking approach to select cells with variant characteristics they...” vector cloning system - a Gateway compatible approach to simultaneously clone two genes-of-interest ...2016)). During Agrobacterium-mediated DNA transfer, each GOI is usually expressed from a different plasmid...the photoswitchable proteins offers a powerful approach to control protein-protein interactions in cells...

  10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...reproducibility workshops as Director of Scientific Outreach at Code Ocean and through her previous work at...Generating material to empower others to practice and teach reproducibility An early milestone for the group...instruct the workshop at the conference, but because each contributor made the materials accessible, anyone...were assessed and could improve over time. They achieved this by administering pre- and post-workshop surveys...

  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... a moment and honor all of the success we have achieved and the opportunities we have for the future. ...scientists actually loving the MTA?! Visibility and outreach Scientists know about Addgene and our visibility...increasing internationally, thanks to evolving outreach tactics, increasing business development efforts...successful candidates from internal referrals. Our long reaching reputation helps us hire great people from farther...

  12. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...finishing your thesis as it is for helping your team reach an important milestone at work.   While I was in...always found that taking a conscious or active approach can be hugely helpful not only for developing ...can start by paying attention to how your mentors reach solutions and ask them to explain their logic. Or...about technical know-how and more about how you approach new challenges.     Know your strengths! Research...

  13. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...packaging of cargo sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for ...Enhanced prime editing systems by manipulating MMR machinery In their Cell paper, the Liu Lab found that mismatch... developed a system in which the cellular MMR machinery is disrupted via a dominant negative mutation ...intended edit further weakened recognition by MMR machinery and improved editing efficiency. The lab also ...

  14. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...what Shoukhrat Mitalipov and his colleagues have achieved, and how the field will move forward from this...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing... pregnancy, the researchers were able to examine each individual blastomere to determine its genotype....’s important to remember that the study hasn’t achieved any end goal not available with current techniques...

  15. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...containing combinations of different gRNAs in each plasmid. Each gRNA combination can be tracked and quantitatively...continual advancements in gRNA design necessary to achieve maximal on-target and minimal off-target activities...lentiviral plasmids containing one or more gRNAs. As each ligation reaction uses a pool of gRNAs as starting...

  16. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...example, in 2010, less than 15% of postdocs had achieved tenure-track positions within 5-7 years of earning...BEST) awards, which are designed to support new approaches that address the need to train our young scientists...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...PMCID: PMC4304227. 3. Sauermann, Henry, and Michael Roach. "Science PhD career preferences: levels, changes...

  17. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...(MOI) such that each individual cell receives only one barcode. The abundance of each barcoded clone can...using NGS because the error rate of current NGS approaches allows for a detection limit of roughly 0.1%....populations can be eradicated up-front quickly. This approach is in contrast to the current treatment paradigm...

  18. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

    Type
    Blog Post
    ...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...enough regulatory proteins that don’t interfere with each other. CRISPR is so orthogonal and programmable ...guide RNAs connected because the signal degrades at each layer. If we could fix these things, then I think...make orthogonal variation. The first one worked in each case. Sometimes in CRISPR people talk about off-...

  19. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...target sites for base editing (Kim et al., 2017). For each base editor, they observed editing activity with...sites previously difficult to target. ABE8s can achieve 98-99% target modification in primary T cells, ...Grünewald et al., 2020). Another lab took a similar approach. Dali Li’s lab’s A&C-BEmax consists of a fusion...activity compared to ABEmax. In an alternative approach, engineering and directed evolution of the TadA...10.1038/s41587-022-01533-6 Nishida, K., Arazoe, T., Yachie, N., Banno, S., Kakimoto, M., Tabata, M., Mochizuki...

  20. Hot Plasmids February 2024

    Type
    Blog Post
    ...which uses a much shorter crRNA. Cas12-RNP-PAGE achieved high editing frequency (nearly 100% in some cases...method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with multiplexed...successful in primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants...best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier and delivering a transgene...
Showing: 401 - 420 of 949 results