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We narrowed to 716 results for: cas

Showing: 401 - 420 of 716 results

  1. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    ...analysis performed in experiment 1. Use camelCase: In camelCase, each subsequent word in a file name after... more than one word should be named in the camelCaseFormat since spaces between each word can make it ...

  2. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    ...' decisions to alter postdoc salaries - in some cases reversing these decisions entirely. At Future of...or are being compelled at the state level, in the case of some public institutions) to cancel plans to ...communication of their plans (many postdocs - and in some cases, faculty - have found out about what their institution...

  3. To Each HIS Own

    Type
    Blog Post
    ...will be blocked during protein folding, in which case you should try tagging the opposite end.  If neither...disrupted by denaturants (Hochuli, 1988). In some cases, proteins can be bound under denaturing conditions... aren’t any good antibodies available. In these cases, having a tagged version of the protein is hugely...

  4. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...cytometry, secondary antibodies may be indicated on occasion, usually after other signal amplification techniques...indiscriminately, giving them the same output signal. In these cases, it is best to use the direct method when possible...confidently, collect a broader array of data, and occasionally ask themselves and others that most perplexing...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...entirely, and our software needs to handle these cases. The following code examples show how software can...sample and get it ready for shipping. In the first case, we naively assume that everything will go perfectly...we can interchange them easily as needed. In this case, the physical considerations of the laboratory make...

  6. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...single 72h harvest is best for larger genes such as Cas9. See figure 1 for an example of how changing transfection...enough to get a sufficient titer; this is often the case with large genes. This is due to inefficient packaging...inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase ...

  7. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...those used for breeding heterozygous mice. In this case, your parental mouse should be hemizygous at the...similar epigenetic modifications. Special breeding cases Whether your mouse colony consists of genetically...explained more in Part 1 of this series). In any case, Cre is a dominant phenotype, while you’ll want ...

  8. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit.... "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics...blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts...

  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...other things, science communication comprises broadcasting, science writing, and certain aspects of art...audio or visual person. Why don’t you record a podcast or put some effort into making a video? Use your...Communication Posts Browse our Career Page Listen to Our Podcast Episode on Science Communication Additional Resources...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ...they’re knowingly selling a bad product (in which case, you should probably stop using that company). Frequently...frequently asked by customers (and I expect this is the case at most companies). Always go back to the source...that could take weeks to resolve adequately. Occasionally there are some really off-the-wall questions...

  11. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...always maintain a backup replicates of each clone in case of contamination. We do not normally use antibiotics...well plate for further analysis. To assess CRISPR/Cas9 knockout efficacy, we have used both Western blotting...potentially large number of single clones obtained (on occasion we have prepared two 48-well plates of clones)...

  12. Of Myc and Men

    Type
    Blog Post
    ...endoplasmic reticulum localization, Munro and Pelham casually introduced the Myc-tag (Munro, et al. 1986; Munro...developed almost 40 years ago? Well…kinda. In some cases, antibody providers are distributing monoclonal ...same hybridoma developed by Evans, et al. In other cases, providers have moved to producing anti-Myc [9E10...

  13. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... matches the copy number of your sample. In that case, an RQ value of one (or, more realistically, between...ddPCR may be a better option for more challenging cases.  Price point If budget is a concern, look for...number and it may not be able to identify trends in cases where the copy number variation is two or less (...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...individual expression cassettes, having the genes expressed from the same cassette is sometimes advantageous...and you can then insert the whole multicistronic cassette into a backbone as a single unit. Although it ...

  15. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    ...compared the number of plasmid orders and new COVID-19 cases for each week by location. We observed that as expected...drop dramatically when there are many new COVID-19 cases being reported (right, red), likely due to quarantining...map showing the plasmid orders and new COVID-19 cases each week. Statistical Modeling Note -- If statistical...

  16. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...options available to today's science communications: podcasts, blogging, videos, lectures, exhibits, social ...content.  What are you interested in? Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com...can increase exponentially with complexity. Podcasts Medium Easy for audience to engage while multitasking...

  17. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...worry about vector replication at all. In these cases, transient transfection may be sufficient for your...transfection experiments. Reporters Finally, in some cases a researcher may choose not impose selection on ...organism your experiment takes place in. In many cases, you won’t need to worry about the expression of...

  18. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...approach known as multimodal therapy. Sadly, in many cases the cancer is diagnosed so late that these treatments...success in treating mesothelioma, such as in the case of Paul Zygielbaum. A different approach to immunotherapy...and used throughout the world. New mesothelioma cases could be greatly decreased through a ban on asbestos...

  19. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...Alongside there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house...knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise unobtainable...patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take the lead and...

  20. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the primary...indiscriminately, causing competition for binding sites. In cases like these, antibodies can be directly conjugated...simply isn’t available for a specific target. In cases such as this, an epitope such as c-Myc, GFP or V5...
Showing: 401 - 420 of 716 results