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We narrowed to 704 results for: des.1

Showing: 401 - 420 of 704 results

  1. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...part of) figure 1, uploading it as a preprint, or documenting it in some other way. Besides the data, new...protein of interest will be problem-free. In figure 1 we provide an example of the OSER assay with a number...Amsterdam. The previous two posts in this series described a practical approach to selecting a bright fluorescent...

  2. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...a glossary at the end of this post! Figure 1: Base transitions are edits between the purine bases...uracil DNA glycosylase. Nature Communications, 15(1), 4897. https://doi.org/10.1038/s41467-024-49343-5...uracil-DNA glycosylase. Nature Communications, 15(1), 6397. https://doi.org/10.1038/s41467-024-50012-w...an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage...

  3. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...offers insight into a career as a Professor. Step 1: Your PI forwards you a Request For Applications (...proposal typically lists the deliverables, explains 1-2 potential pitfalls of the intended protocol, and...application. It typically includes overview information, key dates, funding description, research plan, award... setbacks, and potential alternative routes, Provides opportunities to convince others why a project...aspects are understood. While reading the funding description, pay attention to the keywords used and think...grant applications restrict the space available to describe the proposed work. Thus, every single sentence...literature and offer educated insight into what the desirable features of future technologies should offer in...

  4. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal...and accurately in a “single pot.’   Figure 1: Single-Pot Assembly: a) Graphic depiction of chromatin...repository that will help epigenetic researchers custom design their own engineering plasmid tools in about a ...cloning, the Haynes lab’s Protocols.io protocol describes an elegant system that allows for the assembly... cells that make up the human body and G-baToN provides versibility in tracking and modulating the interactions...interests via CRISPR techniques. This tool-kit includes an array of FPs as well as additional useful tags...libraries encode target sites flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM...

  5. To Each HIS Own

    Type
    Blog Post
    ...of interest and the metal ions.      Fig. 1: A sample containing a polyhistidine tagged protein... Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins... I place the tag and how many do I need? When designing a plasmid containing a polyhistidine tag, you’...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation...therapies to clinical trials in 2021. Figure 1: Schematic of the human MECP2 locus and MECP2 protein...Tillotson R, Selfridge J, Koerner MV, Gadalla KKE, Guy J, De Sousa D, Hector RD, Cobb SR, Bird A (2017) Radically...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...If you’re new to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role...MECP2 in Rett Syndrome Rett Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who... his observations in a German medical journal. Decades later and after years of painstaking work trying...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...all the colors of the CRISPRainbow. Figure 1: CRISPRainbow was used to track the localization of...is available as a kit from Addgene.   References 1. Chen, Baohui, et al. “Dynamic Imaging of Genomic ...ortholog requires a different PAM sequence, a gRNA designed for one dCas9 should be specific to that ortholog...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...genome editing. Such a system has been previously described for transcriptional activation/repression and ...

  8. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...state of a system that, despite all of its successes, has many deep-rooted flaws (1). As we discovered through... Follow David on Twitter @driglar.     References 1. Alberts, Bruce, et al. "Rescuing US biomedical research...all bad, however - there are some signs that the tides may be turning. In 2013, the NIH announced its first...in Scientific Training (BEST) awards, which are designed to support new approaches that address the need...

  9. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...halorhodopsins to silence neuronal activity (Figure 1) (Wiegert et al., 2017). How to get an opsin into... rather than aversive stimuli. In a recent paper, de Jong and colleagues used rAAV FLEx-based vectors ...brain regions associated with motivated behaviors (de Jong et al., 2019). FLEx vectors represent, therefore...developmental biology at the École Normale Supérieure de Lyon. Her love for educational science led her to...neuroscience 8.9 (2005): 1263. PubMed PMID: 16116447. de Jong, Johannes W., et al. "A neural circuit mechanism...scientific communication manager at genOway. A few decades ago, the brain remained elusive, not from a lack...Boyden et al., 2005; Deisseroth et al., 2006). Despite its relatively recent development, the history ...

  10. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...the lab running like a well-oiled machine. Lab tip #1: Glove trick Putting on lab gloves can sometimes be...distribution. Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your...materials quickly and easily. Learn more about using barcodes. Check out our “Lab Tip” filter on the blog...

  11. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...bioengineering, but it wasn’t clear whether they could 1) be decorated with proteins and remain functional,...Follow her on Twitter @jessicapolka.     References 1. Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. ...of R bodies R bodies have been studied for many decades, and they were known to be resistant to detergents...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...available in your microscope setup. Figure 1: Excitation (dotted) and emission (solid) spectra ...spanning nearly the entire visible spectrum (Figure 1). The GCaMP series (e.g., jGCaMP8) remains the gold... from University of Calgary. Over the past few decades, genetically encoded calcium indicators (GECIs)...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...The main differences between Cas7-11 and Cas13 are 1). non-specific RNA cleavage and 2). cell toxicity.... targeting single stranded RNA.   Table 1: Fast facts on Cas family proteins and genome editing...in one direction. Cas3 is thus not helpful for designer genome edits, but it is effective at wiping out...David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science, 362...

  14. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...company culture.  It’s also a good idea to prepare 1-2 unique questions for each interviewer by looking...to take notes after each interview.   Tailor your 1-hour presentation to your audience Start by asking...method seems simple, I found it hard to remember to describe all four parts of a STAR answer once the interview... because sometimes companies have relaxed dress codes for interviews. You can also ask HR for clarification...

  15. Chemogenetics Guide

    Type
    Guide
    ...bidirectional control of neuronal activity. Table 1. Descriptions of DREADDs and their activity in neurons DREADD...out our Chemogenetics Plasmid Collection . Figure 1: General overview of a chemogenetics experiment. Early... while PSAMs and LMOs have an effect for only 0.5–1 hours after delivery. Chemogenetic ligand. The chemogenetic...a new window) Guettier JM, Gautam D, Scarselli M, de Azua IR, Li JH, et al. (2009). A chemical-genetic...less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...Wess J (2012). Design and functional characterization of a novel, arrestin-biased designer G protein-coupled...

  16. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...impartial. The best science happens when scientists have 1) the courage to make mistakes and 2) the integrity...policies that “muzzle” government scientists to the de-funding of crucial environmental research programs...of what I saw are that we, as a society, need to: 1) use more citations for out data so our sources are...and minority communities, where these issues hit hardest. According to the Guttmacher Institute, most women...was supremely proud of Nashville that morning. Despite the rain, about 4,000 supporters of science showed... an agenda that halts current data collection, destroys existing data, and guts future funding.I marched...science belongs to the taxpayers who fund it, and we deserve to know the truth about our world. Jonathan Aguilar...

  17. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...for locating and containing escaped flies. Nature 4:1-22. https://doi.org/4.01.2022/hafd... research, particularly genetics research, for decades. Its short life cycle, reasonably close genetic...

  18. Participate in the Pets of Addgene Scavenger Hunt

    Type
    Blog Post
    ...scavenger hunt and the subsequent clue. (Begin with clue 1 below!) You will find five photos this way. At the...and have unleashed them all over our recently redesigned blog. 🐕 Your task is to find them, scavenger...

  19. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...basis of many molecular biology tools! Figure 1: Common functions of natural plasmids. Created with....2024.01.039 Garcillán-Barcia, M. P., Redondo-Salvo, S., & de la Cruz, F. (2023). Plasmid classifications. Plasmid...difference is virulence plasmids! This category includes genes that help bacteria invade hosts, including...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others...found on an E. coli fertility plasmid that also encodes a toxin, CcdB, and the resistance to that toxin...
Showing: 401 - 420 of 704 results