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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11 results for: des.1

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Showing: 1 - 11 of 11 results

  1. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex, comprised of a combination of Cas5...endogenous repair mechanisms. Cas3 belongs to the Class 1 family of CRISPR systems, the most abundant type found...bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared to their...still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created with ...

  2. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense), and four transposase components (TnsA, TnsB...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...

  3. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...Link opens in a new window) References Botman, D., de Groot, D. H., Schmidt, P., Goedhart, J., Teusink,...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Fawzi 127194 RP1B FUS 1-163 QQ4xSS #1 FUS His T7 ALS Nicolas Fawzi 127195 RP1B FUS 1-163 QQ4xSS #2 FUS His... type 1 scFv [L24/1] ITPR1 CMV Spinocerebellar ataxia James Trimmer 206790 IP3 receptor, type 1 scFv [... pLKO.1 mTagBFP2 rat DJ-1 shRNA PARK7 U6 Parkinson's Timothy Ryan 223563 pmCherry-Synaptojanin-1-145 SYNJ1...Cookson 13321 pGEX5X.1 PINK1 WT PINK1 GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST...pcDNA3-FLAG-Synaptojanin 1-145 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22292 pcDNA3-FLAG-Synaptojanin...-FLAG-Synaptojanin 1-170 SYNJ1 Flag CMV Parkinsonism, early-onset Pietro De Camilli 22293 pEGFPC1-FLAG-Synaptojanin...FLAG-Synaptojanin 1-145 SYNJ1 GFP, Flag CMV Parkinsonism, early-onset Pietro De Camilli 22294 pEGFPC1-FLAG-Synaptojanin...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...632-675. https://doi.org/10.1038/s44320-025-00109-1 (Link opens in a new window) PMID: 40355756 (Link ...Seuwen, K., Bettembourg, C., Biton, B., Czech, J., de Foucauld, H., Didier, M., Licher, T., Mikol, V., ...

  6. CRISPR Guide

    Type
    Collection
    ...M. M., Semenova, E., Severinov, K., De Vos, W. M., Dame, R. T., De Vries, R., Brouns, S. J., & Van Der...changing the target sequence present in the gRNA. Figure 1: Overview of the basic CRISPR mechanism. CRISPR was...from Staphylococcus aureus ) has a coding sequence ~1 kb shorter than SpCas9 while retaining the same basic...lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...343–347. https://doi.org/10.1038/s41586-022-04470-1 PMID: 35236982 Bubeck, F., Hoffmann, M. D., Harteveld...

  7. Rett Syndrome

    Type
    Collection
    ...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...in transcriptional repression, activity dependent de-repression, chromatin remodeling, gene activation... the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ... cleaves it to destroy the invader ( Figure 1 ). Figure 1: An overview of the endogenous Type II bacterial...separated by short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR...engineering, with Type V following in 2015. Class 1 (Multi-subunit effector complex) Class 2 (Single multi-domain...enChIP) using CRISPR. Biochem Biophys Res Commun . 439(1):132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson...X, Scott DA, Mikkelson T, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. 2014. Genome-Scale CRISPR-Cas9...CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol . 35(1):31-34. PMID: 27918548...

  9. Zhang Lab CRISPR Page

    Type
    Collection
    ...Heidenreich 1, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol . 2015 Jan;33(1):102-6...SpCas9 alone without sgRNA. Full references are below. 1. SpCas9 (or SpCas9n, D10A nickase) + single guide ...screens. The libraries are available in two formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together..., Scott DA, Mikkelsen TS, Heckl D, Ebert BL, Root DE, Doench JG, Zhang F. Science . 2014 Jan 3;343(6166...):186-91. doi: 10.1038/nature14299. Epub 2015 Apr 1. PubMed . Return to AAV In vivo plasmids AAV Plasmids...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ... cell reprogramming factors and wait for cells to de-differentiate. However it may be difficult to decide... with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human...cell reprogramming. Stem Cell Res Ther. 2017 Jun 5;8(1):132. Zovein Replicating EBNA1 episome Human Non-integrating...vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus Mouse Doxycycline-inducible...transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory Neurons Lentiviral...peripheral sensory neurons. Nat Neurosci. 2015 Jan;18(1):25-35. Baldwin Fibroblasts iTSCs Lentiviral Mouse... from Human ALS Patients. Cell Rep. 2016 Jan 5;14(1):115-28. Zhang Adult Dermal Fibroblasts Neurons Lentiviral...

  11. Luciferase Plasmid Collection

    Type
    Collection
    ... Eric Campeau 21474 pLenti CMV V5-LUC Blast (w567-1) Firefly CMV Lentiviral expression of firefly luciferase...-i and neoLux1.2 : Artificial luciferases desined de novo using deep-learning approaches. Split Luciferase...
Showing: 1 - 11 of 11 results