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We narrowed to 634 results for: des.2

Showing: 401 - 420 of 634 results

  1. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...protein products is desired, as multiple selection markers can ensure that all desired tags have been integrated...integrated. Simply design your amplification primers with the desired targeting homology—in frame, of ...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

  2. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... and The CRISPR Software Matchmaker Day 2: Homology directed repair: CRISPR 101: Homology Directed Repair...problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR Genome Editing and...given our CRISPR Plasmids and Resources a fresh new design, sorting the plasmids we have available by category...capabilities. Epigenetics: CRISPR has made it possible to design programmable epigenome editing tools, and the field...cleavage in bacteria. In mammalian systems, Cas13a degrades only the specified RNA target. Bonus: It displays...

  3. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...might otherwise be hard to identify. Figure 2: Users can cross-interrogate screens from the literature...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...

  4. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to maximize activity and ...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini... massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...Institutet, in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000...). Green Listed is a new software tool used to design gRNAs for custom CRISPR screens targeting a (long...moment, 13 full genome SpCas9 knockout libraries designed by different academic labs, targeting mouse, human...drug targets  Another interesting approach is to design screens against known druggable targets, preferentially...

  6. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ...Agrobacterium infection. At approximately 7 kb, pSiM24 is 2 and 6 kb smaller than commercially available pCAMBIA...Improving Agrobacterium binary vectors Vectors designed for use in Agrobacterium are termed binary vectors...ideal for large gene transfer. The M24 promoter provides constitutive, high-level gene expression and is...

  7. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you choose ... favors the breakage of the hydrogen bonds, destabilizing the molecule and favoring the separation of ...nuclease free water is a great option because it provides a great versatility on how to use your plasmid...

  8. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    ...E. coli: acoustic gene 1 (arg1) and acoustic gene 2 (arg2). The key difference between arg1 and arg2 vesicles...have hollow interiors and a protein shell that excludes water but is gas permeable. These vesicles are...sequentially exposed to acoustic pulses with high enough amplitudes to first collapse arg2 and then arg1 vesicles...

  9. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique Detection...PMID: 23792628. PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet...good starting point for most researchers as it provides a list of putative off-target sites that they ... approach misses larger mismatches (e.g. six nucleotides) that may still lead to off-target double-stranded...contains both the rare off target event and the desired edit is low. Therefore, unbiased sequencing may...

  10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...access this tool, set up an IDT account. Figure 2: Addgene's Analyze Sequence tool.   DNA sequence...primer design Design primers for Goldengate cloning   Design primers for In-Fusion Cloning Design primers...traces, while the paid version provides more tools for plasmid mapping and design (Figure 1). Benchling: While...academic users. Serial Cloner: Free desktop-based software for plasmid design and mapping. ApE (A plasmid Editor...Primer design tools Many of the above-listed plasmid mapping tools have built-in primer design tools, ...stand-alone sites for general primer design, as well as primer design for different cloning applications...characteristics. NEB Data-optimized Assembly Design (DAD): This tool designs overhang sets for high complexity Golden...

  11. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...Enhanced CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout... whose expression varies depending on transgene design and delivery. They generated hPSC lines with AAVS1...

  13. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...project my team & I have poured our hearts into for 2 yrs) has 200K+ figures by 95K+ scientists 👨🏽‍🔬👩‍...Image Search results includes images that can’t be reused without attribution, despite filtering for images...Initially I didn’t have access to professional-grade design software, like Adobe Illustrator, and I didn’t ...Googling and some trial and error, I found some free design software that let me create graphics that better...of Adobe Illustrator. There’s both a web and a desktop version of this software. Vectr has layers, which... this post. It’s open source and available for desktop use for Windows, Mac OS X, and Linux. Inkscape ...The web-based collection of over 20,000 icons is designed by professional medical illustrators with input...

  14. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...ratio between the 2 fluorophores, rather than on the fluorescence intensity, it provides a direct and quantitative...CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy for leak-free transcriptional control...Organoid EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated... heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating...biosensors could have a major economic impact.   Design and mechanism of action of the receptor-based SL...targets all mouse genes with 10 gRNAs per gene. The guides are optimized to maximize on-target cleavage and...

  15. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...much has been done with CRISPR technology in just 2 years and excitement about how much more there is ...fly fishing, and local grizzly bear attacks. He described one such recent attack as "hilarious". Welcome... Mehmet Fatih Bolukbasi from Scot Wolfe's lab described a chimeric Cas9 that is improving the nuclease... and foremost, from the custom software used to design complex genetic circuitry to the downstream output...each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also discussed in the book)...also got to show off our new CRISPR page (which describes how to plan your CRISPR experiment for our new...

  16. RUBY-Red Siliques

    Type
    Blog Post
    ...make it easier to study these mutants.   Fig. 2: (A) Betacyanin is produced from tyrosine by the activity... Antibiotic selection would only then kill the desired plants! PCR or fluorescent markers are currently... have similar drawbacks, often requiring the destruction of plants or tissue for GUS staining or are not...from heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore, are useful...from these plants will be green, containing the desired mutation but lacking both RUBY and the construct...compared to other markers lends itself well to nondestructive selection. If RUBY was integrated into a CRISPR...

  17. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...affinity and specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 ...know that the Fc domain provides structure, determines isotypes, and provides a place for secondary antibodies...yes, it does! While the Fc domain’s functions, besides structure and isotype determination, don’t generally... 2022). So the next time you see an antibody description with the Fc domain as only providing structure...

  18. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ... master's degree prepared them for their career.  2. Are you looking for career advancement and growth...a job search website like Indeed. Do the job descriptions sound interesting? Is the work something you... types of jobs people with master's have Job descriptions often fail to capture all of the responsibilities...responsibilities of a role, so it helps to see how people describe their actual work. To do this, try looking at ...and industry, often means moving from the lab to a desk, although there’s often technical career tracks ...

  19. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s ...the region of the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes...et al., 2018). These sensors also exhibit high magnitudes of fluorescent responses. Studies on the kinetics...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors." Science 360.6396...

  20. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...dissociation by bioluminescence resonance energy transfer 2, or BRET2. With binding of the drug, the alpha and...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate...protein signal transducers. The TRUPATH collection provides scientists with a toolkit to gain an understanding...
Showing: 401 - 420 of 634 results